Cells to Surgery Quiz: April 2021

Abstract

The overall incidence rate is higher in patients with hematologic malignancies. b. Immunopositivity for p63 has been shown to positively predict survival. c. Merkel cell polyomavirus seropositivity at diagnosis is associated with a decreased risk of recurrence. d. A wide local excision with at least 1e2 cm margins of normal-appearing skin is the preferred initial treatment modality for early-stage MCC.e. Avelumab and pembrolizumab are the United States Food and Drug Administration-approved treatments for advanced or metastatic MCC. 3. Song et al. (2021) performed single-cell RNA sequencing (scRNA-seq) in the classical MCC cell line WaGa after treatment with domatinostat and subsequent functional assays in additional MCC cell lines to determine its efficacy and mode of action in MCC. All of the following are consistent with their findings, except a. scRNA-seq of WaGa revealed that cells treated with domatinostat demonstrated significant differential expression of genes involved in biological processes and molecular functions. b. After treatment with domatinostat, viable cells were observed to have a strong induction of antigen-processing machinery component gene expression. c. Domatinostat was more effective than nocodazole in inducing both GM2 cell-cycle arrest and apoptosis. d. The observed effects of domatinostat were specific to MCC cells. e. Cells treated with domatinostat demonstrated an increase in HLA class I surface receptors in MCC cells in a dose-dependent manner.See following pages for detailed answers.