Cellular activities during a mixed leucocyte reaction in the teleost sea bass Dicentrarchus labrax

Conservation of characteristics and functions of CD4 positive lymphocytes in a teleost fish

“…MLC/MLR has been reported in several fish species upon in vitro incubation of allogeneic leukocytes (see Meloni et al, 2006). In channel catfish it has been reported that surface Ig-negative (sIg − ) lymphocytes were the responding cells in MLC (Miller et al,Fig.…”

Cellular and molecular immune responses of the sea bass (Dicentrarchus labrax) experimentally infected with betanodavirus

“…Cell proliferation was determined by assaying intracellular ATP levels of leucocyte cultures using a luminometric assay (ATP-lite, Perkin Elmer) as previously described [26,27]. Briefly, 100 ml of culture medium containing 5 Â 10 5 leucocytes from control and VERv-treated fish were incubated for 48 h in L15 medium containing 5% FCS, antibiotics, and where required with 5 ml of the same VERv preparation obtained as above described, but inactivated by heat treatment (65 C, 20 min).…”

“…1. Cell proliferation was measured by the quantitative determination of intracellular ATP, already shown to be related to cell viability and cell number in sea bass [27]. The bars show the content of intracellular ATP measured in cultures of leucocytes coming from samples inoculated with PBS (black bars), and from samples inoculated with VERv (grey bars).…”

Molecular cloning and characterization of carp (Cyprinus carpio L.) CD8β and CD4-like genes

“…In vitro, some of the alloreactive TCR þ channel catfish cytotoxic cell clones kill their targets [37], specific cell-mediated cytotoxicity (CMC) against allogeneic target cells has also been shown in rainbow trout [38], thus providing further evidence on the existence of cytotoxic T lymphocytes (CTL) in teleosts. In 2005, Meloni et al also confirmed the ''in vitro'' activities of T cells in teleost sea bass by mixed leukocyte reaction (MLR) and RT-PCR to analysis the expression of the T cell receptor (ß-subunit) mRNA [39]. Moreover, many evidences of ''in vivo'' activities of CTL have been shown in teleost species such as sea bass [40], ginbuna carp [41] and amago salmon (Oncorhynchus rhodurus) [42].…”