Cell Type Variability in the Incorporation of Lipids in the Dengue Virus Virion

Abstract

A lipid bilayer produced from the host membrane makes up around 20% of the weight of the dengue virus (DENV) virion and is crucial for virus entry. Despite its significance, the virion’s lipid composition is still poorly understood. In tandem with lipid profiles of the cells utilised to generate the virions, this work determined a partial lipid profile of DENV virions derived from two cell lines (C6/36 and LLC-MK2). The results showed distinctive profiles between the two cell types. In the mammalian LLC-MK2 cells, 30.8% (73/237 identified lipid species; 31 upregulated, 42 downregulated) of lipid species were altered in response to infection, whilst in insect C6/36 cells only 12.0% (25/208; 19 upregulated, 6 downregulated) of lipid species showed alterations in response to infection. For virions from LLC-MK2 cells, 14 lipids were detected specifically in virions with a further seven lipids being enriched (over mock controls). For virions from C6/36 cells, 43 lipids were detected that were not seen in mock preparations, with a further 16 being specifically enriched (over mock control). These results provide the first lipid description of DENV virions produced in mammalian and mosquito cells, as well as the lipid changes in the corresponding infected cells.

Sphingomyelins in mosquito saliva modify the host lipidome to enhance transmission of flaviviruses by promoting viral protein levels

“…DENV envelop contains lipids, which composition partially varies when viruses derive from mosquito or mammalian cells 57,58 . To evaluate the impact of DENV-lipids on DENV infection, we produced DENV in mosquito and monkey cells, and isolated virions from EVs 59 using discontinuous sucrose density gradient.…”

“…In support of a specific function for mosquito EVlipids, human EV-lipids did not influence DENV infection (Figure 1E), nor cell viability (Figure S4D). DENV envelop contains lipids, which composition partially varies when viruses derive from mosquito or mammalian cells 57,58 . To evaluate the impact of DENV-lipids on DENV infection, we produced DENV in mosquito and monkey cells, and isolated virions from EVs 59 using discontinuous sucrose density gradient.…”

Comparative Virucidal Activities of Essential Oils and Alcohol-Based Solutions against Enveloped Virus Surrogates: In Vitro and In Silico Analyses

“…To evaluate the virucidal disinfectant activity, we used two enveloped viruses, which differ in the lipid content [ 30 , 31 ] and protein structure [ 24 , 26 ] that comprise the viral envelope. The results indicated that DENV-2 was more sensitive to the action of test solutions than CHIKV.…”

AntiviralWolbachiastrains associate withAedes aegyptiendoplasmic reticulum membranes and disturb host cell lipid distribution to restrict dengue virus replication

“…LDs are thought to have conflicting roles in viral infection: many reports have demonstrated that enveloped viruses usurp LDs and alter lipidomic profiles of host cells to enhance their viral life cycles, with lipids accounting for 20-30% of the weight of the virion 62,67,[79][80][81][82][83] . DENV infection, for example, increases LD formation 62 , recruiting FAS to the virus replication site 65 , and its capsid protein accumulates on the surface of LDs, facilitating viral replication by providing a platform for nucleocapsid formation during encapsidation 67 .…”