Cell therapy for fibrotic interstitial pulmonary disease: Experimental study

Abstract

Parte superior do formulário Digite um texto ou endereço de um site ou traduza um documento. The aim of this study is to evaluate the histological changes in lung parenchyma of pigs affected by interstitial lung disease induced after the infusion of bone marrow mononuclear cells (BMMCs). Ten female swines were submitted to pulmonary fibrosis induced by a single dose of intratracheal bleomicine sulfate. Animals were arranged into two groups: Group 1: induced-disease control and Group 2: cell therapy using BMMCs. Both groups were clinically evaluated for 180 days. High-resolution computed tomography (HRCT) was performed at 90 and 180 days. BMMC sampling was performed in cell therapy group at 90 days. Euthanasia was performed, and samples were collected for histology and immunohistochemistry. The 90-days HRCT demonstrated typical interstitial lesions in pulmonary parenchyma similarly to human disease. The 180-days HRCT in Group 1 demonstrated advanced stages of the disease when compared with Group 2. Immunohistochemistry analysis suggests the presence of pre-existent vessels and neoformed vessels as well as predominant young cells in the injured parenchyma of Group 2. Immunohistochemistry analysis suggests that cell therapy would promote a reconstructive response. Histology and HRCT analysis suggest a positive application of swine as a model for a bleomicine inducing of fibrotic interstitial pulmonary disease.

Experimental Actinobacillus pleuropneumoniae challenge in swine: Comparison of computed tomographic and radiographic findings during disease

“…The CT protocol used, based on 7 mm slices and a reconstruction interval of 5 mm was found appropriate for the detection of macroscopic lung lesions attributed to App . The detection of microscopic lung changes such as interstitial pneumonia (which may be a finding in viral pneumonias) is expected to require slices as narrow as 1 mm [29,30]. These conditions could not be achieved with the type of CT equipment and the short examination times used in our study.…”

Pseudomonas aeruginosa toxins

“…The goal was to create a strain of mice deficient in CFTR or with mutant CFTR that would spontaneously develop P. aeruginosa infection, or at least maintain a chronic infection without the use of agar beads. Numerous models were developed, reflecting the wide variety of known disease-causing mutations in human cftr (reviewed in [159,160]). Although most mouse models appear to mimic human CF gastrointestinal disease, none fully replicate human CF lung disease in terms of abnormal ion secretion, chronic inflammation, or spontaneous infection.…”

Stem Cells, Cell Therapies, and Bioengineering in Lung Biology and Diseases. Comprehensive Review of the Recent Literature 2010–2012

“…These include human amniotic fluid or amnion-derived epithelial cells (181)(182)(183)(184)(185)(186)(187)(188)(189)(190)(191)(192)(193)(194)(195)(196). A growing body of literature also describes the effectiveness of a heterogenous population of BM-derived mononuclear cells in ameliorating injury in mouse models of ALI, allergic airways inflammation, COPD, hemorrhagic shock, sepsis, and silicosis (310)(311)(312)(313)(314)(315)(316)(317)(318)(319). The BM-derived mononuclear cell fraction does contain MSCs, and it is not clear whether it is the MSC fraction that is responsible for the observed effects.…”