2019
DOI: 10.1016/j.bpj.2019.01.034
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Cells Under Stress: An Inertial-Shear Microfluidic Determination of Cell Behavior

Abstract: The deformability of a cell is the direct result of a complex interplay between the different constituent elements at the subcellular level, coupling a wide range of mechanical responses at different length scales. Changes to the structure of these components can also alter cell phenotype, which points to the critical importance of cell mechanoresponse for diagnostic applications. The response to mechanical stress depends strongly on the forces experienced by the cell. Here, we use cell deformability in both s… Show more

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Cited by 85 publications
(101 citation statements)
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“…, which was identified as the yield stress of the cells and is associated to cytoskeletal breakdown 32 Figure S1 shows the unnormalized data, DI as a function of Q, for the three cell lines in both shear and inertia-dominant regimes.…”
Section: Resultsmentioning
confidence: 99%
See 2 more Smart Citations
“…, which was identified as the yield stress of the cells and is associated to cytoskeletal breakdown 32 Figure S1 shows the unnormalized data, DI as a function of Q, for the three cell lines in both shear and inertia-dominant regimes.…”
Section: Resultsmentioning
confidence: 99%
“…Figure S2 shows the velocity profiles calculated along the central axis of the extensional flow junction, that were fitted with a sine function and www.nature.com/scientificreports www.nature.com/scientificreports/ used to fit the Kelvin-Voigt model to the deformation trace (shown in red in Figure S2). As a comparison, control data from the averaged deformation trace of N = 50 HL60 cells is also included in Table 1 from Armistead et al (2019) 32 .…”
Section: Resultsmentioning
confidence: 99%
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“…Cross-slot microfluidic deformability cytometry (mDC) is a promising high-throughput approach for mechanotypic screening of living cells, but it is plagued with measurement errors associated with cell offset from the flow centerline (24,37) and pressure or velocity fluctuations at the inlet channels. One of the ways to reduce offset errors is to eliminate the off-distance deformation index (DI) data (20,23), which can make mDC less powerful in assessing changes in cell mechanotype. We have demonstrated via predictive computational modeling that the mDC output can be sensitized to mechanical properties of the cells such as shear elasticity without removing viable off-distance data.…”
Section: Discussionmentioning
confidence: 99%
“…Simple analytical expressions 4 that relate the DI with channel geometry and cell's viscoelastic properties based on Maxwell and Kevin-Voigt models exist only for the cell moving along the flow centerline under low-velocity, Stokes flow conditions (21)(22)(23)(24). Commercial software packages (e.g., COMSOL Multiphysics ® ) that can only simulate flow but not cell deformation in a cross-slot channel have been employed to optimize the channel geometry for mDC (20,21,(25)(26)(27). This approach worked well for Re ≤ 10, but failed for higher Re.…”
Section: Introductionmentioning
confidence: 99%