Cell wall composition and penetration resistance against the fungal pathogen<i>Colletotrichum higginsianum</i>are affected by impaired starch turnover in Arabidopsis mutants

Engelsdorf, Timo; Will, Cornelia; Hofmann, Jörg; Schmitt, Christine; Merritt, Brian B.; Rieger, Leonie; Frenger, Marc; Marschall, André; Franke, Rochus; Pattathil, Sivakumar; Voll, Lars M.

doi:10.1093/jxb/erw434

Cited by 36 publications

(51 citation statements)

References 90 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Twenty‐four hours after inoculation, disease lesions measured on average 45.5 mm 2 in the Col‐0 wild‐type, 25.6 mm 2 in the resistant accession Rubezhnoe (Welch t ‐test P value = 0.0015) and 72.5 mm 2 in the susceptible accession Shahdara ( P value = 0.0021). Although mutants in SOP1 are more susceptible to the fungal pathogen Colletotrichum higginsianum (Engelsdorf et al , ), sop1‐2 behaved similar to wild‐type upon inoculation by S. sclerotiorum in our assays ( P value = 0.26). However, snak2 and serk2 mutant plants showed a ~30% increase in susceptibility to S. sclerotiorum compared to wild‐type ( P value = 8.0e −06 and 0.0089, respectively).…”

Section: Resultsmentioning

confidence: 62%

“…Shahdara (P value = 0.0021). Although mutants in SOP1 are more susceptible to the fungal pathogen Colletotrichum higginsianum (Engelsdorf et al, 2016), sop1-2 behaved similar to wild-type upon inoculation by S. sclerotiorum in our assays (P value = 0.26). However, snak2 and serk2 mutant plants showed a ~30% increase in susceptibility to S. sclerotiorum compared to wild-type (P value = 8.0e −06 and 0.0089, respectively).…”

Section: Sclerotinia Sclerotiorum Srnas Map To Transposable Element Smentioning

confidence: 70%

See 1 more Smart Citation

Small RNAs from the plant pathogenic fungus Sclerotinia sclerotiorum highlight host candidate genes associated with quantitative disease resistance

Derbyshire

Mbengue

Barascud

et al. 2019

Molecular Plant Pathology

View full text Add to dashboard Cite

Summary Fungal plant pathogens secrete effector proteins and metabolites to cause disease. Additionally, some species transfer small RNAs (sRNAs) into plant cells to silence host mRNAs through complementary base pairing and suppress plant immunity. The fungus Sclerotinia sclerotiorum infects over 600 plant species, but little is known about the molecular processes that govern interactions with its many hosts. In particular, evidence for the production of sRNAs by S. sclerotiorum during infection is lacking. We sequenced sRNAs produced by S. sclerotiorum in vitro and during infection of two host species, Arabidopsis thaliana and Phaseolus vulgaris . We found that S. sclerotiorum produces at least 374 distinct highly abundant sRNAs during infection, mostly originating from repeat‐rich plastic genomic regions. We predicted the targets of these sRNAs in A. thaliana and found that these genes were significantly more down‐regulated during infection than the rest of the genome. Predicted targets of S. sclerotiorum sRNAs in A. thaliana were enriched for functional domains associated with plant immunity and were more strongly associated with quantitative disease resistance in a genome‐wide association study (GWAS) than the rest of the genome. Mutants in A. thaliana predicted sRNA target genes SERK2 and SNAK2 were more susceptible to S. sclerotiorum than wild‐type, suggesting that S. sclerotiorum sRNAs may contribute to the silencing of immune components in plants. The prediction of fungal sRNA targets in plant genomes can be combined with other global approaches, such as GWAS, to assist in the identification of plant genes involved in quantitative disease resistance.

show abstract

Section: Resultsmentioning

confidence: 62%

Section: Sclerotinia Sclerotiorum Srnas Map To Transposable Element Smentioning

confidence: 70%

Small RNAs from the plant pathogenic fungus Sclerotinia sclerotiorum highlight host candidate genes associated with quantitative disease resistance

Derbyshire

Mbengue

Barascud

et al. 2019

Molecular Plant Pathology

View full text Add to dashboard Cite

show abstract

“…In addition to RWA proteins, members of the TRICHROME BIREFRINGENCE (TBR) and TBR‐LIKE (TBL) protein families are involved in the O ‐acetylation of wall polysaccharides (Gille and Pauly, ). The Arabidopsis powdery mildew resistant5 ( pmr5 ) mutant, impaired in a TBL member, is more resistant than wild‐type plants to powdery mildew fungi and C. higginsianum , whereas its resistance to P. syringae is similar to that of wild‐type plants (Table ; Vogel et al ., ; Gille and Pauly, ; Engelsdorf et al ., ;. Another recent example of the activation of specific cell wall‐triggered immune responses comes from the alteration of cell wall xylan acetylation caused by another TBL member, Eskimo1 ( ESK1 ), which encodes a plant‐specific polysaccharide O ‐acetyltransferase involved in xylan acetylation (Xu et al ., ; Escudero et al ., ).…”

