Cells Involved in Urethral Tissue Engineering: Systematic Review

Culenova, Martina; Žiaran, Stanislav; Danišovič, Ľuboš

doi:10.1177/0963689719854363

Cited by 19 publications

(12 citation statements)

References 39 publications

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“…Results from the study of Orabi and De Filippo demonstrated that collagen scaffolds seeded with cells can be used for longer urethral replacement [ 12 , 18 ]. Therefore, the scaffolds and seeded-cells are the two critical components of tissue engineering urethra [ 19 – 21 ]. A good blood supply and sterile environment, which can only be provided in good animal models, are essential for the survival both of the scaffolds and the seeded-cells after its transplantation.…”

Section: Discussionmentioning

confidence: 99%

Comparison of two different methods of establishment of canine urethroplasty model: an experimental trial

et al. 2021

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Background Graft substitute urethroplasty is recommended for patients with long segment anterior urethral stricture. The therapeutic effects of the grafts need to be validated on the animal models. Therefore the aim of this study was to compared the operative time, blood loss, intra- and post- operative complications of two different methods of establishment of canine urethroplasty model. Methods Twelve Beagle dogs were randomly separated into control and experimental group using a random number table. Six animals in the control group received the conventional urethroplasty, while the other 6 in the experimental group received the modified procedures. Tube cystostomy and urethroplasty were performed in the control group. The cystostomy not the tube cystostomy were performed in the experimental group, and the testes were simultaneously removed with the scrotum. Per- and postoperative outcomes, complications were evaluated. Results The urethroplasty were successfully performed for all dogs and all of these procedures were done by the same surgeon. The median operative time in the control and experimental groups was 186.8 min and 188.7 min respectively. The blood loss in the control and experimental groups was 40.8 ml and 45.8 ml respectively. No intraoperative complications occurred. 3 animals in the control group developed acute urinary retention after the accidental removal of suprapubic bladder tube and the cystostomy was done again. There was no occurrence of urinary retention in the experimental group. 4 animals in the control group developed the perineal hematoma, in which one animal had the urine leakage and incision infection. Perineal hematoma occurred in only one animal in the experimental group. Conclusion The occurrence of urinary retention and perineal hematoma decreased in the modified group, in which the cystostomy not the tube cystostomy were performed and the testes with the scrotum were simultaneously removed.

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Section: Discussionmentioning

confidence: 99%

Comparison of two different methods of establishment of canine urethroplasty model: an experimental trial

et al. 2021

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“…They were followed up from 36 to 76 months postoperatively. Serial radiography and endoscopy revealed a wide, patent urethra with a median end maximum urinary flow rate of 27.1 ml/s (range [16][17][18][19][20][21][22][23][24][25][26][27][28]. Urethral biopsies at 3 months postimplantation revealed a normal histological architecture [45].…”

Section: Key Pointsmentioning

confidence: 98%

Tissue engineering: recent advances and review of clinical outcome for urethral strictures

Saad

Osman

Chapple

2021

Current Opinion in Urology

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Purpose of review Urethrotomy remains the first-line therapy in the treatment of a urethral stricture despite data showing no real chance of a cure after repeated urethrotomies. An anastomotic or an augmentation urethroplasty using oral mucosa can be offered to patients following failed urethrotomy. The potential for a tissue engineered solution as an alternative to native tissue has been explored in recent years and is reviewed in this article. Recent findings More than 80 preclinical studies have investigated a tissue-engineered approach for urethral reconstruction mostly using decellularized natural scaffolds derived from natural extracellular matrix with or without cell seeding. The animal models used in preclinical testing are not representative of disease processes seen with strictures in man. The available clinical studies are based on small noncontrolled series. Summary There is a potential role for tissue engineering to provide a material for substitution urethroplasty and work has demonstrated this. Further work will require a rigorous basic science programme and adequate evaluation of the material prior to its introduction into clinical practice. The research with tissue engineering applied to the urethra has not yet been resulted in a widely available material for clinical use that approaches the efficacy seen with the use of autologous grafts.

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“…Scholars have made many attempts to select scaffold materials and cells to achieve this goal. Nevertheless, the macro-and microstructure of the scaffold also plays a crucial role in urethral regeneration [17][18][19]. In this review, we divide urethral grafts into two types according to their structures: scaffold-free and scaffolded grafts.…”

Section: Biomimetic Grafts For Urethral Reconstructionmentioning

confidence: 99%

Tailor-made natural and synthetic grafts for precise urethral reconstruction

Tan

Tang

et al. 2022

J Nanobiotechnol

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Injuries to the urethra can be caused by malformations, trauma, inflammation, or carcinoma, and reconstruction of the injured urethra is still a significant challenge in clinical urology. Implanting grafts for urethroplasty and end-to-end anastomosis are typical clinical interventions for urethral injury. However, complications and high recurrence rates remain unsatisfactory. To address this, urethral tissue engineering provides a promising modality for urethral repair. Additionally, developing tailor-made biomimetic natural and synthetic grafts is of great significance for urethral reconstruction. In this work, tailor-made biomimetic natural and synthetic grafts are divided into scaffold-free and scaffolded grafts according to their structures, and the influence of different graft structures on urethral reconstruction is discussed. In addition, future development and potential clinical application strategies of future urethral reconstruction grafts are predicted.

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Cells Involved in Urethral Tissue Engineering: Systematic Review

Cited by 19 publications

References 39 publications

Comparison of two different methods of establishment of canine urethroplasty model: an experimental trial

Comparison of two different methods of establishment of canine urethroplasty model: an experimental trial

Tissue engineering: recent advances and review of clinical outcome for urethral strictures

Tailor-made natural and synthetic grafts for precise urethral reconstruction

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