Cellular analysis of UV-B-induced barley root subapical swelling

Ktitorova, I. N.; Demchenko, N. P.; Kalimova, I. B.; Demchenko, Kirill N.; Skobeleva, O. V.

doi:10.1134/s1021443706060148

Cited by 14 publications

(13 citation statements)

References 26 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…1) and alterations of primary root morphology (Fig. 2) in A. thaliana are consistent with findings of other researchers (Zhang et al 2003, Ktitorova et al 2006). First, alterations in the cell differentiation status as ectopic root hair formation in swollen epidermal cells were noticed (Fig.…”

Section: Discussionsupporting

confidence: 92%

Nitric oxide as a critical factor for perception of UV‐B irradiation by microtubules in Arabidopsis

Krasylenko

Yemets

Sheremet

et al. 2011

Physiologia Plantarum

View full text Add to dashboard Cite

Influence of ultraviolet-B (UV-B) as an abiotic stress factor on plant microtubules (MTs) and involvement of nitric oxide (NO) as a secondary messenger mediating plant cell response to environmental stimuli were investigated in this study. Taking into account that endogenous NO content in plant cells has been shown to be increased under a broad range of abiotic stress factors, the effects of UV-B irradiation and also the combined action of UV-B and NO donor sodium nitroprusside (SNP) or NO scavenger 2-(4-carboxyphenyl)-4,4,5,5-tetramethylimidazoline-1-oxyl-3-oxide (c-PTIO) on the MTs organization in different root cells of Arabidopsis thaliana were tested. Subsequently, realization of the MT-mediated processes such as root growth and development was studied under these conditions. Arabidopsis thaliana seedlings expressing the chimeric gene gfp-map4 were exposed to the enhanced UV-B with or without SNP or c-PTIO pretreatment. The UV-B irradiation alone led to a dose-dependent root growth inhibition and to morphological alterations of the primary root manifested in their swelling and excessive root hair formation. Moreover, dose-dependent randomization and depolymerization of MTs in both epidermal and cortical cells under the enhanced UV-B were found. However, SNP pretreatment of the UV-B irradiated A. thaliana seedlings recovered the UV-B inhibited root growth as compared to c-PTIO pretreatment. It has been shown that in 24 h after UV-B irradiation the organization of MTs in root epidermal cells of SNP-pretreated A. thaliana seedlings was partially recovered, whereas in c-PTIO-pretreated ones the organization of MTs has not been distinctly improved. Therefore, we suppose that the enhanced NO levels in plant cells can protect MTs organization as well as MT-related processes of root growth and development against disrupting effects of UV-B.

show abstract

Section: Discussionsupporting

confidence: 92%

Nitric oxide as a critical factor for perception of UV‐B irradiation by microtubules in Arabidopsis

Krasylenko

Yemets

Sheremet

et al. 2011

Physiologia Plantarum

View full text Add to dashboard Cite

show abstract

“…3a) can be only explained by the temporal decrease in Π in . We showed in previous studies that Π in was eventually restored and even exceeded the control level in 6 h after irradiation [8]. The successive decrease and restoration of turgor might account for nonmonotonic changes in L p and δD/D under sever stress.…”

Section: Possible Causes Of L P and δD/d Dynamics Under Severe Stressmentioning

confidence: 85%

“…Our previous studies showed that retardation of root growth upon UV B irradiation of barley shoots or roots is also associated with the appearance of oxida tive stress in the root growth zone and with stiffening of cell walls [7,8]. Another possible cause of growth reduction is a Ca 2+ dependent disorganization in the array of cortical microtubules (CMT) induced by the presence of aluminum or glutamate in the medium [9] and by acidification of the external solution [10].…”

Section: Introductionmentioning

confidence: 98%

Biophysical parameters as informative tools for elucidating the causes of root growth retardation under stressful conditions

