Cells, cytokines and other molecules associated with rejection of gastrointestinal nematode parasites

Meeusen, Elza Nicole Theresia; Balic, Adam; Bowles, Vernon M

doi:10.1016/j.vetimm.2005.07.002

Cited by 127 publications

(110 citation statements)

References 15 publications

Supporting

Mentioning

101

Contrasting

Unclassified

Order By: Relevance

“…Many studies suggested that this cell population play a role in resistance to helminth infection since significant correlations between resistance/susceptibility to endoparasites infection and the magnitude of the peripheral eosinophil response has been shown (Meeusen et al, 2005). More recently it has been shown that eosinophils could interact with and damage gastrointestinal nematode larvae in vivo (Balic et al, 2006).…”

Section: Discussionmentioning

confidence: 99%

See 1 more Smart Citation

Serum antibody responses in Creole kids experimentally infected with Haemonchus contortus

Bambou¹,

Chevrotière²,

Varo³

et al. 2008

Veterinary Parasitology

View full text Add to dashboard Cite

The objective of this study was to evaluate the relationship of parasite-specific serum antibodies with the resistance status of Creole kids. The average breeding values on egg output predicted in a context of natural infection at 11 months of age were distant of 1.07 genetic standard deviation between resistant and susceptible animals. After drenching the animals were maintained worm-free during 1 month until experimental infection with 10,000 Haemonchus contortus infective larvae (L3). Enzyme-linked immunosorbent assay was carried out in serum samples to determine the level of IgG, IgA and IgE anti-H. contortus L3 crude extracts and adult excretion/secretion products (ESP). Parasitological and blood immunological parameters were measured on the 2 extreme groups. Despite the absence of any typical signs of haemonchosis, susceptible kids had more than 11 times higher faecal egg counts (FEC) at 35 days post-infection (d.p.i.) than resistant kids had. Levels of immunoglobulin against H. contortus L3 and ESP increased significantly after infection in both groups. However, no difference in the host immune response mediated by immunoglobulin against H. contortus was evidenced between groups. This finding suggests that, in goats previously infected by H. contortus, a degree of protection occurred and the phenotypic and genetic segregation in resistant and susceptible animals were not related to the humoral immune response. The correlation coefficients between FEC and IgE anti-ESP (r = 0.593; P < 0.05 was significant in both resistant and susceptible animals. Such correlation suggesting a hypersensitivity reaction dependent on worm prolificacy has never been described. This result needs further studies to understand the mechanisms underlying this observation. #

show abstract

Section: Discussionmentioning

confidence: 99%

“…Thus genetic resistance is immunogically mediated by proliferation of mucosal mast cells, globule leukocytes and eosinophils (Meeusen et al, 2005). The response against gastrointestinal nematodes also involves production of parasite-specific immunoglobulin A (IgA), IgG1 and IgE (Pfeffer et al, 1996;Kooyman et al, 1997;Shaw et al, 1998).…”

Section: Introductionmentioning

confidence: 99%

Serum antibody responses in Creole kids experimentally infected with Haemonchus contortus

Bambou¹,

Chevrotière²,

Varo³

et al. 2008

Veterinary Parasitology

View full text Add to dashboard Cite

show abstract

“…Recognition proteins such as lectins are involved in the immune response against nematodes (MEEUSEN et al, 2005). In particular, members of the galectin and the C-type lectin family have been identified in the abomasum of cattle and sheep infected with Ostertagia ostertagi and Cooperia oncophora (DUNPHY et al, 2000;LI & GASBARRE, 2009;LI et al, 2010;ROBINSON et al, 2011).…”

Section: Introductionmentioning

confidence: 99%

Galectins and collectinis expression are increased in Haemonchus contortus-infected corriedale sheep

Souza

Lambert

Nishi

et al. 2015

Rev. Bras. Parasitol. Vet.

