Cell Wall Ultrastructure of Strains of Haemophilus ducreyi and Haemophilus piscium

Kilian, Mogens; Theilade, J.

doi:10.1099/00207713-25-4-351

Cited by 14 publications

(6 citation statements)

References 15 publications

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“…The present study partly confirms this assumption. None of the Reymann (1947Reymann ( , 1949aReymann ( , 6, 1950Reymann ( , 1951 strains studied here can be assigned to the genus Haemophilus as they have been shown to be Gram-positive (Kilian & Theilade, 1975). However, the two strains from the Collection de 1'Institut Pasteur conform to the present definition of the genus Haemophilus, and the DNA base composition of these strains falls within the limits accepted for Haemophilus strains.…”

Section: K I L I a Nmentioning

confidence: 57%

“…An examination of the fine structure of the cell walls of the strains revealed, however, that they were typical for a Gram-negative organism (Kilian & Theilade, 1975). The strains grew equally well on fish extract agar (Snieszko et a/., 1950) and on chocolate agar at 22 "C. No growth was obtained at 37 "C. On chocolate agar after 4 days incubation colonies were smooth, convex, whitish and translucent, and reached a diameter of I to 2 mm.…”

Section: Species Rejected From Haemophilus: H Pisciummentioning

confidence: 91%

“…They were predominantly Gram-negative but interspersed with varying numbers of cells that resisted total decolorization with ethanol. A closer examination by electron microscopy showed that the fine structure of the cell wall of these strains was typical of a Gram-positive bacterium (Kilian & Theilade, 1975). Colonies on chocolate agar were smooth, convex, greyish and translucent and reached a diameter of about 0.5 mm.…”

Section: Haemophilus Ducreyimentioning

confidence: 94%

“…These two strains (HK379 and ~~3 8 0 ) were non-motile, irregular, slender rods often arranged in long chains. They were distinctly Gram-negative as shown by staining and by electron microscopic examination of the cell wall (Kilian & Theilade, 1975). Colonies on chocolate agar after 72 h incubation were predominantly small (about 0.5 mm), flat, smooth, greyish and translucent but with a few interspersed larger colonies (1.5 to 2.0 mm) with an otherwise identical appearance.…”

Section: Haemophilus Ducreyimentioning

confidence: 96%

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A Taxonomic Study of the Genus Haemophilus, with the Proposal of a New Species

Kilian¹

1976

Journal of General Microbiology

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687

433

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A collection of 426 Haemophilus strains isolated from people with infectious diseases and from the normal flora of mucous membranes in humans and various animal species was studied in an attempt to revise and improve the taxonomy of the genus Haemophilus. The examinations included the determination of a number of biochemical and physiological properties, of which several had not previously been applied to the taxonomy of haemophili. The resulting data revealed many hitherto unrecognized characters of taxonomic significance, and several of the species can now be more accurately defined. The classification presented is supported by the DNA base composition of a large number of representative strains. A diagnostic key to the different taxa is presented. Haemophilus injluenzae and H. paruinfluenzae have been subdivided into a number of biotypes. It is possible to demonstrate a relationship between the individual biotypes of H. influenzae and the origin of the strains assigned to them. The results indicate that H. aegyptius, H. parahaemolyticus and H. paraphrohaemolyticus do not merit specific status. Four unnamed taxa of V-factor-dependent haemophili have been recognized. The name Haemophilus segnis is proposed for one of these taxa, which consists mainly of strains isolated from the human oral cavity. It is demonstrated that the name H. ducreyi has been used for different groups of bacteria, and that only one of these groups can legitimately be assigned to the genus Haemophilus. Haemolytic V-factor-dependent strains from swine, previously included in H. parahaemolyticus, are significantly different from strains of human origin and should be named H.pleuropneumoniae. None of the strains from swine and fowls were haemindependent. The relationships of these strains to the species H. suis and H. gallinarum, and to H. parasuis and H. paragallinarum are discussed. Haemophilus piscium is shown not to belong to the genus Haemophilus. The taxonomic position of H. aphrophilus is uncertain and its possible relationship to Actinobacillus actinomycetemcomitans requires further study. The positive correlation found between the ecology of the strains studied and their affiliation with the different taxa is discussed.

