Cell viability of aortic allografts after long-term cryopreservation and clinical application to aortic root replacement in a patient with aortitis

Motomura, Noboru; Eishi, Kiyoyuki; Kitoh, Hiroshi; Kawazoe, Kohei; Kitoh, Y; Kawashima, Yasunaru; Oka, Takahiro

doi:10.1016/0967-2109(95)90902-h

Cited by 10 publications

(3 citation statements)

References 7 publications

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“…The existence of viable smooth muscle cells and endothelial cells in cryopreserved aortic allografts after thawing has been verified by cell culture [22, 27], but no quantitative analyses have ever been reported. In cryopreserved human valves, Armiger reported that most cells of cryopreserved valves were nonviable when thawed [3], although Niwaya et al reported that 85.9%±6.3% of fibroblastic cells were viable after the valves were thawed [25].…”

Section: Introductionmentioning

confidence: 99%

“…Therefore, the rate of viable smooth muscle cells in cryopreserved aortic allografts after thawing has yet to be satisfactorily elucidated. On the other hand, Motomura et al reported that the number of smooth muscle cells in the medial muscle layer of cryopreserved aortic allografts on the 10th day after transplantation was maintained at approximately the same level as that of native aortas [22]. …”

Section: Introductionmentioning

confidence: 99%

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Acute Regeneration and Chronic Acellular Transformation of Rabbit Cryopreserved Aortic Allografts

Yamada

Takahashi

Toyama

et al. 2012

Acta Histochem. Cytochem

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An analysis of rabbit cryopreserved aortic allografts excised on postoperative days (POD) 2, 5, 11, 60, 210, 360, and 720, as well as controls that were untransplanted native aortas and cryopreserved aortas, was performed. On POD2, the number of medial smooth muscle cells in the allografts was reduced to approximately 50%. Ki-67 analysis revealed that medial smooth muscle cells in the allografts proliferated from the 2nd day. By the 11th day, their proliferation ceased and the number of medial smooth muscle cells was restored to almost at the same level as in the controls. Polymorphic microsatellite DNA marker analysis disclosed that the restored medial smooth muscle cells were of donor origin. From 7 months through 2 years, the media of cryopreserved aortic allografts were transformed into acellular structures, in which the elastic fibers were preserved. On the other hand, newly accumulated smooth muscle cells were observed in the adventitia just outside of acellular media after 7 months. In some cases, scattered lamellar calcium deposition was observed in the same regions. This study presents a comprehensive documentation of regeneration and acellular transformation in cryopreserved aortic allografts based on short and long-term analysis.

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Acute Regeneration and Chronic Acellular Transformation of Rabbit Cryopreserved Aortic Allografts

Yamada

Takahashi

Toyama

et al. 2012

Acta Histochem. Cytochem

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“…Since cardiac surgeons recognized the reliability of the cryopreserved homograft valve, the share of the cryopreserved homograft valve used in the area of cardiac surgery has expanded (6). The excellent result of the cryopreserved aortic homograft valve encouraged Japanese surgeons to produce a domestic version and supply of the homograft valve (7–9). We began our aortic valve replacement series with the cryopreserved homograft valve in 1998.…”

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confidence: 99%

Short‐Term Result of Aortic Valve Replacement with Cryopreserved Homograft Valve in the University of Tokyo Tissue Bank

et al. 2002

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The short-term results after aortic root replacement with 11 cryopreserved aortic homografts was examined. Since 1998, the University of Tokyo Tissue Bank has supplied 11 aortic homograft valves. Nine of the recipients were male, and the average age was 51.2 years. Nine out of 11 patients had suffered from a serious condition of native or prosthetic valve infectious endocarditis. All of the patients underwent aortic root replacement, and the blood type between the patient and the homograft was matched in 8 of the patients. Only 1 patient died (9.1%) in the short-term due to sepsis. The preoperative degree of aortic valve regurgitation in all of the cases was third or fourth while the regurgitation disappeared after the operation in all of them. Thinking of the serious condition of our cases preoperatively, the 9.1% operative mortality was quite acceptable. Long-term follow-up is necessary to estimate the quality of the homografts.

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Endothelial and Smooth Muscle Changes of the Thoracic and Abdominal Aorta with Various Types of Cryopreservation

Arnaud¹

2000

Journal of Surgical Research

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Cell viability of aortic allografts after long-term cryopreservation and clinical application to aortic root replacement in a patient with aortitis

Cited by 10 publications

References 7 publications

Acute Regeneration and Chronic Acellular Transformation of Rabbit Cryopreserved Aortic Allografts

Acute Regeneration and Chronic Acellular Transformation of Rabbit Cryopreserved Aortic Allografts

Short‐Term Result of Aortic Valve Replacement with Cryopreserved Homograft Valve in the University of Tokyo Tissue Bank

Endothelial and Smooth Muscle Changes of the Thoracic and Abdominal Aorta with Various Types of Cryopreservation

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