Cell wall remodeling in Arabidopsis stamen abscission zones

Lashbrook, Coralie C.; Cai, Suqin

doi:10.4161/psb.3.9.6489

Cited by 56 publications

(40 citation statements)

References 26 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Cell walls also provide structural integrity, mechanical support and a physical barrier against pathogens (Somerville et al, 2004). Plant cells must remodel this rigid structure during cell growth and development, and disassemble cell walls during senescence processes such as organ abscission and fruit softening (Brummell and Harpster, 2001;Cantu et al, 2008;Lashbrook and Cai, 2008). In order to perform appropriate growth responses, cell wall remodeling is monitored and interpreted by the cell integrity signaling pathway (Hematy et al, 2007).…”

Section: Discussionmentioning

confidence: 99%

Mutation of the chitinase-like protein-encoding AtCTL2 gene enhances lignin accumulation in dark-grown Arabidopsis seedlings

Hossain

Noh

Kim

et al. 2010

Journal of Plant Physiology

View full text Add to dashboard Cite

Section: Discussionmentioning

confidence: 99%

Mutation of the chitinase-like protein-encoding AtCTL2 gene enhances lignin accumulation in dark-grown Arabidopsis seedlings

Hossain

Noh

Kim

et al. 2010

Journal of Plant Physiology

View full text Add to dashboard Cite

“…According to these two criteria, 11 of the selected differentially regulated shading-responsive genes are regarded as being involved in the process of fruitlet abscission. Six of these genes are also documented as being related to the shedding of plant organs,including CHI [26,27,61], FFU [62], GH3 [63], SAMDC [64], MYB [28] and PMEI [65]. The remaining five genes ( AS , ACL , TNL1 , UBCE2 and LL-DAP-AT ) have not yet been reported in the literature to be involved in organ shedding.…”

Section: Discussionmentioning

confidence: 99%

De novo assembly and characterization of fruit transcriptome in Litchi chinensis Sonn and analysis of differentially regulated genes in fruit in response to shading

Wang

Huang

et al. 2013

BMC Genomics

View full text Add to dashboard Cite

BackgroundLitchi (Litchi chinensis Sonn.) is one of the most important fruit trees cultivated in tropical and subtropical areas. However, a lack of transcriptomic and genomic information hinders our understanding of the molecular mechanisms underlying fruit set and fruit development in litchi. Shading during early fruit development decreases fruit growth and induces fruit abscission. Here, high-throughput RNA sequencing (RNA-Seq) was employed for the de novo assembly and characterization of the fruit transcriptome in litchi, and differentially regulated genes, which are responsive to shading, were also investigated using digital transcript abundance(DTA)profiling.ResultsMore than 53 million paired-end reads were generated and assembled into 57,050 unigenes with an average length of 601 bp. These unigenes were annotated by querying against various public databases, with 34,029 unigenes found to be homologous to genes in the NCBI GenBank database and 22,945 unigenes annotated based on known proteins in the Swiss-Prot database. In further orthologous analyses, 5,885 unigenes were assigned with one or more Gene Ontology terms, 10,234 hits were aligned to the 24 Clusters of Orthologous Groups classifications and 15,330 unigenes were classified into 266 Kyoto Encyclopedia of Genes and Genomes pathways. Based on the newly assembled transcriptome, the DTA profiling approach was applied to investigate the differentially expressed genes related to shading stress. A total of 3.6 million and 3.5 million high-quality tags were generated from shaded and non-shaded libraries, respectively. As many as 1,039 unigenes were shown to be significantly differentially regulated. Eleven of the 14 differentially regulated unigenes, which were randomly selected for more detailed expression comparison during the course of shading treatment, were identified as being likely to be involved in the process of fruitlet abscission in litchi.ConclusionsThe assembled transcriptome of litchi fruit provides a global description of expressed genes in litchi fruit development, and could serve as an ideal repository for future functional characterization of specific genes. The DTA analysis revealed that more than 1000 differentially regulated unigenes respond to the shading signal, some of which might be involved in the fruitlet abscission process in litchi, shedding new light on the molecular mechanisms underlying organ abscission.

show abstract

“…β-1,3-Endoglucanases are a class of hydrolytic enzyme that catalyse the cleavage of 1,3-β- D -glucosidic linkages in β-1,3-glucans (Lashbrook and Cai, 2008). They have received a considerable amount of attention due to their role in plant pathogen defence (van Loon et al , 2006).…”

Section: Discussionmentioning

confidence: 99%

The interaction of the novel 30C02 cyst nematode effector protein with a plant β-1,3-endoglucanase may suppress host defence to promote parasitism

Hamamouch

Hewezi

et al. 2012

Journal of Experimental Botany

View full text Add to dashboard Cite

Phytoparasitic nematodes secrete an array of effector proteins to modify selected recipient plant cells into elaborate and essential feeding sites. The biological function of the novel 30C02 effector protein of the soybean cyst nematode, Heterodera glycines, was studied using Arabidopsis thaliana as host and the beet cyst nematode, Heterodera schachtii, which contains a homologue of the 30C02 gene. Expression of Hg30C02 in Arabidopsis did not affect plant growth and development but increased plant susceptibility to infection by H. schachtii. The 30C02 protein interacted with a specific (AT4G16260) host plant β-1,3-endoglucanase in both yeast and plant cells, possibly to interfere with its role as a plant pathogenesis-related protein. Interestingly, the peak expression of 30C02 in the nematode and peak expression of At4g16260 in plant roots coincided at around 3–5 d after root infection by the nematode, after which the relative expression of At4g16260 declined significantly. An Arabidopsis At4g16260 T-DNA mutant showed increased susceptibility to cyst nematode infection, and plants that overexpressed At4g16260 were reduced in nematode susceptibility, suggesting a potential role of host β-1,3-endoglucanase in the defence response against H. schachtii infection. Arabidopsis plants that expressed dsRNA and its processed small interfering RNA complementary to the Hg30C02 sequence were not phenotypically different from non-transformed plants, but they exhibited a strong RNA interference-mediated resistance to infection by H. schachtii. The collective results suggest that, as with other pathogens, active suppression of host defence is a critical component for successful parasitism by nematodes and a vulnerable target to disrupt the parasitic cycle.

show abstract

Cell wall remodeling in Arabidopsis stamen abscission zones

Cited by 56 publications

References 26 publications

Mutation of the chitinase-like protein-encoding AtCTL2 gene enhances lignin accumulation in dark-grown Arabidopsis seedlings

Mutation of the chitinase-like protein-encoding AtCTL2 gene enhances lignin accumulation in dark-grown Arabidopsis seedlings

De novo assembly and characterization of fruit transcriptome in Litchi chinensis Sonn and analysis of differentially regulated genes in fruit in response to shading

The interaction of the novel 30C02 cyst nematode effector protein with a plant β-1,3-endoglucanase may suppress host defence to promote parasitism

Contact Info

Product

Resources

About