Cell Wall Membrane Fraction of <i>Chlorella sorokiniana</i> Enhances Host Antitumor Immunity and Inhibits Colon Carcinoma Growth in Mice

Ishiguro, Susumu; Robben, Nicole; Burghart, Riley; Cote, Paige; Greenway, Sarah; Thakkar, Ravindra; Upreti, Deepa; Nakashima, Ayaka; Suzuki, Kengo; Comer, Jeffrey; Tamura, Masaaki

doi:10.1177/1534735419900555

Cited by 13 publications

(11 citation statements)

References 32 publications

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“…and anticancer activities have been proven in the mouse colon carcinoma cell line CT26.CL25, and the following human colon carcinoma cell lines: SW620, HT29, COLO 205 and Caco-2 [6]. Additionally, stimulation of cytokines release and nitrogen oxide production in macrophages cell line RAW264.7, in response to polysaccharides of green alga Chlorella ellipsoidea, have been reported [7,8].…”

Section: Introductionmentioning

confidence: 96%

Impact of phytochemicals and plant extracts on viability and proliferation of NK cell line NK-92 – a closer look at immunomodulatory properties of goji berries extract in human colon cancer cells

Kwaśnik¹,

Lemieszek²,

Rzeski³

2021

Ann Agric Environ Med.

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Section: Introductionmentioning

confidence: 96%

Impact of phytochemicals and plant extracts on viability and proliferation of NK cell line NK-92 – a closer look at immunomodulatory properties of goji berries extract in human colon cancer cells

Kwaśnik¹,

Lemieszek²,

Rzeski³

2021

Ann Agric Environ Med.

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“…Lutein, a compound with proven anti-cataract properties, is also found in Chlorella sp. , while its extracts exhibit anti-tumor, anti-inflammatory, antioxidant, and anti-microbial properties, and reduce blood pressure and cholesterol levels [ 32 , 33 , 34 ]. As a result, Chlorella sp.…”

Section: Introductionmentioning

confidence: 99%

Optimization of Microalga Chlorella vulgaris Magnetic Harvesting

et al. 2021

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Harvesting of microalgae is a crucial step in microalgae-based mass production of different high value-added products. In the present work, magnetic harvesting of Chlorella vulgaris was investigated using microwave-synthesized naked magnetite (Fe3O4) particles with an average crystallite diameter of 20 nm. Optimization of the most important parameters of the magnetic harvesting process, namely pH, mass ratio (mr) of magnetite particles to biomass (g/g), and agitation speed (rpm) of the C. vulgaris biomass–Fe3O4 particles mixture, was performed using the response surface methodology (RSM) statistical tool. Harvesting efficiencies higher than 99% were obtained for pH 3.0 and mixing speed greater or equal to 350 rpm. Recovery of magnetic particles via detachment was shown to be feasible and the recovery particles could be reused at least five times with high harvesting efficiency. Consequently, the described harvesting approach of C. vulgaris cells leads to an efficient, simple, and quick process, that does not impair the quality of the harvested biomass.

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“…Moreover, the immunostimulatory effects of β-1,3 have been widely studied for cancer treatment [ 1 , 15 , 16 ]. Anticancer activities of extracts of some single-celled algae may also be linked to β-1,3 glucans [ 17 ].…”

Section: Introductionmentioning

confidence: 99%

Beta-1,3 Oligoglucans Specifically Bind to Immune Receptor CD28 and May Enhance T Cell Activation

Comer

Bassette

Burghart

et al. 2021

IJMS

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Beta glucans are known to have immunomodulatory effects that mediated by a variety of mechanisms. In this article, we describe experiments and simulations suggesting that beta-1,3 glucans may promote activation of T cells by a previously unknown mechanism. First, we find that treatment of a T lymphoblast cell line with beta-1,3 oligoglucan significantly increases mRNA levels of T cell activation-associated cytokines, especially in the presence of the agonistic anti-CD3 antibody. This immunostimulatory activity was observed in the absence of dectin-1, a known receptor for beta-1,3 glucans. To clarify the molecular mechanism underlying this activity, we performed a series of molecular dynamics simulations and free-energy calculations to explore the interaction of beta-1,3 oligoglucans with potential immune receptors. While the simulations reveal little association between beta-1,3 oligoglucan and the immune receptor CD3, we find that beta-1,3 oligoglucans bind to CD28 near the region identified as the binding site for its natural ligands CD80 and CD86. Using a rigorous absolute binding free-energy technique, we calculate a dissociation constant in the low millimolar range for binding of 8-mer beta-1,3 oligoglucan to this site on CD28. The simulations show this binding to be specific, as no such association is computed for alpha-1,4 oligoglucan. This study suggests that beta-1,3 glucans bind to CD28 and may stimulate T cell activation collaboratively with T cell receptor activation, thereby stimulating immune function.

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Cell Wall Membrane Fraction of Chlorella sorokiniana Enhances Host Antitumor Immunity and Inhibits Colon Carcinoma Growth in Mice

Cited by 13 publications

References 32 publications

Impact of phytochemicals and plant extracts on viability and proliferation of NK cell line NK-92 – a closer look at immunomodulatory properties of goji berries extract in human colon cancer cells

Impact of phytochemicals and plant extracts on viability and proliferation of NK cell line NK-92 – a closer look at immunomodulatory properties of goji berries extract in human colon cancer cells

Optimization of Microalga Chlorella vulgaris Magnetic Harvesting

Beta-1,3 Oligoglucans Specifically Bind to Immune Receptor CD28 and May Enhance T Cell Activation

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