Cell therapy for spinal cord regeneration

Willerth, Stephanie M.; Sakiyama‐Elbert, Shelly E.

doi:10.1016/j.addr.2007.08.028

Cited by 98 publications

(81 citation statements)

References 123 publications

(157 reference statements)

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“…A variety of new cell injection protocols are currently under investigation for treatment of a myriad of diseases and injuries (17,(31)(32)(33)(34). Here, we focus on potential applications in the central nervous system.…”

mentioning

confidence: 99%

Two-component protein-engineered physical hydrogels for cell encapsulation

Foo

Lee

Mulyasasmita

et al. 2009

Proc. Natl. Acad. Sci. U.S.A.

293

255

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Current protocols to encapsulate cells within physical hydrogels require substantial changes in environmental conditions (pH, temperature, or ionic strength) to initiate gelation. These conditions can be detrimental to cells and are often difficult to reproduce, therefore complicating their use in clinical settings. We report the development of a two-component, molecular-recognition gelation strategy that enables cell encapsulation without environmental triggers. Instead, the two components, which contain multiple repeats of WW and proline-rich peptide domains, undergo a sol-gel phase transition upon simple mixing and hetero-assembly of the peptide domains. We term these materials mixing-induced, two-component hydrogels. Our results demonstrate use of the WW and proline-rich domains in protein-engineered materials and expand the library of peptides successfully designed into engineered proteins. Because both of these association domains are normally found intracellularly, their molecular recognition is not disrupted by the presence of additional biomolecules in the extracellular milieu, thereby enabling reproducible encapsulation of multiple cell types, including PC-12 neuronal-like cells, human umbilical vein endothelial cells, and murine adult neural stem cells. Precise variations in the molecular-level design of the two components including (i) the frequency of repeated association domains per chain and (ii) the association energy between domains enable tailoring of the hydrogel viscoelasticity to achieve plateau shear moduli ranging from Ϸ9 to 50 Pa. Because of the transient physical crosslinks that form between association domains, these hydrogels are shear-thinning, injectable, and self-healing. Neural stem cells encapsulated in the hydrogels form stable three-dimensional cultures that continue to self-renew, differentiate, and sprout extended neurites.biomaterial ͉ cell transplantation ͉ protein engineering

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mentioning

confidence: 99%

Two-component protein-engineered physical hydrogels for cell encapsulation

Foo

Lee

Mulyasasmita

et al. 2009

Proc. Natl. Acad. Sci. U.S.A.

293

255

View full text Add to dashboard Cite

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“…For CNS poly(lactic acid) (PLA)/poly(glycolic acid) (PGA)/polyinjuries regeneration is not just replacement but re-growth of the lost neuronal circuitry, followed by pro-MATERIALS AND METHODS motion of plasticity of the spared and regenerated neuPreparation of the SAPNS Solution rons (9,56).…”

Section: Synthetic Materialsmentioning

confidence: 99%

“…on serum or feeder cell layers. There is a need for the development of culture methods for human stem cells PC12 Cell Culture that involve chemically defined media (56). The end PC12 cells were purchased and plated in 10-cm polygoal for cellular therapies would be to create cell lines styrene dishes, grown in a mixture of Hams F12k mefor transplantation that do not require immune suppresdium with 10% horse serum and 2.5% fetal bovine sesion of the patient (56).…”

Section: Synthetic Materialsmentioning

confidence: 99%

“…oped for controlling the release of drugs from scaffolds, there are still challenges to be addressed for these scafThe successful storage and implantation of stem cells poses significant challenges for tissue engineering in the folds to serve as successful treatments (23,45,56,59,60):…”

Section: Introductionmentioning

confidence: 99%

“…In treating TBI or SCI preimpregnated with cells (36); the entire scaffold is then implanted in the damaged tissue area (16). drug-delivering scaffolds may need to be combined with cell transplantation to obtain functional recovery (56).…”

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Forever Young: How to Control the Elongation, Differentiation, and Proliferation of Cells Using Nanotechnology

et al. 2009

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Within the emerging field of stem cells there is a need for an environment that can regulate cell activity, to slow down differentiation or proliferation, in vitro or in vivo while remaining invisible to the immune system. By creating a nanoenvironment surrounding PC12 cells, Schwann cells, and neural precursor cells (NPCs), we were able to control the proliferation, elongation, differentiation, and maturation in vitro. We extended the method, using self-assembling nanofiber scaffold (SAPNS), to living animals with implants in the brain and spinal cord. Here we show that when cells are placed in a defined system we can delay their proliferation, differentiation, and maturation depending on the density of the cell population, density of the matrix, and the local environment. A combination of SAPNS and young cells can be implanted into the central nervous system (CNS), eliminating the need for immunosuppressants.

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Minimally Invasive and Regenerative Therapeutics

et al. 2018

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Advances in biomaterial synthesis and fabrication, stem cell biology, bioimaging, microsurgery procedures, and microscale technologies have made minimally invasive therapeutics a viable tool in regenerative medicine. Therapeutics, herein defined as cells, biomaterials, biomolecules, and their combinations, can be delivered in a minimally invasive way to regenerate different tissues in the body, such as bone, cartilage, pancreas, cardiac, skeletal muscle, liver, skin, and neural tissues. Sophisticated methods of tracking, sensing, and stimulation of therapeutics in vivo using nanobiomaterials and soft bioelectronic devices provide great opportunities to further develop minimally invasive and regenerative therapeutics (MIRET). In general, minimally invasive delivery methods offer high yield with low risk of complications and reduced costs compared to conventional delivery methods. Here, we review minimally invasive approaches for delivering regenerative therapeutics into the body. The use of MIRET to treat different tissues and organs is described. Although some clinical trials have been done using MIRET, it is hoped that such therapeutics find wider applications to treat patients. Finally, we highlight some future perspective and challenges for this emerging field.

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Cell therapy for spinal cord regeneration

Cited by 98 publications

References 123 publications

Two-component protein-engineered physical hydrogels for cell encapsulation

Two-component protein-engineered physical hydrogels for cell encapsulation

Forever Young: How to Control the Elongation, Differentiation, and Proliferation of Cells Using Nanotechnology

Minimally Invasive and Regenerative Therapeutics

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