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Section: K I L I a Nmentioning

confidence: 57%

Section: Species Rejected From Haemophilus: H Pisciummentioning

confidence: 91%

Section: Haemophilus Ducreyimentioning

confidence: 94%

Section: Haemophilus Ducreyimentioning

confidence: 96%

See 2 more Smart Citations

A Taxonomic Study of the Genus Haemophilus, with the Proposal of a New Species

Kilian¹

1976

Journal of General Microbiology

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687

433

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“…2) have shown that the outer membrane of H. ducreyi appears morphologically similar to those of other gram-negative bacteria (82). The outer membrane protein profile is also typical of gram-negative bacteria, with one to several proteins predominating (1,115,145,164).…”

Section: Outer Membrane Proteins and Lpsmentioning

confidence: 83%

Chancroid and Haemophilus ducreyi

1989

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Haemophilus ducreyi is the causative agent of chancroid, one of the genital ulcerative diseases. H. ducreyi is the major cause of genital ulcer disease in Africa and Southeast Asia and is of increasing concern in the United States. Definitive diagnosis of chancroid requires the isolation and identification of H. ducreyi, but isolation of this organism is difficult and the available medium is not optimal for all strains. Fluorescent antibody and serologic tests are of limited value. In general, our knowledge of this organism is rather limited, and indeed, recent studies have questioned the placement of H. ducreyi in the genus Haemophilus. H. ducreyi has relatively few biochemical activities, and epidemiologic studies are limited because there are limited phenotypic markers available for strain typing. Specific virulence factors of H. ducreyi have yet to be identified. Antimicrobial resistance in H. ducreyi is of special concern, as this organism has acquired both gram-negative and gram-positive resistance determinants. In addition, some of these determinants can be mobilized and transferred to other Haemophilus species or to Neisseria gonorrhoeae.

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Haemophilus

Kilian

2015

Bergey's Manual of Systematics of Archaea and Bacteria

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Hae.mo' phi.lus . Gr. n. haima blood; Gr. n. philos lover; M.L masc. Haemophilus blood‐lover. Proteobacteria / Gammaproteobacteria / Pasteurellales / Pasteurellaceae / Haemophilus Minute to medium‐sized coccobacilli or rods , generally less than 1 µm in width and variable in length, sometimes forming threads or filaments and showing marked pleomorphism . Gram negative. Nonmotile. Aerobic or facultatively anaerobic. Require preformed growth factors present in blood , particularly X factor (protoporphyrin IX or protoheme) and/or V factor (nicotinamide adenine dinucleotide [NAD] or NAD phosphate [NADP]). Even after specific growth factors have been provided, growth is best on complex media. Optimum temperature, 35–37°C. Nitrates are reduced to or beyond nitrites . Oxidase and catalase reactions vary among strains. Chemoorganotrophic. All species can attack carbohydrates fermentatively , yielding acetic, lactic, and succinic acids as end products in glucose broth. Occur as obligate parasites on the mucous membranes of humans and a variety of animal species . Several 16S rRNA sequence signatures for the family Pasteurellaceae have been demonstrated, but none of these is specific for the genus Haemophilus as presently defined (Dewhirst et al., 1992). The mol % G + C of the DNA is : 37–44. Type species : Haemophilus influenzae (Lehmann and Neumann 1896) Winslow, Broadhurst, Buchanan, Krumwiede, Rogers, and Smith 1917, 561 ( Bacterium influenzae Lehmann and Neumann 1896, 187.)

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Cell Wall Ultrastructure of Strains of Haemophilus ducreyi and Haemophilus piscium

Cited by 14 publications

References 15 publications

A Taxonomic Study of the Genus Haemophilus, with the Proposal of a New Species

A Taxonomic Study of the Genus Haemophilus, with the Proposal of a New Species

Chancroid and Haemophilus ducreyi

Haemophilus

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