Cell-to-cell diversification in ERBB-RAS-MAPK signal transduction that produces cell-type specific growth factor responses

Miyagi, Hiraku; Hiroshima, Michio; Sako, Yasushi

doi:10.1016/j.biosystems.2020.104293

Cited by 5 publications

(4 citation statements)

References 25 publications

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“…In our current study, we measured the translocation dynamics of SHC, GRB2, and ERK after HRG stimulation in MCF7 cells to investigate the specificity of SHC and GRB2 functions during the signaling for cell differentiation. HRG is a ligand for ERBB3 and B4, and heterodimerization between the ERBBs induces activation, that is, tyrosine phosphorylation of all four ERBBs ( Miyagi et al. , 2020 ).…”

Section: Discussionmentioning

confidence: 99%

p52Shc regulates the sustainability of ERK activation in a RAF-independent manner

Yoshizawa

Umeki

Yamamoto

et al. 2021

MBoC

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p52SHC (SHC) and GRB2 are adaptor proteins involved in the RAS/MAPK (ERK) pathway mediating signals from cell-surface receptors to various cytoplasmic proteins. To further examine their roles in signal transduction, we studied the translocation of fluorescently-labeled SHC and GRB2 to the cell surface, caused by the activation of ERBB receptors by heregulin (HRG). We simultaneously evaluated activated ERK translocation to the nucleus. Unexpectedly, the translocation dynamics of SHC were sustained when those of GRB2 were transient. The sustained localization of SHC positively correlated with the sustained nuclear localization of ERK, which became more transient after SHC knockdown. SHC-mediated PI3K activation was required to maintain the sustainability of the ERK translocation regulating MEK but not RAF. In cells overexpressing ERBB1, SHC translocation became transient, and the HRG-induced cell fate shifted from a differentiation to a proliferation bias. Our results indicate that SHC and GRB2 functions are not redundant, but that SHC plays the critical role in the temporal regulation of ERK activation.

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Section: Discussionmentioning

confidence: 99%

p52Shc regulates the sustainability of ERK activation in a RAF-independent manner

Yoshizawa

Umeki

Yamamoto

et al. 2021

MBoC

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“…Notch functions as a membrane-bound transcription factor, which can be triggered by ligands, such as Serrate and Delta, whereas the ErbB/MAPK pathway could transduce signals from the cell membrane to the nucleus through cascade amplification to activate required genes. 41,42 Endocytosis, which transports extracellular materials into the cell through membrane deformation, plays an important role in Notch-mediated transcription, whereas the protein export involves posttranslational translocation, ribosome binding, as well as transmembrane transport. 43 Hence, we speculated that acute exposure to DBDPE altered the methylation status of Notchmediated signaling pathway and led to transcriptional changes of genes involved in ribosome biogenesis and phototransduction in F0 zebrafish larvae.…”

Section: Dbdpe Induced Multi-and Transgenerationalmentioning

confidence: 99%

Multi- and Transgenerational Developmental Impairments Are Induced by Decabromodiphenyl Ethane (DBDPE) in Zebrafish Larvae

Sun

Zhou

Zhu

et al. 2023

Environ. Sci. Technol.

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A novel brominated flame retardant decabromodiphenyl ethane (DBDPE) has become a ubiquitous emerging pollutant; hence, the knowledge of its long-term toxic effects and underlying mechanism would be critical for further health risk assessment. In the present study, the multi-and transgenerational toxicity of DBDPE was investigated in zebrafish upon a life cycle exposure at environmentally relevant concentrations. The significantly increased malformation rate and declined survival rate specifically occurred in unexposed F2 larvae suggested transgenerational development toxicity by DBDPE. The changing profiles revealed by transcriptome and DNA methylome confirmed an increased susceptibility in F2 larvae and figured out potential disruptions of glycolipid metabolism, mitochondrial energy metabolism, and neurodevelopment. The changes of biochemical indicators such as ATP production confirmed a disturbance in the energy metabolism, whereas the alterations of neurotransmitter contents and light−dark stimulated behavior provided further evidence for multi-and transgenerational neurotoxicity in zebrafish. Our findings also highlighted the necessity for considering the long-term impacts when evaluating the health of wild animals as well as human beings by emerging pollutants.

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“…The ErbB signaling pathway is a complex network that regulates various cellular processes, including growth, differentiation, survival and migration. One of its components, the ErbB-1 receptor, has the ability to modulate the transcription and expression of genes involved in inflammation via the MAPK pathway [43]. The MAPK pathway includes three subgroups (ERK, p38, and JNK) [44], which are called "upstream milestones" in inflammation and apoptosis.…”

Section: Discussionmentioning

confidence: 99%

Structural Characterization and Anti-Inflammatory Activity of Polysaccharides from Tremella fuciformis on Monosodium Urate-Stimulated RAW264.7 Macrophages

Deng,

Wu,

Xiao

et al. 2023

Foods

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The structural characteristics and anti-inflammatory activity of Tremella fuciformis polysaccharides (TFPs) were investigated. The study showed that TFPs were mainly composed of mannose, rhamnose, glucuronic acid, glucose, galactose, xylose, and fucose. TFPs significantly inhibited monosodium urate (MSU)-induced inflammation of RAW264.7 cells, as well as the secretion levels of TNF-α, IL-1β, and IL-18 cytokines. The concentrations of malondialdehyde and reactive oxygen species in RAW264.7 macrophages were reduced, but superoxide dismutase activity was increased. RNA-Seq technology was applied to explore the mechanisms of TFPs ameliorating MSU-induced inflammation of RAW264.7 macrophages. Results revealed that TFPs significantly reduce MSU-stimulated inflammatory damage in RAW 264.7 cells by inhibiting signaling pathways like the hypoxia inducible factor-1 (HIF-1) signaling pathway and erythroblastic oncogene B (ErbB) signaling pathway. This study provides a foundation for TFPs to be developed as novel anti-inflammatory drugs.

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Cell-to-cell diversification in ERBB-RAS-MAPK signal transduction that produces cell-type specific growth factor responses

Cited by 5 publications

References 25 publications

p52Shc regulates the sustainability of ERK activation in a RAF-independent manner

p52Shc regulates the sustainability of ERK activation in a RAF-independent manner

Multi- and Transgenerational Developmental Impairments Are Induced by Decabromodiphenyl Ethane (DBDPE) in Zebrafish Larvae

Structural Characterization and Anti-Inflammatory Activity of Polysaccharides from Tremella fuciformis on Monosodium Urate-Stimulated RAW264.7 Macrophages

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