Cell toxicity of superparamagnetic iron oxide nanoparticles

Mahmoudi, Morteza; Simchi, A.; Milani, Abbas S.; Stroeve, Pieter

doi:10.1016/j.jcis.2009.04.046

Cited by 350 publications

(246 citation statements)

References 35 publications

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“…Thus, rendering multicellular spheroids with magnetic functionality is deemed important for assembly of artificial tissues, and magnetic nanoparticles have been introduced into cells prior to fabrication of spheroids [9]. Importantly, PAH-stabilized MNPs are equally applicable to both primary and malignant cells, whereas earlier reports suggest different ways to functionalize cancer and normal cells [45,46]. Magnetic functionality in spherical tissue engineering blocks is pivotal for the effective positioning and retention of spheroids at desired areas during tissue build-up.…”

Section: Fabrication Of Layered Planar Tissue Constructs and Multicelmentioning

confidence: 99%

Cell surface engineering with polyelectrolyte-stabilized magnetic nanoparticles: A facile approach for fabrication of artificial multicellular tissue-mimicking clusters

et al. 2015

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Regenerative medicine requires new ways to assemble and manipulate cells for fabrication of tissue-like constructs. Here we report a novel approach for cell surface engineering of human cells using polymer-stabilized magnetic nanoparticles (MNPs). Cationic polyelectrolyte-coated MNPs are directly deposited onto cellular membranes, producing a mesoporous semi-permeable layer and rendering cells magnetically responsive. Deposition of MNPs can be completed within minutes, under cell-friendly conditions (room temperature and physiologic media). Microscopy (TEM, SEM, AFM, and enhanced dark-field imaging) revealed the intercalation of nanoparticles into the cellular microvilli network. A detailed viability investigation was performed and suggested that MNPs do not inhibit membrane integrity, enzymatic activity, adhesion, proliferation, or cytoskeleton formation, and do not induce apoptosis in either cancer or primary cells. Finally, magnetically functionalized cells were employed to fabricate viable layered planar (two-cell layers) cell sheets and 3D multicellular spheroids.

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Section: Fabrication Of Layered Planar Tissue Constructs and Multicelmentioning

confidence: 99%

Cell surface engineering with polyelectrolyte-stabilized magnetic nanoparticles: A facile approach for fabrication of artificial multicellular tissue-mimicking clusters

et al. 2015

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“…FT-IR was used to check whether the reagent was bound to the surface of the particles, in a wavenumber range 4000-600 cm -1 [24,25]. TGA analysis was carried out with heating rate of 10 ºC/min in nitrogen until 900 ºC [26,27].…”

Section: Synthesis Of Adsorbent Materialsmentioning

confidence: 99%

Arsenate removal with 3-mercaptopropanoic acid-coated superparamagnetic iron oxide nanoparticles

Morillo

Uheida

Pérez

et al. 2015

Journal of Colloid and Interface Science

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In the present work, superparamagnetic iron oxide nanoparticles (SPION) surface-coated with 3-mercaptopropanoic acid (3-MPA) were prepared and their feasibility for the removal of arsenate from dilute aqueous solutions was demonstrated. The synthesized 3-MPA-coated SPION was characterized using transmission electron microscopy (TEM), thermogravimetric analysis (TGA) and Fourier transform infra-red spectrometry (FTIR). Separation efficiency of the coated nanoparticles and the equilibrium isotherm of arsenate adsorption were investigated. The obtained results reveal the arsenate adsorption to be highly pH-dependent, and the maximum adsorption was attained in less than 60 minutes. The resulting increase of 3-MPA-coated SPION adsorption capacity to twice the adsorption capacity of SPION alone under the same conditions is attributed to the increase of active adsorption sites. An adsorption reaction is proposed. On the other hand, efficient recovery of arsenate from the loaded nanoparticles was achieved using nitric acid (HNO 3 ) solution, which also provides a concentration over the original arsenate solution. HIGHLIGHTS• Feasibility of using SPION modified with 3MPA for the removal of As(V).• The adsorbent system results in an effective sorption for selective As(V) removal.• The adsorption capacity for As(V) is higher comparing with other adsorbent systems.• Adsorption mechanism has been proposed as the most suitable considering the results.

