Cell tracking using multimodal imaging

Srinivas, Mangala; Melero, Ignacio; Kaempgen, Eckhart; Figdor, Carl G.; Vries, I. Jolanda M. de

doi:10.1002/cmmi.1561

Cited by 21 publications

(18 citation statements)

References 35 publications

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“…5 Copyright: American Scientific Publishers enormous attention due to its analytic and diagnostic ability at the molecular or cellular level. 13 As a result, a new discipline, known as "molecular imaging," 14 which combines molecular biology and in vivo imaging, has emerged. Representative imaging platforms include computed X-ray tomography (CT), 15 optical tomography (DOT), 16 a noninvasive emerging imaging modality, that makes use of the light in the near-infrared spectrum, magnetic resonance imaging (MRI), 17 positron emission tomography (PET), 18 single-photon-emission computed tomography (SPECT), 19 and ultrasound, 20 as well as combinations of the same.…”

Section: Introductionmentioning

confidence: 99%

Red Blood Cells as Carriers of Iron Oxide-Based Contrast Agents for Diagnostic Applications

Antonelli¹,

Magnani²

2014

j biomed nanotechnol

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New biomedical strategies are being developed to improve disease detection, therapeutic monitoring, and treatment efficacy in several contexts for example in anti-angiogenic chemotherapy, and prevention of cardiovascular disorders. The use of contrast agents to improve the sensitivity and resolution of diagnostic imaging modalities, detect lesions or organs, and evaluate organ function was immediately recognized. In the last years, superparamagnetic iron oxide (SPIO) nanoparticles have been clinically used as MRI contrast agents for the diagnosis of liver diseases, due to their ability to shorten T 2 * relaxation times. After the intravenous administration, SPIO nanoparticles are rapidly eliminated from the bloodstream since they are selectively taken up by the Kupffer cells in the liver, spleen, and bone marrow thus improving the clinical tumor detection in these tissues. However, for the investigation of vascular system including functional cardiac diagnosis, angiography and cardiac wall motion assessment, contrast agents with long blood circulation time would be necessary. In fact, imaging applications for long-term monitoring require the repeated administration of SPIO bolus injections, which complicates quantitative comparisons due to the temporal variations in concentration. In this context, researchers have developed several SPIO delivery systems based on red blood cells (RBCs) used as carriers to increase the blood circulation time of contrast agents. Encapsulation of SPIOs into RBCs appears the most attractive strategy to obtain magnetic RBC-constructs suitable for MRI and MPI analyses of blood vascular system.

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Section: Introductionmentioning

confidence: 99%

Red Blood Cells as Carriers of Iron Oxide-Based Contrast Agents for Diagnostic Applications

Antonelli¹,

Magnani²

2014

j biomed nanotechnol

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“…The evaluation of their homing capacities was carried out by bioluminescence imaging method (BLI). As an emerging in vivo cell tracing method, BLI has the advantages of high specificity and sensitivity and most importantly, it can visualize cells’ dynamic migrating process by successive imaging1415. Thus, it could provide us more detailed and objective information about DCs’ homing process before and after cryopreservation.…”

mentioning

confidence: 99%

Mature dendritic cell derived from cryopreserved immature dendritic cell shows impaired homing ability and reduced anti-viral therapeutic effects

Zhou

Zhao

Wang

et al. 2016

Sci Rep

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Cryopreservation is critical in reducing redundant operations and also in quality control in dendritic cell (DC) therapy. Full maturation and efficient homing of DCs to T cell-region constitute a crucial aspect of DC immunotherapy; however, the in vivo migration and distribution pattern, as well as the anti-viral effect of DCs that matured from cryopreserved immature DCs (cryoim-mDCs) remain to be revealed. In the present study, we compared cryoim-mDCs with DCs matured from fresh immature DCs (fmDCs) in the aspects of phenotypes, in vivo homing capacities as well as the anti-viral therapeutic effects to further clarify the effect of cryopreservation on DC-based cytotherapy. The results showed that cryopreservation impaired the homing ability of DCs which was associated with the reduced expression of CCR7 and disturbed cytoskeleton arrangement. Moreover, the antigen-specific CD8+ T cell response induced by cryoim-mDCs was much weaker than that induced by fmDCs in both the spleen and liver draining lymph nodes, which provided reduced protection from viral invasions. In conclusion, cryopreservation is a good method to keep the viability of immature DCs, however, the in vivo homing capacity and anti-viral therapeutic effect of DCs matured from frozen immature DCs were hindered to some extent.

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“…Multimodal imaging allows the collection of complementary information from a single experiment. Therefore, it permits taking advantage of a technique's specific strengths, avoiding individual disadvantages, and thus improving single monitoring techniques (De Vocht et al 2012;Srinivas et al 2013;Tang et al 2015;Wolfs et al 2014). BLI, PET, and SPECT show higher sensitivities but lower resolutions than MRI.…”

Section: Multimodal Imagingmentioning

confidence: 98%

“…This can be achieved by applying either one CA that can be detected by different imaging modalities or a combination of different labeling agents (Srinivas et al 2013). Multimodal imaging allows the collection of complementary information from a single experiment.…”

Section: Multimodal Imagingmentioning

confidence: 99%

Lost signature: progress and failures in in vivo tracking of implanted stem cells

Haar

Lavrentieva

Stahl

et al. 2015

Appl Microbiol Biotechnol

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Stem cell therapy as a part of regenerative medicine provides promising approaches for the treatment of injuries and diseases. The increasing use of mesenchymal stem cells in various medical treatments created the demand for long-term in vivo cell tracking methods. Therefore, it is necessary to analyze post-transplantational survival, biodistribution, and engraftment of cells. Furthermore, stem cell treatment has been discussed controversially due to possible association with tumor formation in the recipient. For therapeutic purpose, stem cells must undergo substantial manipulation such as differentiation and in vitro expansion, and this can lead to the occurrence of genetic aberrations and altered expression of both tumor suppression and carcinogenic factors. To control therapy, it is necessary to find a reliable and general method to track and identify implanted cells in the recipient. This is especially challenging for autologous transplantations, as standard fingerprinting methods cannot be applied. An optimal technique for in vivo cell monitoring does not yet exist, and its development holds several challenges: small numbers of transplanted cells, possibility of cell number quantification, minimal transfer of the contrast agent to non-transplanted cells, and no genetic modification. This review discusses most of the proposed solutions, including magnetic resonance imaging, magnetic particle imaging, positron emission tomography, single-photon emission computed tomography, and optical imaging methods. Additionally, the recent research on cell labeling for stem cell monitoring after transplantation including in vitro, ex vivo, and in vivo imaging studies is described. Promising future imaging modalities for stem cell monitoring after transplantation are shown.

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Cell tracking using multimodal imaging

Cited by 21 publications

References 35 publications

Red Blood Cells as Carriers of Iron Oxide-Based Contrast Agents for Diagnostic Applications

Red Blood Cells as Carriers of Iron Oxide-Based Contrast Agents for Diagnostic Applications

Mature dendritic cell derived from cryopreserved immature dendritic cell shows impaired homing ability and reduced anti-viral therapeutic effects

Lost signature: progress and failures in in vivo tracking of implanted stem cells

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