Cell Technologies and Tissue Engineering in the Treatmentof Articular Chondral Defects

Sovetnikov, N N; Kalsin, Vladimir A.; Konoplyannikov, Mikhail; Mukhanov, V V

doi:10.17816/clinpract4152-66

Cited by 8 publications

(2 citation statements)

References 22 publications

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“…There is growing recognition of the fact that osteochondral defects of major joints require surgery [14]. In most cases such defects are detected by MRI that enables to choose an adequate replacement technique.…”

Section: Discussionmentioning

confidence: 99%

Replacement of Osteochondral Defects of Major Joints in Experiment

Airapetov¹,

Воротников²,

Venediktov³

et al. 2019

Sovrem Tehnol Med

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The aim of the study was to develop a novel treatment method of knee osteochondral defects consisting in injecting platelet-rich plasma and crushed hyaline cartilage under a collagen membrane, and assess the technique in experiment. Materials and Methods. A prospective study was carried out on small cattle animals, 30 in number, aged 1.5-3 years weighing 20-30 kg. All subjects got a full-thickness defect to the subchondral bone, 4.5 mm in diameter. As a control, one of the joint defects was not replaced. Due to a replacement method, all animals were divided into three groups. One group animals underwent the replacement according to the developed technique: there were used an extracellular collagen matrix and the body resources (platelet-rich plasma and crushed autologous cartilage). Results. The results were assessed 1 month and 3 months after surgery analyzing the type and degree of defect filling. Best results were found in the group, where a defect was covered by an extracellular collagen matrix with platelet-rich plasma and crushed autologous cartilage. The results of the no replacement group were comparable with the findings of other researchers, according to which osteochondral defects almost have no self-regeneration. Conclusion. The suggested replacement technique for osteochondral defect using extracellular collagen matrix, autologous cartilage, and platelet-rich plasma is less aggressive compared to autochondroplasty, and the obtained results are more stable compared to microfracture or tunneling.

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Section: Discussionmentioning

confidence: 99%

Replacement of Osteochondral Defects of Major Joints in Experiment

Airapetov¹,

Воротников²,

Venediktov³

et al. 2019

Sovrem Tehnol Med

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“…Несмотря на относительную простоту биохи-мической и клеточной структуры, способности суставного хряща к регенерации весьма огра-ничены [7], и поиск методов восстановления суставных поверхностей является актуальной и нерешённой задачей [21]. К настоящему време-ни разработан и внедрен в клиническую прак-тику широкий спектр хирургических методик по лечению повреждений суставного хряща: артроскопическая туннелизация, микрофрак-турирование, мозаичная аутохондропластика, пересадка собственной культуры хондроцитов [2,25].…”

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Possibilities of Current Cellular Technologies for Articular Cartilage Repair (Analytical Review)

Bozhokin

Божкова

Нетылько

2016

Traumatology and Orthopedics of Russia

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Despite a wide variety of surgical procedures utilized in clinical practice for treatment of articular cartilage lesions, the search for other options of articular reconstruction remains a relevant and open issue at the current stage of medicine and biotechnologies development. The recent years demonstrated a strong belief in cellular methods of hyaline cartilage repair such as implantation of autologous chondrocytes (ACI) or cultures of mesenchymal stem cells (MSC) including techniques for genetic modification of cells.The purpose of presented review is to summarize the published scientific data on up to date results of perspective cellular technologies for articular cartilage repair that are being developed. Autologous chondrocyte transplantation originally performed by Swedish researchers in 1987 is considered the first clinically applied technique for restoration of hyaline cartilage using cellular technologies. However, the transplanted cell culture featured low proliferative capacity and inability to form a regenerate resistant to high physical activity. Another generation of methods originated at the turn of the century utilized mesenchymal stem cells instead of autologous chondrocytes. Preparation of MSCs is a less invasive procedure compared to chondrocytes harvesting and the culture is featured by a higher proliferative ability. Researchers use various biodegradable carriers (matrices) to secure cell fixation. Despite good clinical mid-term outcomes the transplanted tissue-engineering structures deteriorate with time due to cellular de-differentiation. Next generation of techniques being currently under pre-clinical studies is featured by the preliminary chondrogenic modification of transplanted cell culture. Usage of various growth factors, modified cell product and gene-activated matrices allow to gain a stable regulatory and key proteins synthesis and achieve a focused influence on regenerate's chondrogenic proliferation and in result to form a good hyaline cartilage resistant to high physical load in long term period.Thus, development of methods for articular cartilage repair has long ago went beyond the interests of clinical physicians, and only the close interdisciplinary cooperation of clinicians and specialists for cytology, molecular genetics and, probably, virology would enable replacement of a defect with a rigorous hyaline cartilage.

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Tissue Engineering and Regenerative Medicine Technologies in the Treatment of Articular Cartilage Defects

Басок

Севастьянов

2017

RJTAO

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1 ФГБУ «Федеральный научный центр трансплантологии и искусственных органов имени академика В.И. Шумакова» Минздрава России, Москва, Российская Федерация 2 АНО «Институт медико-биологических исследований и технологий», Москва, Российская Федерация Важнейшими проблемами здравоохранения в индустриальном обществе являются повреждение и деге-нерация суставного хряща, что связано с ограниченными возможностями ткани к регенерации. В обзоре подробно описаны существующие и разрабатываемые технологии восстановления и замещения повреж-денных хрящевых тканей суставов. Дан анализ полученных результатов по двум основным направлени-ям: стимулирование регенерации поврежденной хрящевой ткани и выращивание элементов хрящевой ткани в биореакторах.Ключевые слова: суставной хрящ, клеточно-и тканеинженерные конструкции, регенеративная медицина, биореакторы. TISSUE ENGINEERING AND REGENERATIVE MEDICINE TECHNOLOGIES IN THE TREATMENT OF ARTICULAR CARTILAGE DEFECTS

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Cell Technologies and Tissue Engineering in the Treatmentof Articular Chondral Defects

Cited by 8 publications

References 22 publications

Replacement of Osteochondral Defects of Major Joints in Experiment

Replacement of Osteochondral Defects of Major Joints in Experiment

Possibilities of Current Cellular Technologies for Articular Cartilage Repair (Analytical Review)

Tissue Engineering and Regenerative Medicine Technologies in the Treatment of Articular Cartilage Defects

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