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“…In order to obtain the single coated NPs, the dextran/iron mass ratio was xed at 2. 29 The SPIONs were formed by dropwise addition of a base medium (i.e. NaOH) into the prepared dextran and iron salt mixtures with vigorous stirring under argon protection.…”

Section: Synthesis Of Ultra-small Spions With Various Surface Charactmentioning

confidence: 99%

Protein corona affects the relaxivity and MRI contrast efficiency of magnetic nanoparticles

et al. 2013

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Magnetic nanoparticles (NPs) are increasingly being considered for use in biomedical applications such as biosensors, imaging contrast agents and drug delivery vehicles. In a biological fluid, proteins associate in a preferential manner with NPs. The small sizes and high curvature angles of NPs influence the types and amounts of proteins present on their surfaces. This differential display of proteins bound to the surface of NPs can influence the tissue distribution, cellular uptake and biological effects of NPs. To date, the effects of adsorption of a protein corona (PC) on the magnetic properties of NPs have not been considered, despite the fact that some of their potential applications require their use in human blood.Here, to investigate the effects of a PC (using fetal bovine serum) on the MRI contrast efficiency of superparamagnetic iron oxide NPs (SPIONs), we have synthesized two series of SPIONs with variation in the thickness and functional groups (i.e. surface charges) of the dextran surface coating. We have observed that different physico-chemical characteristics of the dextran coatings on the SPIONs lead to the formation of PCs of different compositions. 1 H relaxometry was used to obtain the longitudinal, r 1 , and transverse, r 2 , relaxivities of the SPIONs without and with a PC, as a function of the Larmor frequency. The transverse relaxivity, which determines the efficiency of negative contrast agents (CAs), is very much dependent on the functional group and the surface charge of the SPIONs' coating. The presence of the PC did not alter the relaxivity of plain SPIONs, while it slightly increased the relaxivity of the negatively charged SPIONs and dramatically decreased the relaxivity of the positively charged ones, which was coupled with particle agglomeration in the presence of the proteins. To confirm the effect of the PC on the MRI contrast efficiency, in vitro MRI experiments at n ¼ 8.5 MHz were performed using a low-field MRI scanner. The MRI contrasts, produced by different samples, were fully in agreement with the relaxometry findings.It is well-recognized that the surface of nanoparticles (NPs) is covered by biomolecules (proteins, sugars and lipids) upon coming into contact with biological systems, resulting in the formation of a protein "corona" that is strongly associated with the NPs' surface and that denes how living organisms (e.g. cells) "see" the NPs in a biological milieu. 1-5 According to previous reports, the cell "sees" a nano-system in which the NPs are covered by a "hard" corona of slowly exchanging proteins with a surrounding "so" corona consisting of weakly interacting and rapidly exchanging proteins. 6 The composition of the protein corona (PC) is highly dependent on the physicochemical properties of the NPs such as their size, composition, and surface characteristics. 7 Superparamagnetic iron oxide nanoparticles (SPIONs) have been recognized as very promising nanosystems with good biocompatibility 8,9 and high potential to be used for diagnosis and therapy simultaneou...

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Cell toxicity of superparamagnetic iron oxide nanoparticles

Cited by 350 publications

References 35 publications

Cell surface engineering with polyelectrolyte-stabilized magnetic nanoparticles: A facile approach for fabrication of artificial multicellular tissue-mimicking clusters

Cell surface engineering with polyelectrolyte-stabilized magnetic nanoparticles: A facile approach for fabrication of artificial multicellular tissue-mimicking clusters

Arsenate removal with 3-mercaptopropanoic acid-coated superparamagnetic iron oxide nanoparticles

Protein corona affects the relaxivity and MRI contrast efficiency of magnetic nanoparticles

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