Cell wall remodeling under salt stress: Insights into changes in polysaccharides, feruloylation, lignification, and phenolic metabolism in maize

Oliveira, Dyoni Matias de; Mota, Thatiane Rodrigues; Salatta, Fábio V.; Sinzker, Renata Costa; Končitíková, Radka; Kopečný, David; Simister, Rachael; Silva, Mariana; Goeminne, Geert; Morreel, Kris; Rencoret, Jorge; Gutiérrez, Ana; Tryfona, Theodora; Marchiosi, Rogério; Dupree, Paul; Rı́o, José C. del; Boerjan, Wout; McQueen‐Mason, Simon J.; Gómez, Leonardo D.; Ferrarese‐Filho, Osvaldo; Santos, Wanderley Dantas dos

doi:10.22541/au.158497990.05253688

Cited by 2 publications

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“…For instance, a study conducted in rice mutants presenting decreased levels of xylan demonstrated a lower degree of diferulate cross‐links, also exhibiting an increase in terminal arabinose (Chiniquy et al ., 2012a). Moreover, decreased levels of lignin monomers led to compensatory increased levels of cellulose and hemicellulose in different plant species (Hu et al ., 1999; Vermerris et al ., 2010; Oliveira et al ., 2020b). These observations suggest a compensatory mechanism in plants that indicates a level of interdependence between different components of the cell wall.…”

Section: Discussionmentioning

confidence: 99%

Suppression of a BAHD acyltransferase decreases p‐coumaroyl on arabinoxylan and improves biomass digestibility in the model grass Setaria viridis

et al. 2020

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SummaryGrass cell walls have hydroxycinnamic acids attached to arabinosyl residues of arabinoxylan (AX), and certain BAHD acyltransferases are involved in their addition. In this study, we characterized one of these BAHD genes in the cell wall of the model grass Setaria viridis. RNAi silenced lines of S. viridis (SvBAHD05) presented a decrease of up to 42% of ester‐linked p‐coumarate (pCA) and 50% of pCA‐arabinofuranosyl, across three generations. Biomass from SvBAHD05 silenced plants exhibited up to 32% increase in biomass saccharification after acid pre‐treatment, with no change in total lignin. Molecular dynamics simulations suggested that SvBAHD05 is a p‐coumaroyl coenzyme A transferase (PAT) mainly involved in the addition of pCA to the arabinofuranosyl residues of AX in Setaria. Thus, our results provide evidence of p‐coumaroylation of AX promoted by SvBAHD05 acyltransferase in the cell wall of the model grass S. viridis. Furthermore, SvBAHD05 is a promising biotechnological target to engineer crops for improved biomass digestibility for biofuels, biorefineries and animal feeding.

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Section: Discussionmentioning

confidence: 99%

Suppression of a BAHD acyltransferase decreases p‐coumaroyl on arabinoxylan and improves biomass digestibility in the model grass Setaria viridis

et al. 2020

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“…The components and mechanical features of the cell wall play a key role in controlling the size, shape and cohesion of plant cells (Minic & Jouanin, 2006; Mollet et al 2013; Bacete et al 2020; Oliveira et al 2020). The degradation of cell wall polysaccharides and proteins might increase relaxation of the TTS cell walls or weaken adhesion between TTS cells, leading to the separation of TTS cells and enlargement of the ECM (Jiang et al, 2005; ZúÑiga‐Sánchez & Buen, 2012; Salazar‐Iribe et al, 2016; Amos & Mohnen, 2019; Anderson & Kieber 2020).…”

Section: Discussionmentioning

confidence: 99%

The morphological and physiological basis of delayed pollination overcoming pre‐fertilization cross‐incompatibility in Nicotiana

et al. 2020

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Delayed pollination is widely used to overcome pre‐fertilization incompatibility, but its regulatory mechanisms are unclear. When Nicotiana tabacum was cross‐pollinated with pollen of N. alata, the incompatibility occurring in the basal 1/4 region of the style (pollinated at anthesis: 0‐day‐old pistil) was overcome by delayed pollination (of 6‐day‐old pistil), and the morphological changes and corresponding physiological basis are explored here. The structure and ultrastructure of the pistil were observed under fluorescence microscopy and transmission electron microscopy. Differentially expressed proteins were screened with a monoclonal antibody chip for Nicotiana, and protein expression and distribution were analysed by immunofluorescence. Cellulase and pectinase activities were tested using enzyme‐linked immunosorbent assay kits. The style of Nicotiana is solid in the basal region and pollen tubes grow in the extracellular spaces (ECM) of the transmitting tissue (TTS) cells. Seven of the 22 identified proteins were cell wall‐associated proteins and were expressed at higher levels during pistil senescence. Cellulase and pectinase activities increased. The TTS cells in the basal 1/4 region of the 0‐day‐old style were polygonal and tightly arranged, with narrow ECM, but these were oval or partially dissolved in the 6‐day‐old pistil, leading to wider ECM and richer secretions. The increased expression of cell wall proteins and enhanced enzyme activity during pistil senescence might partially be responsible for the cells becoming oval and the ECM enlarged, providing the morphological basis for delayed pollination overcoming the pre‐fertilization incompatibility between N. tabacum and N. alata.

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Cell wall remodeling under salt stress: Insights into changes in polysaccharides, feruloylation, lignification, and phenolic metabolism in maize

Cited by 2 publications

References 0 publications

Suppression of a BAHD acyltransferase decreases p‐coumaroyl on arabinoxylan and improves biomass digestibility in the model grass Setaria viridis

Suppression of a BAHD acyltransferase decreases p‐coumaroyl on arabinoxylan and improves biomass digestibility in the model grass Setaria viridis

The morphological and physiological basis of delayed pollination overcoming pre‐fertilization cross‐incompatibility in Nicotiana

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