View full text Add to dashboard Cite

Galectins and collectins are proteins classified in the lectin family that have the ability to recognize molecular patterns associated with pathogens. Studies on cattle have demonstrated high expression of these proteins during infection with gastrointestinal nematodes. The aim of this study was to investigate whether the level of Haemonchus contortus infection would alter the expression of galectins (Gal11 and Gal14) and collectins (SPA and CGN) in sheep. Twelve Corriedale sheep exposed to natural infection with nematodes were divided into two groups: group 1 (G1, n = 7) and group 2 (G2, n = 5), with low and high parasite burdens, respectively, based on fecal egg counts and abomasal parasite counts. The fecal egg counts and abomasal parasite counts were significantly different (p < 0.05) between the groups. Galectin and collectin gene expression was observed in all sheep abomasal samples. However, animals with lower infection levels showed lower expression of the genes Gal14, SPA and CGN (p < 0.05). Expression of lectins was associated with the abomasal H. contortus burden, thus suggesting that these proteins may have a role in controlling of this infection.Keywords: Innate immunity, lectins, helminths, small ruminants, PCR. ResumoColectinas e galectinas são proteínas da família das lectinas que possuem a capacidade de reconhecer padrões moleculares associados aos patógenos. Estudos em bovinos têm demonstrado a alta expressão dessas proteínas durante a infecção por nematoides gastrintestinais. O objetivo deste estudo foi investigar se o nível de infecção de Haemonchus contortus altera a expressão de colectinas (SPA e CGN) e galectinas (Gal11 e Gal14) de ovinos. Doze ovinos da raça Corriedale expostos a infecção natural com nematoides foram separados em dois grupos: grupo 1 (G1, n=7) com menor grau de parasitismo; e grupo 2 (G2, n=5) com maior grau, a partir da contagem do número de parasitos recuperados do abomaso e OPG. A contagem de OPG e de parasitos recuperados do abomaso dos grupos G1 e G2 apresentaram diferença estatística (p<0,05). A expressão dos genes de colectinas e galectina foi observada em todas as amostras de abomaso dos ovinos, porém animais com menor grau de infecção apresentaram menor expressão dos genes de Gal14, SPA e CGN (p<0,05). A expressão de lectinas foi associada ao número de H. contortus encontrados no abomaso de ovinos, indicando um possível papel dessas proteínas no controle da infecção.Palavras-chave: Imunidade inata, lectinas, helmintos, pequenos ruminantes, PCR.

show abstract

“…In particular, ruminants highly upregulate two specific galectins upon infection by various parasites such as Ostertagha, Cooperha and H. contortus (Dunphy et al 2000;Dunphy et al 2002;Hoorens et al 2011;Meeusen et al 2005).…”

Section: Ietroductioementioning

confidence: 99%

“…Although the L4 stage is a histotropic stage (in glands of the stomach) and would be expected to be in intimate contact with inflammatory mediators, including galectins, it moults (with a change in antigenic profile) within 48-72 h into the immature adult (Meeusen et al 2005). This would be expected to limit the antigenic exposure of these parasite antigens to the host.…”

Section: Larval Aed Adult Ligaeds Of Lgals-11 Aed Lgals-14mentioning

confidence: 99%

Proteomic identification of Galectin-11 and 14 ligands from Haemonchus contortus

Sakthivel

Swan

Preston

et al. 2017

Preprint

View full text Add to dashboard Cite

Haemonchus contortus is the most pathogenic nematode of small ruminants. Infection in sheep and goats results in anaemia that decreases animal productivity and can ultimately cause death. The involvement of ruminant-specific galectin-11 (LGALS-11) and galectin-14 (LGALS-14) has been postulated to play important roles in protective immune responses against parasitic infection; however, their ligands are unknown. In the current study,LGALS-11 and LGALS-14 ligands in H. contortus were identified from larval (L4) and adult parasitic stages extracts using immobilised LGALS-11 and LGALS-14 affinity column chromatography and mass spectrometry. AbstractHaemonchus contortus is the most pathogenic nematode of small ruminants. Infection in sheep and goats results in anaemia that decreases animal productivity and can ultimately cause death.The involvement of ruminant-specific galectin-11 (LGALS-11) and galectin-14 (LGALS-14) has been postulated to play important roles in protective immune responses against parasitic infection; however, their ligands are unknown. In the current study, ligands in H. contortus were identified from larval (L4) and adult parasitic stages extracts using immobilised LGALS-11 and LGALS-14 affinity column chromatography and mass spectrometry.

show abstract

Cells, cytokines and other molecules associated with rejection of gastrointestinal nematode parasites

Cited by 127 publications

References 15 publications

Serum antibody responses in Creole kids experimentally infected with Haemonchus contortus

Serum antibody responses in Creole kids experimentally infected with Haemonchus contortus

Galectins and collectinis expression are increased in Haemonchus contortus-infected corriedale sheep

Proteomic identification of Galectin-11 and 14 ligands from Haemonchus contortus

Contact Info

Product

Resources

About