Section: Alterations To Plant Cell Wall Integrity Affect Disease Resimentioning

confidence: 99%

Plant cell wall‐mediated immunity: cell wall changes trigger disease resistance responses

et al. 2018

View full text Add to dashboard Cite

Plants have evolved a repertoire of monitoring systems to sense plant morphogenesis and to face environmental changes and threats caused by different attackers. These systems integrate different signals into overreaching triggering pathways which coordinate developmental and defence-associated responses. The plant cell wall, a dynamic and complex structure surrounding every plant cell, has emerged recently as an essential component of plant monitoring systems, thus expanding its function as a passive defensive barrier. Plants have a dedicated mechanism for maintaining cell wall integrity (CWI) which comprises a diverse set of plasma membrane-resident sensors and pattern recognition receptors (PRRs). The PRRs perceive plant-derived ligands, such as peptides or wall glycans, known as damage-associated molecular patterns (DAMPs). These DAMPs function as 'danger' alert signals activating DAMP-triggered immunity (DTI), which shares signalling components and responses with the immune pathways triggered by non-self microbe-associated molecular patterns that mediate disease resistance. Alteration of CWI by impairment of the expression or activity of proteins involved in cell wall biosynthesis and/or remodelling, as occurs in some plant cell wall mutants, or by wall damage due to colonization by pathogens/pests, activates specific defensive and growth responses. Our current understanding of how these alterations of CWI are perceived by the wall monitoring systems is scarce and few plant sensors/PRRs and DAMPs have been characterized. The identification of these CWI sensors and PRR-DAMP pairs will help us to understand the immune functions of the wall monitoring system, and might allow the breeding of crop varieties and the design of agricultural strategies that would enhance crop disease resistance.

show abstract

“…For example, PMR6 encodes a GPI‐anchored pectate lyase protein involved in pectin degradation and potentially release of pectic oligosaccharides. Increased pectin content in epidermal cell walls of pmr6 mutants has been shown to increase resistance against powdery mildew and Colletotrichum higginsianum (Vogel et al ; Engelsdorf et al ). It is proposed that this may result in higher mechanical resistance during infection or alter signaling.…”

Section: Gaps and Cell Wall Biosynthesis And Maintenancementioning

confidence: 99%

Plant glycosylphosphatidylinositol anchored proteins at the plasma membrane‐cell wall nexus

Yeats

Bacic

Johnson

2018

JIPB

View full text Add to dashboard Cite

Approximately 1% of plant proteins are predicted to be post-translationally modified with a glycosylphosphatidylinositol (GPI) anchor that tethers the polypeptide to the outer leaflet of the plasma membrane. Whereas the synthesis and structure of GPI anchors is largely conserved across eukaryotes, the repertoire of functional domains present in the GPI-anchored proteome has diverged substantially. In plants, this includes a large fraction of the GPI-anchored proteome being further modified with plant-specific arabinogalactan (AG) O-glycans. The importance of the GPI-anchored proteome to plant development is underscored by the fact that GPI biosynthetic null mutants exhibit embryo lethality. Mutations in genes encoding specific GPI-anchored proteins (GAPs) further supports their contribution to diverse biological processes, occurring at the interface of the plasma membrane and cell wall, including signaling, cell wall metabolism, cell wall polymer cross-linking, and plasmodesmatal transport. Here, we review the literature concerning plant GPI-anchored proteins, in the context of their potential to act as molecular hubs that mediate interactions between the plasma membrane and the cell wall, and their potential to transduce the signal into the protoplast and, thereby, activate signal transduction pathways.

show abstract

Cell wall composition and penetration resistance against the fungal pathogenColletotrichum higginsianumare affected by impaired starch turnover in Arabidopsis mutants

Cited by 36 publications

References 90 publications

Small RNAs from the plant pathogenic fungus Sclerotinia sclerotiorum highlight host candidate genes associated with quantitative disease resistance

Small RNAs from the plant pathogenic fungus Sclerotinia sclerotiorum highlight host candidate genes associated with quantitative disease resistance

Plant cell wall‐mediated immunity: cell wall changes trigger disease resistance responses

Plant glycosylphosphatidylinositol anchored proteins at the plasma membrane‐cell wall nexus

Contact Info

Product

Resources

About