Cell transfection
            <i>in vitro</i>
            and
            <i>in vivo</i>
            with nontoxic TAT peptide-liposome–DNA complexes

Torchilin, Vladimir P.; Levchenko, Tatyana; Rammohan, Ram; Володина, Наталья; Papahadjopoulos‐Sternberg, Brigitte; D’Souza, Gerard G. M.

doi:10.1073/pnas.0435906100

Cited by 422 publications

(266 citation statements)

References 47 publications

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“…It was also reported that Tat-carrying [46] and protamine-carrying [44] iron nanoparticles were readily internalised by cells. Moreover, Tat-liposomes of 200 nm showed potential for intracellular gene delivery in vitro and in vivo [47]and [48]. These experiments suggest that the improved immunological performance of protamine-coated antigen-containing PLGA microparticles was mediated by the cell-penetrating properties of the arginine-rich protamine.…”

Section: Discussionmentioning

confidence: 82%

Surface coating of PLGA microparticles with protamine enhances their immunological performance through facilitated phagocytosis

Gómez

Csaba

Fischer

et al. 2008

Journal of Controlled Release

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Surface modifications of poly(lactide-co-glycolide) microparticles with different polycationic electrolytes have mainly been studied for conjugation to antigens and/or adjuvants. However, the in vivo immunological effects of using surface charged particles have not been address yet. In this study, microparticles were coated or not with protamine, a cationic and arginine-rich electrolyte that confers microparticles with a positively surface charge. We then evaluated the potential of protamine-coatings to assist the induction of immune responses in mice. Interestingly, enhanced antibodies and T-cell responses were observed in mice treated with the coated particles. In vitro studies suggested that the improved immunological performance was mediated by an increased uptake. Indeed, protamine-coated particles that carried a plasmid were even internalised into non-phagocytic cells and to cause their transfection. These results open the way for further research into a novel technology that combines the use protamine for facilitated cell penetration of that and biodegradable microparticles for prolonged antigen or drug release.

show abstract

Section: Discussionmentioning

confidence: 82%

Surface coating of PLGA microparticles with protamine enhances their immunological performance through facilitated phagocytosis

Gómez

Csaba

Fischer

et al. 2008

Journal of Controlled Release

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“…Several studies have indicated that particles of different sizes could enter cells via different mechanisms. 8,83,85,86 Vesicles in clathrin-and caveolin-mediated endocytosis are small in size (<100 nm), which can significantly limit their transport capacities for pDNA with a similar size. In contrast, macropinocytosis, which is known to be responsible for cellular uptake of viruses, 87,88 nanoparticles, 89 and naked pDNA, 90 can generate vesicles with sizes of up to 5 mm that could transport a significantly larger amount of pDNA per vesicle.…”

Section: Discussionmentioning

confidence: 99%

Involvement of a Rac1-Dependent Macropinocytosis Pathway in Plasmid DNA Delivery by Electrotransfection

et al. 2017

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“…4). 2,[93][94][95] Two of the most studied CPPs are the transactivating transcriptional activator (TAT) of HIV-1 virus 96,97 and the penetratin peptide of the Antennapedia homeodomain of Drosophilia. 98 Other protein-derived or chemically synthesized CPPs such as polyarginines have also been characterized.…”

Section: Biological Activitiesmentioning

confidence: 99%

Structure and dynamics of cationic membrane peptides and proteins: Insights from solid‐state NMR

Hong¹,

Su²

2011

Protein Science

130

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Many membrane peptides and protein domains contain functionally important cationic Arg and Lys residues, whose insertion into the hydrophobic interior of the lipid bilayer encounters significant energy barriers. To understand how these cationic molecules overcome the free energy barrier to insert into the lipid membrane, we have used solid-state NMR spectroscopy to determine the membrane-bound topology of these peptides. A versatile array of solid-state NMR experiments now readily yields the conformation, dynamics, orientation, depth of insertion, and site-specific protein-lipid interactions of these molecules. We summarize key findings of several Arg-rich membrane peptides, including b-sheet antimicrobial peptides, unstructured cell-penetrating peptides, and the voltage-sensing helix of voltage-gated potassium channels. Our results indicate the central role of guanidinium-phosphate and guanidinium-water interactions in dictating the structural topology of these cationic molecules in the lipid membrane, which in turn account for the mechanisms of this functionally diverse class of membrane peptides.

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Cell transfection in vitro and in vivo with nontoxic TAT peptide-liposome–DNA complexes

Cited by 422 publications

References 47 publications

Surface coating of PLGA microparticles with protamine enhances their immunological performance through facilitated phagocytosis

Surface coating of PLGA microparticles with protamine enhances their immunological performance through facilitated phagocytosis

Involvement of a Rac1-Dependent Macropinocytosis Pathway in Plasmid DNA Delivery by Electrotransfection

Structure and dynamics of cationic membrane peptides and proteins: Insights from solid‐state NMR

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