Cellular alterations in collembolan midgut cells as a marker of heavy metal exposure: ultrastructure and intracellular metal distribution

Pawert, Michael; Triebskorn, Rita; Gräff, Sybille; Berkus, Martin; Schulz, Jörn; Köhler, Heinz‐R.

doi:10.1016/0048-9697(95)05009-4

Cited by 67 publications

(24 citation statements)

References 29 publications

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“…Despite its capacity to store a variety of metals, this intracellular system of detoxification may be overcharged by high metal concentrations in the animal's diet. In an ecotoxicological study, Pawert et al (1996) showed by means of EELS and ESI that excessive amounts of zinc may enter the membranes of mitochondria and microvilli of the midgut epithelial cells (Fig. 4).…”

Section: Springtails (Collembola)mentioning

confidence: 99%

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Localization of metals in cells of saprophagous soil arthropods (Isopoda, Diplopoda, Collembola)

Köhler¹

2002

Microscopy Res & Technique

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This review summarizes results on the intracellular distribution of metals in cells of woodlice (Isopoda), millipedes (Diplopoda), and springtails (Collembola), which are three major groups of saprophagous arthropods contributing to the turnover of soil organic matter. Although the impact of metals and also metal pollution has inevitably been shown at levels of higher biological organization than subcellular mechanisms in these animal groups, the aim of this review is to focus exclusively on storage sites and aspects of intracellular metal metabolism. Thus, methodologically, results obtained by microscopical techniques such as histochemistry, X-ray microanalysis, energy filter transmission electron microscopy, or laser microprobe mass spectrometry were given preference. Results from atomic absorption spectrophotometry of cellular fractions were kept to a minimum. In all three taxa, the main intracellular metal storage sites are various types of "granules" which are widely distributed throughout cell types associated with the digestive system.

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Section: Springtails (Collembola)mentioning

confidence: 99%

“…In their typical shape type A granules have been detected, e.g., in the epithelial layers of the midgut resorptive tissue of Diplopoda and Collembola (Humbert, 1974(Humbert, , 1977(Humbert, , 1978(Humbert, , 1979van Straalen et al, 1987;Pawert et al, 1996). In isopods, type A granules have not been detected (Hopkin, 1989).…”

Section: Introductionmentioning

confidence: 95%

Localization of metals in cells of saprophagous soil arthropods (Isopoda, Diplopoda, Collembola)

Köhler¹

2002

Microscopy Res & Technique

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“…The decrease in hsp70 expression is partly due to strong damage of the cells' protein synthesis apparatus required for hsp70 synthesis (Meyer et al, 1995). Marked pathological damage is also partly responsible for the reduction in hsp70 (Pawert et al, 1996;. Cytotoxic effects like membrane damage lead to decreasing hsp70 contents compared to control groups .…”

Section: Accepted Manuscriptmentioning

confidence: 99%

Expression of heat shock protein 70 in a permanent cell line (EPC) exposed to sediment extracts from the North Sea and the Baltic Sea

Kinder

Sierts-Herrmann

Biselli

et al. 2007

Marine Environmental Research

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“…In the springtail Tetrodontophora bielanensis exposure to Cd, Pb and Zn led to a number of cellular rearrangements, particularly an increase of the number of granules of type-A (rich in Ca and P), which contained a large fraction of the cellular heavy metals (Pawert et al, 1996). Granules of type B, which are rich in S and organic matter, are supposed to be associated with the MT detoxification system, but have not yet been observed in Collembola (Köhler, 2002).…”

Section: The Orchesella Cincta Cadmium Tolerance Case Studymentioning

confidence: 99%

Molecular mechanisms of heavy metal tolerance and evolution in invertebrates

Janssens¹,

Roelofs²,

Straalen³

2009

Insect Science

149

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Following the genomics revolution, our knowledge of the molecular mechanisms underlying defenses against stress has been greatly expanded. Under strong selective pressure many animals may evolve an enhanced stress tolerance. This can be achieved by altering the structure of proteins (through mutations in the coding regions of genes) or by altering the amount of protein (through changes in transcriptional regulation). The latter type of evolution can be achieved by substitutions in the promoter of the gene of interest (cisregulatory change) or by altering the structure or amount of transcriptional regulator proteins (trans-regulatory change). The metallothionein system is one of the best studied stress response systems in the context of heavy metals. Metallothionein expression is assumed to be regulated by metal transcription factor 1 (MTF-1); however, up to now the involvement of MTF-1 has only been proven for some vertebrates and Drosophila. Data on invertebrates such as nematodes and earthworms suggest that other mechanisms of metallothionein induction may be present. A detailed study of Cd tolerance was done for a species of soilliving springtail, Orchesella cincta. The metallothionein gene of this species is overexpressed in metal-exposed field populations. Analysis of the metallothionein promoter has demonstrated extensive polymorphisms that have a functional significance, as shown in bioreporter assays. In a study comparing 20 different populations, the frequency of a high-expresser promoter allele was positively correlated with the concentration of metals in soil, especially Cd. The springtail study shows that cis-regulatory change of genes involved in the cellular stress response may contribute to evolution of metal tolerance.

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Cellular alterations in collembolan midgut cells as a marker of heavy metal exposure: ultrastructure and intracellular metal distribution

Cited by 67 publications

References 29 publications

Localization of metals in cells of saprophagous soil arthropods (Isopoda, Diplopoda, Collembola)

Localization of metals in cells of saprophagous soil arthropods (Isopoda, Diplopoda, Collembola)

Expression of heat shock protein 70 in a permanent cell line (EPC) exposed to sediment extracts from the North Sea and the Baltic Sea

Molecular mechanisms of heavy metal tolerance and evolution in invertebrates

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