Cell surface phenotype and cytokine secretion in Caco-2 cell cultures: increased RANTES production and IL-2 transcription upon stimulation with IL-1β

Rodríguez‐Juan, Cristina; Pérez‐Blas, Mercedes; Valeri, Anna P.; Aguilera, Nadine S.; Arnaiz‐Villena, Antonio; Pacheco‐Castro, Alberto; Martín-Villa, José Manuel

doi:10.1054/tice.2001.0212

Cited by 14 publications

(9 citation statements)

References 21 publications

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“…After reduction, a violet formazan dye is formed, and the metabolic competence (assumed to be directly proportional to the cell viability) can be quantified spectrophotometrically by comparing the results to the corresponding positive and negative controls [ 53 ]. Caco-2 cells are frequently used as representative cells of the human intestine due to their capacity to spontaneously differentiate after 3–4 weeks into highly polymerised phenotypes [ 54 ] with functional tight junctions in porous membranes [ 55 ].…”

Section: Resultsmentioning

confidence: 99%

Electrostatic Self-Assembled Chitosan-Pectin Nano- and Microparticles for Insulin Delivery

et al. 2017

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A polyelectrolyte complex system of chitosan-pectin nano- and microparticles was developed to encapsulate the hormone insulin. The aim of this work was to obtain small particles for oral insulin delivery without chemical crosslinkers based on natural and biodegradable polysaccharides. The nano- and microparticles were developed using chitosans (with different degrees of acetylation: 15.0% and 28.8%) and pectin solutions at various charge ratios (n+/n− given by the chitosan/pectin mass ratio) and total charge. Nano- and microparticles were characterized regarding particle size, zeta potential, production yield, encapsulation efficiency, stability in different media, transmission electron microscopy and cytotoxicity assays using Caco-2 cells. The insulin release was evaluated in vitro in simulated gastric and intestinal media. Small-sized particles (~240–~1900 nm) with a maximum production yield of ~34.0% were obtained. The highest encapsulation efficiency (~62.0%) of the system was observed at a charge ratio (n+/n−) 5.00. The system was stable in various media, particularly in simulated gastric fluid (pH 1.2). Transmission electron microscopy (TEM) analysis showed spherical shape particles when insulin was added to the system. In simulated intestinal fluid (pH 6.8), controlled insulin release occurred over 2 h. In vitro tests indicated that the proposed system presents potential as a drug delivery for oral administration of bioactive peptides.

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Section: Resultsmentioning

confidence: 99%

Electrostatic Self-Assembled Chitosan-Pectin Nano- and Microparticles for Insulin Delivery

et al. 2017

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“…CCR1 and its ligands play a critical role in the recruitment of inflammatory cells to neurological lesions (33, 34). Hence, infusions of several cell lines with IL-1 α or IL-1 β , including Caco-2, hepatoma, smooth muscle, or astrocytes cell lines (35–38), display enhanced synthesis of CCL3 and/or CCL7, which may interact with its CCR1 receptor. Thus, induction of CCR1 and its ligands in P-NALD-fibroblasts may reflect a common inflammatory response as reported in many neurodegenerative diseases (34, 39).…”

Section: Resultsmentioning

confidence: 99%

The Inflammatory Response in Acyl-CoA Oxidase 1 Deficiency (Pseudoneonatal Adrenoleukodystrophy)

et al. 2012

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Among several peroxisomal neurodegenerative disorders, the pseudoneonatal adrenoleukodystrophy (P-NALD) is characterized by the acyl-coenzyme A oxidase 1 (ACOX1) deficiency, which leads to the accumulation of very-long-chain fatty acids (VLCFA) and inflammatory demyelination. However, the components of this inflammatory process in P-NALD remain elusive. In this study, we used transcriptomic profiling and PCR array analyses to explore inflammatory gene expression in patient fibroblasts. Our results show the activation of IL-1 inflammatory pathway accompanied by the increased secretion of two IL-1 target genes, IL-6 and IL-8 cytokines. Human fibroblasts exposed to very-long-chain fatty acids exhibited increased mRNA expression of IL-1α and IL-1β cytokines. Furthermore, expression of IL-6 and IL-8 cytokines in patient fibroblasts was down-regulated by MAPK, p38MAPK, and Jun N-terminal kinase inhibitors. Thus, the absence of acyl-coenzyme A oxidase 1 activity in P-NALD fibroblasts triggers an inflammatory process, in which the IL-1 pathway seems to be central. The use of specific kinase inhibitors may permit the modulation of the enhanced inflammatory status.

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“…So, mucosal tissues are heavily populated with cells of the immune system and intestinal lining contains more lymphoid cells and produces more antibodies than any other organ in the body [88]. Intestinal epithelial cells also express various cytokines, cytokine receptors and molecules involved in T-cell binding and potentially costimulation [89–91]. Generally mucosal vaccination is used to prevent initial stages of disease as well as to block its development [92].…”

Section: Current Delivery Methods For Biopharmaceutical Proteins Amentioning

confidence: 99%

Oral delivery of human biopharmaceuticals, autoantigens and vaccine antigens bioencapsulated in plant cells

Kwon

Verma

Singh

et al. 2013

Advanced Drug Delivery Reviews

130

146

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Among 12 billion injections administered annually, unsafe delivery leads to >20 million infections and >100 million reactions. In an emerging new concept, freeze-dried plant cells (lettuce) expressing vaccine antigens/biopharmaceuticals are protected in the stomach from acids/enzymes but are released to the immune or blood circulatory system when plant cell walls are digested by microbes that colonize the gut. Vaccine antigens bioencapsulated in plant cells upon oral delivery after priming, conferred both mucosal and systemic immunity and protection against bacterial, viral or protozoan pathogens or toxin challenge. Oral delivery of autoantigens was effective against complications of type 1diabetes and hemophilia, by developing tolerance. Oral delivery of proinsulin or exendin-4 expressed in plant cells regulated blood glucose levels similar to injections. Therefore, this new platform offers a low cost alternative to deliver different therapeutic proteins to combat infectious or inherited diseases by eliminating inactivated pathogens, expensive purification, cold storage/transportation and sterile injections.

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Cell surface phenotype and cytokine secretion in Caco-2 cell cultures: increased RANTES production and IL-2 transcription upon stimulation with IL-1β

Cited by 14 publications

References 21 publications

Electrostatic Self-Assembled Chitosan-Pectin Nano- and Microparticles for Insulin Delivery

Electrostatic Self-Assembled Chitosan-Pectin Nano- and Microparticles for Insulin Delivery

The Inflammatory Response in Acyl-CoA Oxidase 1 Deficiency (Pseudoneonatal Adrenoleukodystrophy)

Oral delivery of human biopharmaceuticals, autoantigens and vaccine antigens bioencapsulated in plant cells

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