Ktitorova

Skobeleva

Agal’tsov

2011

Russ J Plant Physiol

Self Cite

View full text Add to dashboard Cite

The root growth rate in barley (Hordeum vulgare L.) seedlings was measured in parallel with tem poral changes in longitudinal (δl) and transverse (δD/D) cell wall extensibilities and membrane hydraulic conductivity (L p ) in the root extension zone. The root growth rate and biophysical parameters examined were sensitive to UV B irradiation of shoots or roots and to excessive content of ammonium, glutamate, or nickel in the nutrient medium. The root responses to the above treatments were compared with the effects of abscisic acid, salicylate, hydrogen peroxide, diethylstilbestrol, α naphthyl acetate, oryzalin, and ionomycin. The pro gressive reduction of root growth under the action of various stressors was accompanied by typical temporal patterns of the growth zone parameters: the δl extensibility declined monotonically, while δD/D and L p changed nonmonotonically, exhibiting the reversion from the initial decrease to the eventual increase above the control values. The decline of δl indicated that the root growth suppression was mainly due to changes in cell wall mechanical properties caused probably by disorganization of cortical microtubules. It was found that the decline in δD/D and L p was caused primarily by the appearance of oxidative stress, disorders in cyto plasmic H + homeostasis in root cells, and the consequent transient activation of the plasmalemmal H + pump. Conversely, the increase in δD/D and L p upon the abrupt retardation of root growth was presumably caused by the increase in cytoplasmic Ca 2+ content, disassembling of cortical microtubules, and by partial inhibition of the plasmalemmal H + pump. The reversion of δD/D and L p changes upon progressive reduction of root growth can be used as an indicator to distinguish moderate and severe stress conditions in the root growth zone. Furthermore, this reversion indicates the increasing disbalance in the homeostasis of reactive oxygen species, cytosolic Ca 2+ , and cytosolic H + upon severe stress. Abbreviations: CMT-cortical microtubules; D-root diameter; DES-diethylstilbestrol; and -cytosolic concen trations of H + and Ca 2+ ; L p -hydraulic conductivity of the rhizodermal cell membrane; δD/D-transverse extensibility of the root; δl-longitudinal extensibility of the root; Π in -internal osmotic pressure in the root; Π o -osmotic pressure of the medium. H cyt + Ca cyt 2+ RESEARCH PAPERS

show abstract

“…However, these alterations in microtubule organization and the arrest of cell division observed in vitro were not revealed in the epidermal cells of P. hybrida leaves grown under UV-B radiation (9 kJ\m 2 day) (Staxèn and Bornman 1994). Cytoskeleton rearrangements are supposed to underlie UV-B-induced changes of anisotropic mechanical properties of barley (Hordeum vulgare L.) cell walls and root hair initiation, since 100 µM colchicine treatment resulted in root swelling (Ktitorova et al 2006). Later, it was shown that the intense UV-B exposure (10.08 kJ/m 2 day) caused stick-and-spot depolymerization of microtubules in T. aestivum L. mesophyll protoplasts (Guo et al 2010).…”

Section: The Role Of No-dependent Regulatory Cascades In Uv-b Perceptmentioning

confidence: 99%

Nitric Oxide and UV-B Radiation

Yemets

Krasylenko

Blume

2015

Nitric Oxide Action in Abiotic Stress Responses in Plants

View full text Add to dashboard Cite

Over the past few years, nitric oxide (NO) has emerged as a key player in plant adaptive/hypersensitive responses to abiotic (stress) factors, since the content of endogenously generated NO increases in adverse environmental conditions. This chapter addresses to the signalling events with the involvement of the reactive nitrogen species under ultraviolet-B (UV-B) exposure. Solar UV-B has long been recognized as merely deleterious environmental factor; however, rapidly increasing evidence indicates that it plays regulatory role as photomorphogenic trigger for plants. In turn, NO is supposed to be the crucial signalling molecule under plant response to UV-B. The putative biochemical mechanisms of the protective effects of exogenous NO donors on plant cells and the input of NO-synthase-like activities and nitrate reductase under UV-B exposure are discussed. The involvement of cytoskeleton-related NO signalling pathways (especially based on involvement) of microtubules in realization of intracellular UV-B effects is highlighted in the review.

show abstract

Cellular analysis of UV-B-induced barley root subapical swelling

Cited by 14 publications

References 26 publications

Nitric oxide as a critical factor for perception of UV‐B irradiation by microtubules in Arabidopsis

Nitric oxide as a critical factor for perception of UV‐B irradiation by microtubules in Arabidopsis

Biophysical parameters as informative tools for elucidating the causes of root growth retardation under stressful conditions

Nitric Oxide and UV-B Radiation

Contact Info

Product

Resources

About