2024
DOI: 10.1038/s41467-024-46435-0
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Cell type signatures in cell-free DNA fragmentation profiles reveal disease biology

Kate E. Stanley,
Tatjana Jatsenko,
Stefania Tuveri
et al.

Abstract: Circulating cell-free DNA (cfDNA) fragments have characteristics that are specific to the cell types that release them. Current methods for cfDNA deconvolution typically use disease tailored marker selection in a limited number of bulk tissues or cell lines. Here, we utilize single cell transcriptome data as a comprehensive cellular reference set for disease-agnostic cfDNA cell-of-origin analysis. We correlate cfDNA-inferred nucleosome spacing with gene expression to rank the relative contribution of over 490 … Show more

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Cited by 12 publications
(1 citation statement)
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“…In terms of size analysis, cfDNA mostly contained fragments less than 1000 base pairs (bp) ( 6 ). These cfDNA fragments are derived from nuclease-processed nucleosome arrays which consist of 147 bp of DNA wrapped around a histone octamer core with approximately 20-40 bp of unbound linker DNA interspersed ( 7 , 8 ). Short cfDNA fragments(<150 bp) contain a larger fraction of mutated fragments than the total cfDNA pool ( 9 ).…”
Section: Introductionmentioning
confidence: 99%
“…In terms of size analysis, cfDNA mostly contained fragments less than 1000 base pairs (bp) ( 6 ). These cfDNA fragments are derived from nuclease-processed nucleosome arrays which consist of 147 bp of DNA wrapped around a histone octamer core with approximately 20-40 bp of unbound linker DNA interspersed ( 7 , 8 ). Short cfDNA fragments(<150 bp) contain a larger fraction of mutated fragments than the total cfDNA pool ( 9 ).…”
Section: Introductionmentioning
confidence: 99%