Cell turnover in neuromasts of zebrafish larvae

Williams, Jennifer A. E.; Holder, Nigel

doi:10.1016/s0378-5955(00)00039-3

Cited by 226 publications

(247 citation statements)

References 42 publications

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“…In fishes and amphibians, hair cells are renewed continuously during embryogenesis and postembryonic life. Hair cells located in the centre of the neuromast that are lost by programmed cell death are replaced in the juvenile and the adult by new hair cells generated from supporting cells (Williams and Holder, 2000). Understanding the molecular mechanisms that regulate the precursor cells to become mature hair cells in zebrafish could be used to devise therapies to replace sensory cells in the human inner ear to correct deafness.…”

Section: Pc5 Expression Within the Mouse Newborn Inner Earmentioning

confidence: 99%

Molecular cloning and embryonic expression of zebrafish PCSK5 co‐orthologues: Functional assessment during lateral line development

Chitramuthu

Baranowski

Cadieux

et al. 2010

Developmental Dynamics

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Pro-protein convertase subtilisin/kexin 5 (PC5, also known as PC6) is a member of the subtilisin-like superfamily of serine proteases implicated in the maturation of latent precursor proteins into their functionally active derivatives. To investigate the functional roles, we have cloned the cDNA sequences encoding two candidate zebrafish PC5 convertases (designated as PCSK5.1 and PCSK5.2) co-orthologous to the single PC5 encoding gene (PCSK5) found in mammals. Both display syntenic correspondence to the human PCSK5 gene. Overall gene architecture has been conserved across species. While PC5.1 mRNA expression is very discrete within the otic vesicle and lateral line neuromasts, PC5.2 transcripts are more ubiquitously expressed within the central nervous system together with specific localization in various organs including liver, intestine, and otic vesicle. Zebrafish PC5.1-deficient embryos display abnormal neuromast deposition within the lateral line system and lack a normal touch response, consistent with the known sensory role that the lateral line plays in spatial awareness and sensing the environment.

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Section: Pc5 Expression Within the Mouse Newborn Inner Earmentioning

confidence: 99%

Molecular cloning and embryonic expression of zebrafish PCSK5 co‐orthologues: Functional assessment during lateral line development

Chitramuthu

Baranowski

Cadieux

et al. 2010

Developmental Dynamics

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“…30 In light of the observed expression patterns, it is interesting to note that zebrafish hair cells undergo constant, apoptosis-mediated turnover. 31 We detected expression of several zebrafish TNF superfamily ligands and receptors in the developing central nervous embryos stained by whole-mount ISH (purple) for zDL1a, zHDR, zOTR, and zTNFR1. zHDR is not detected in the body at this stage and serves as a background control.…”

Section: Additional Sites Of Embryonic Expressionmentioning

confidence: 99%

Delineation of the cell-extrinsic apoptosis pathway in the zebrafish

et al. 2006

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The mammalian extrinsic apoptosis pathway is triggered by Fas ligand (FasL) and Apo2 ligand/tumor necrosis factor (TNF)-related apoptosis-inducing ligand (Apo2L/TRAIL). Ligand binding to cognate receptors activates initiator caspases directly in a death-inducing signaling complex. In Drosophila, TNF ligand binding activates initiator caspases indirectly, through JNK. We characterized the extrinsic pathway in zebrafish to determine how it operates in a nonmammalian vertebrate. We identified homologs of FasL and Apo2L/TRAIL, their receptors, and other components of the cell death machinery. Studies with three Apo2L/TRAIL homologs demonstrated that they bind the receptors zHDR (previously linked to hematopoiesis) and ovarian TNFR (zOTR). Ectopic expression of these ligands during embryogenesis induced apoptosis in erythroblasts and notochord cells. Inhibition of zHDR, zOTR, the adaptor zFADD, or caspase-8-like proteases blocked ligand-induced apoptosis, as did antiapoptotic Bcl-2 family members. Thus, the extrinsic apoptosis pathway in zebrafish closely resembles its mammalian counterpart and cooperates with the intrinsic pathway to trigger tissue-specific apoptosis during embryogenesis in response to ectopic Apo2L/TRAIL expression.

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“…Several possible functions for this are proposed by the authors, including refinement of the hair cell/supporting cell pattern, elimination of inappropriately innervated hair cells, or generation of pathways for ingrowing nerve fibres. Note that in zebrafish lateral line neuromasts, there appears to be a rapid and constant turnover of hair cells, where dying cells at the centre of the neuromast are replaced by mantle cell proliferation at the edge (Williams and Holder, 2000, and see the Growth and Regeneration of Sensory Epithelia section below). Haddon et al (1999) describe numerous TUNELlabelled cells in the region of the developing statoacoustic ganglion in otherwise phenotypically normal siblings of mind bomb mutants at 36 hpf, but it is possible and even likely that some of these represented false positives, as no concomitant changes in nuclear morphology were seen.…”

Section: Cell Death In the Zebrafish Earmentioning

confidence: 99%

Development of the zebrafish inner ear

Whitfield

Riley

Chiang

et al. 2002

Developmental Dynamics

214

186

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Recent years have seen a renaissance of investigation into the mechanisms of inner ear development. Genetic analysis of zebrafish has contributed significantly to this endeavour, with several dramatic advances reported over the past year or two. Here, we review the major findings from recent work in zebrafish. Several cellular and molecular mechanisms have been elucidated, including the signaling pathways controlling induction of the otic placode, morphogenesis and patterning of the otic vesicle, and elaboration of functional attributes of inner ear.

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Cell turnover in neuromasts of zebrafish larvae

Cited by 226 publications

References 42 publications

Molecular cloning and embryonic expression of zebrafish PCSK5 co‐orthologues: Functional assessment during lateral line development

Molecular cloning and embryonic expression of zebrafish PCSK5 co‐orthologues: Functional assessment during lateral line development

Delineation of the cell-extrinsic apoptosis pathway in the zebrafish

Development of the zebrafish inner ear

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