Cell transfection using layer-by-layer (LbL) coated calixarene-based solid lipid nanoparticles (SLNs)

Abstract

Polycationic calixarene-based solid lipid nanoparticles (SLNs) have been loaded at their surface with DNA and chitosan and studied for their ability to transfect mammalian cells.

Solid Lipid Nanoparticle-Based Calix[n]arenes and Calix-Resorcinarenes as Building Blocks: Synthesis, Formulation and Characterization

“…The red color representing the labeled-chitosan appeared to be in the cytoplasmic compartment of the cell [98]. …”

“…Due to their cationic nature, the SLNs based on 17 were capable of complexing DNA [ 91 – 96 ]. Aiming to open new prospects to use these SLNs as gene delivery systems [ 97 ], cell transfection using a layer by layer (LbL) coating technique was investigated, and the SLNs were loaded at their surface with DNA and chitosan [ 98 ]. Chitosan is a biopolymer capable of adhering to negatively charged surfaces such as skin and mucosa and possessing wound healing proprieties [ 99 ].…”

Elucidating the mechanism of the considerable mechanical stiffening of DNA induced by the couple Zn2+/Calix[4]arene-1,3-O-diphosphorous acid

“…Artificial DNA binders present a great interest in the biomedical field 4 . They can be used in various ways, such as the transportation of DNA through the cell membrane in transfection or gene therapy 5 for blocking a specific DNA sequence to avoid the transcription of a specific gene 6 or arresting DNA replication in cancer treatment 7 .…”

Cation binding resorcinarene bis-crowns: the effect of lower rim alkyl chain length on crystal packing and solid lipid nanoparticles

“…Since calixarenes and resorcinarenes are usually solid materials at room temperature, they can be used for preparation of solid lipid nanoparticles and studies of their potential in encapsulation of biologically important guests, DNA 60 for cell transfection and surface modification for drug targeting have been published. [14][15][16][17] Calixarenes and resorcinarenes with crown ether bridges connecting the hydroxyl groups are very selective cation receptors called calixcrowns. 18 Depending on the number of 65 oxygen donors and thus the length and geometry of the crown bridge, calixcrowns have very good affinity towards alkali and alkaline earth metal cations and ammonium ions.…”