Cell Type-Dependent Specificity and Anti-Inflammatory Effects of Charge-Reversible MSNs-COS-CMC for Targeted Drug Delivery in Cervical Carcinoma

Abstract

The surface charge of nanocarriers inevitably affects drug delivery efficiency; however, the cancer cell specificity, antiinflammatory effects, and charge-reversal points remain to be further addressed in biomedical applications. The aim of this study was to comprehensively assess the cancer cell specificity of DOX-loaded mesoporous silica-chitosan oligosaccharide-carboxymethyl chitosan nanoparticles (DOX@MSNs-COS-CMC) in MCF-7 and HeLa cells, inhibit the production of inflammatory cytokines, and improve the drug accumulation in the tumor site. Intracellular results reveal that the retention time prolonged to 48 h in both HeLa and MCF-7 cells at pH 7.4. However, DOX@MSNs-COS-CMC exhibited a cell type-dependent cytotoxicity and enhanced intracellular uptake in HeLa cells at pH 6.5, due to the clathrin-mediated endocytosis and macropinocytosis in HeLa cells in comparison with the vesicular transport in MCF-7 cells. Moreover, Pearson's correlation coefficient value significantly decreased to 0.25 after 8 h, prompting endosomal escape and drug delivery into the HeLa nucleus. After the treatment of MSNs-COS-CMC at 200 μg/mL, the inflammatory cytokines IL-6 and TNF-α level decreased by 70% and 80%, respectively.

Construction of a Localized and Long-Acting CCN2 Delivery System on Percutaneous Ti Implant Surfaces for Enhanced Soft-Tissue Integration

“…Thus, many designed release systems could only function in the early days of implantation. In the present study, we also used MSNs as the drug carrier for the reason that they possess large surface areas and high pore volumes, resulting in a prolonged drug-release time. , The N 2 adsorption isotherms indicate that MSNs used in this study had a well-defined mesoporous structure with a surface area of 511 m 2 /g and the pore diameter in the range of 3–5 nm (Figure S2). We utilized the soaking method to load CCN2 into MSNs with a drug loading of 1 ng of CCN2 per 3 mg of MSNs.…”

Silicon-containing nanomedicine and biomaterials: materials chemistry, multi-dimensional design, and biomedical application

“…14a). 536 The rationale behind this design is based on the understanding that nanoparticles with a positively charged surface are more likely to adsorb serum proteins to initiate opsonization, further leading to the rapid clearance and sequestration of administered nanocarriers through phagocytosis. PEGylation of nanocarriers has long been considered a universal method to minimize the undesirable MPS clearance by sterically preventing the adsorption of serum proteins.…”

Multifunctional Biopolymers‐Based Composite Materials for Biomedical Applications: A Systematic Review

“…[37] Additionally, polysaccharides could also be used to form efficient drug delivery systems via bring together different drug molecules on to the metal nanoparticles surface. [38] Moreover, these materials have various functional groups, including hydroxyl, amine and carboxyl groups, which guarantee to synthesize a novel biocomposite through easy modification with suitable polymeric materials that sustain preferred properties for use in regenerative medicine and biomedical applications: [39][40][41][42][43] gene transfection and drug delivery, [44][45][46][47][48] wound dressing, [49][50][51] tissue-engineering, [52][53][54] cell based therapeutic application. [55][56][57] Since last few decades, scientists established brilliant biomedical requisitions of metal based nanoparticles, developed via green chemical routes, utilizing variety of biopolymers, such as starch, cellulose, fucoidan, glucan, etc.…”