Cell surface morphology of the morphogenetically active system of the embryo after treatment with Tunicamycin, a glycosylation blocking durg

Nedvídek, J; Antalíková, L; Romanovský, A

doi:10.1007/bf01003181

Cited by 1 publication

(2 citation statements)

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“…Romanovsky and Nosek (1980) treated Xenopus laeuis embryos with tunicamycin during early gastrulation and observed a decrease in intercellular adhesivity accompanied by developmental arrest. Similar tunicamycin-induced decreased adhesivity, accompanied by impairment or inhibition of morphogenetic movements at various stages of amphibian development, has been reported by Sanchez and Barbieri (1!383) and by Nedvidek et al (1985).…”

Section: Discussionsupporting

confidence: 81%

“…Many N-linked glycoproteins are located within cell membranes with the oligosaccharide chain extending from the exoplasmic face (Darnel1 et al, 1990). Tunicamycin is known to induce a number of effects on morphogenesis that can be attributed to alterations in cell surface properties believed to be due to glycoproteins (Olden et al, 1978;Romanovsky and Nosek, 1980;Nedvidek et al, 1985).…”

Section: Introductionmentioning

confidence: 99%

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Effects of tunicamycin on retinoic acid induced respecification of positional values in regenerating limbs of the larval axolotl, Ambystoma mexicanum

Johnson

Scadding

1992

Developmental Dynamics

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Urodele amphibians possess a remarkable ability to regenerate limbs following experimental or accidental amputation. Since only those parts of the limb distal to the plane of amputation usually regenerate, this suggests the existence of level-specific positional values within the cells of the limb. Vitamin A and other retinoids respecify the positional values of regenerating limbs such that structures proximal to the actual plane of amputation are formed in the regenerating limb producing proximodistal duplications. Regenerating limbs of larval axolotls OLmbystoma mexicanum) treated with sufficient retinoic acid to induce proximodistal duplication were also treated via implantation with tunicamycin, a drug which blocks the synthesis of glycoproteins by blocking N-glycosylation of proteins. Tunicamycin was shown to inhibit the proximalizing effects of retinoic acid. This indicates that asparaginelinked glycoproteins may be essential to the process through which retinoic acid induces these effects in the regenerating limb and that glycoproteins may be responsible for specifying positional values in regeneration blastema cells.

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Section: Discussionsupporting

confidence: 81%

Section: Introductionmentioning

confidence: 99%