Cell viability study of thermo-responsive core–shell superparamagnetic nanoparticles for multimodal cancer therapy

Abstract

This is a vital extension of our previously published work. Thermo-responsive copolymer coated superparamagnetic MnFe 2 O 4 nanoparticles are tested for cell viability and affinity on HeLa carcinoma cells under different conditions. Nanoparticles were loaded with anticancer drug doxorubicin. Composite nanoparticles of average diameter 45 nm were of core-shell structure having magnetic core of about 18 nm. Magnetic hyperthermia effects on cell viability and drug delivery were studied by exposing the cell suspension to high frequency magnetic field, and living cells were quantified using MTT method. There was almost absence of drug release at 37°C. Drug was released at temperatures above lower critical solution temperature (LCST) by magnetic heating. LCST of the thermo-responsive copolymer was observed to be around 39°C. Below this temperature, copolymer was hydrophilic and swelled. But above LCST, copolymer could become hydrophobic, expel water and drug and shrink in volume. Combination of hyperthermia and drug delivery effectively treated cancer cells.

Thermal Polymerization on the Surface of Iron Oxide Nanoparticles Mediated by Magnetic Hyperthermia: Implications for Multishell Grafting and Environmental Applications

“…Magnetic nanoparticles (MNP) have been the subject of extensive research during the last decade because of their potential applications in many fields such as diagnostic, 1,2 nucleic acid separation, 3,4 drug delivery [5][6][7][8][9] or even for environmental pollutants removal. 10,11 They have attracted considerable attention for hyperthermia applications, owing to their ability to generate heat when exposed to an alternating magnetic field (AMF).…”

Nanosystems Based on Magnetic Nanoparticles and Thermo- or pH-Responsive Polymers: An Update and Future Perspectives

“…Changing the already FDA-approved IONP composition (Fe x O y, see Table S11) to that of mixed ferrites (such as ) could also improve MHT heat performances. 24,49 As a future material, in order to avoid overheating, Cr 3+ substituted Co−Zn ferrites might enable selfregulating MHT if they are employed in TR-nanosystems. 50 Other groups have exploited MnFe 2 O 4 and La 0.73 Sr 0.27 MnO 3 instead of IONPs when synthesizing PNIPAm-based TRnanosystems.…”

Polymer-Assisted Magnetic Nanoparticle Assemblies for Biomedical Applications

“…124 In the above-mentioned nanoassemblies, the polymers not only act as glue, matrix, or template for the assembling with NPs, but also provide fascinating opportunities and possibilities for the accommodation of drugs or other functional moieties. Recently, a variety of functional polymers have emerged and been used to construct "smart" MNP assemblies with responsiveness to specific stimuli, such as pH, 125 redox gradient, 126 temperature, 127 glutathione, 128 and some enzymes, 129 enabling promising biomedical applications such as precise diseases diagnosis, controlled and targeted drug delivery, as well as therapeutics. 130,131 4.…”