2023
DOI: 10.1038/s41477-022-01315-7
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Cell-type-specific PtrWOX4a and PtrVCS2 form a regulatory nexus with a histone modification system for stem cambium development in Populus trichocarpa

Abstract: Stem vascular cambium cells in forest trees produce wood for materials and energy. WOX4 affects the proliferation of such cells in Populus. Here we show that PtrWOX4a is the most highly expressed stem vascular-cambium-specific (VCS) gene in P. trichocarpa, and its expression is controlled by the product of the second most highly expressed VCS gene, PtrVCS2, encoding a zinc finger protein. PtrVCS2 binds to the PtrWOX4a promoter as part of a PtrWOX13a–PtrVCS2–PtrGCN5-1–PtrADA2b-3 protein tetramer. PtrVCS2 preven… Show more

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Cited by 37 publications
(14 citation statements)
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“…Given the high accumulation of auxin in the cambial zone of poplar stems 17,19 , we hypothesized noncellautonomous modulation of cambium-localized auxin signalling impacts wood bre development at the DSX. To test this hypothesis, auxin signalling was attenuated by expressing IAA9m driven by the promoter of poplar WOX4a, a well-known vascular cambium-speci c gene [23][24][25] . The transgenic lines displayed substantial reductions (10.2-34.2%) in plant height, maintained consistent internode counts, and had slightly reduced stem diameters, compared with the WT (Supplementary Fig.…”
Section: Resultsmentioning
confidence: 99%
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“…Given the high accumulation of auxin in the cambial zone of poplar stems 17,19 , we hypothesized noncellautonomous modulation of cambium-localized auxin signalling impacts wood bre development at the DSX. To test this hypothesis, auxin signalling was attenuated by expressing IAA9m driven by the promoter of poplar WOX4a, a well-known vascular cambium-speci c gene [23][24][25] . The transgenic lines displayed substantial reductions (10.2-34.2%) in plant height, maintained consistent internode counts, and had slightly reduced stem diameters, compared with the WT (Supplementary Fig.…”
Section: Resultsmentioning
confidence: 99%
“…Notably, 27 TF genes, including 15 TFs identi ed on the list of vascular cambium-speci c TFs derived from P. trichocarpa cell-type transcriptome analysis, were downregulated by IAA9m overexpression (Extended Data Fig. 2c) 25 . These TFs belong to the AP2, DOF, HD-ZIP, bHLH, and E2F families, encompassing two homologous proteins (termed PLT5a and PLT5b) from the AP2 TF subfamily (Extended Data Fig.…”
Section: Plt5a/b Speci Cally Respond To Auxin Signals In the Vascular...mentioning
confidence: 99%
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“…The pETDuet‐1 vector (71 146; Novagen, Darmstadt, Germany) is used to express target proteins for pull‐down experiments (Dai et al ., 2023). The full‐length coding sequences of PtrWDR5a‐1 , PtrASH2‐2 , PtrRbBP5‐1 , PtrAREB1‐2 , and GFP and C‐terminal coding sequence of PtrSDG2‐1 (amino acids 1559–2030) were cloned into the pETDuet‐1 vector.…”
Section: Methodsmentioning
confidence: 99%
“…For generating overexpression constructs, coding regions of PtrHox2 , PtrHox46 , and PtrHox52 were amplified and cloned into the pBI121 vector. Each plasmid was introduced into Agrobacterium tumefaciens cells (GV3101) and then infected the stem explants of P. trichocarpa (Dai et al ., 2023). RT‐qPCR was performed to detect the expression level of transgenes in the SDX of transgenic plants.…”
Section: Methodsmentioning
confidence: 99%