Cellular and Acellular Approaches for Cartilage Repair

Brittberg, Mats

doi:10.1177/1947603514536983

Cited by 6 publications

(7 citation statements)

References 54 publications

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“…A meta-analysis including 117 studies by Pot et al indicated that although results varied between different cell sources, the results of the applications including cells were superior to application without cells [43]. As also stated by an important author of autologous chondrocyte implantation Brittberg, cells are always necessary to repair a damaged tissue in terms of biology [44]. The groups of this study designed under the light of this information only include treatments with cells which were shown to be superior compared to the alternatives without cells.…”

Section: Discussionmentioning

confidence: 99%

Autologous stem cell-derived chondrocyte implantation with bio-targeted microspheres for the treatment of osteochondral defects

Bozkurt¹,

Aşık²,

Gürsoy³

et al. 2019

J Orthop Surg Res

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BackgroundChondral injury is a common problem around the world. Currently, there are several treatment strategies for these types of injuries. The possible complications and problems associated with conventional techniques lead us to investigate a minimally invasive and biotechnological alternative treatment. Combining tissue-engineering and microencapsulation technologies provide new direction for the development of biotechnological solutions. The aim of this study is to develop a minimal invasive tissue-engineering approach, using bio-targeted microspheres including autologous cells, for the treatment of the cartilage lesions.MethodIn this study, a total of 28 sheeps of Akkaraman breed were randomly assigned to one of the following groups: control (group 1), microfracture (group 2), scaffold (group 3), and microsphere (group 4). Microspheres and scaffold group animals underwent adipose tissue collection prior to the treatment surgery. Mesenchymal cells collected from adipose tissue were differentiated into chondrocytes and encapsulated with scaffolds and microspheres. Osteochondral damage was conducted in the right knee joint of the sheep to create an animal model and all animals treated according to study groups.ResultsBoth macroscopic and radiologic examination showed that groups 3 and 4 have resulted better compared to the control and microfracture groups. Moreover, histologic assessments indicate hyaline-like cartilage formations in groups 3 and 4.ConclusionIn conclusion, we believe that the bio-targeted microspheres can be a more effective, easier, and safer approach for cartilage tissue engineering compared to previous alternatives.

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Section: Discussionmentioning

confidence: 99%

Autologous stem cell-derived chondrocyte implantation with bio-targeted microspheres for the treatment of osteochondral defects

Bozkurt¹,

Aşık²,

Gürsoy³

et al. 2019

J Orthop Surg Res

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“…Cartilage regeneration techniques rely primarily in cell therapy, with the first technique describing autologous chondrocyte implantation. 2 The best results have been accomplished with the use of chondrocytes for cartilage restoration, since the ultimate goal is hyaline cartilage. 2 Despite progress been made, new cell sources are being researched, namely, bone marrow-derived hMSCs.…”

Section: Introductionmentioning

confidence: 99%

“…2 The best results have been accomplished with the use of chondrocytes for cartilage restoration, since the ultimate goal is hyaline cartilage. 2 Despite progress been made, new cell sources are being researched, namely, bone marrow-derived hMSCs. These must express the following cell surface receptors in at least 95%: CD105, CD73, and CD90; and be negative for CD45, CD14, CD34, CD19, and Class II HLA.…”

Section: Introductionmentioning

confidence: 99%

“…4 Also, several studies have seen deterioration of this neocartilage after a median of 18 months. 2,5 In the past years another approach to regenerate hyaline cartilage for articular purposes has focused on the use of stem cells from several origins, and commitment for having a developmental recapitulation of cartilage in vitro. In order to do so, developmental gene regulation must be taken into account.…”

Section: Introductionmentioning

confidence: 99%

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SERPINA9 and SERPINB2: Novel Cartilage Lineage Differentiation Markers of Human Mesenchymal Stem Cells with Kartogenin

Granados-Montiel

Cruz‐Lemini²,

Rangel‐Escareño

et al. 2018

CARTILAGE

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Objective Human mesenchymal stem cells (hMSCs) are a promising source for regenerative medicine, especially mesodermal lineages. Clinical applications require an understanding of the mechanisms for transcriptional control to maintain the desired cell type. The aim of this study was to identify novel markers for differentiation of hMSCs into bone or cartilage with the use of Kartogenin, by RNA analysis using microarray technology, and explore the role of RhoA-Rho associated protein kinase (ROCK) inhibition in these. Methods Commercial human bone marrow derived primary mesenchymal stem cells were purchased from ATCC. Cells were differentiated in vitro in 2-dimensional cultures using Kartogenin as the main cartilage inducer and bone morphogenetic protein 2 for bone differentiation; cells were cultured with and without ROCK inhibitor Y-27632. After 21 days of culture, whole RNA was extracted and analyzed via Affimetrix microarrays. The most significant hits were validated by quantitative polymerase chain reaction. Results We found a total of 1,757 genes that were either up- or downregulated on differentiation, when compared to P1 hMSC (control) at day 0 of differentiation. Two members of the Serpin superfamily, SERPINA9 and SERPINB2, were significantly upregulated in the cartilage groups, whereas they were unchanged in the bone groups with and without ROCK inhibition. Conclusions SERPINA9 and SERPINB2 are novel differentiation markers, and molecular regulator candidates for hMSC lineage commitment toward bone and cartilage, providing a new tool for regenerative medicine. Our study highlights the roles of these 2 genes, with significant upregulation of both in cell cultures stimulated with Kartogenin.

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“…As a consequence, there is an urgent need for interventions that can help to prevent these disorders and therapies to effectively treat them. To date, these objectives are far from being achieved and optimal cartilage reconstruction still represents an unmet clinical need [2,3]. Cell-based therapy for cartilage repair aims at not only filling the tissue defect with a substitute, but also reconstituting the structure, physicochemical properties, and functionality of the hyaline matrix, possibly promoting intimate integration with the resident tissue [4,5].…”

Section: Introductionmentioning

confidence: 99%

Emerging potential of gene silencing approaches targeting anti-chondrogenic factors for cell-based cartilage repair

Lolli

Penolazzi

Narcisi

et al. 2017

Cell. Mol. Life Sci.

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The field of cartilage repair has exponentially been growing over the past decade. Here, we discuss the possibility to achieve satisfactory regeneration of articular cartilage by means of human mesenchymal stem cells (hMSCs) depleted of anti-chondrogenic factors and implanted in the site of injury. Different types of molecules including transcription factors, transcriptional co-regulators, secreted proteins, and microRNAs have recently been identified as negative modulators of chondroprogenitor differentiation and chondrocyte function. We review the current knowledge about these molecules as potential targets for gene knockdown strategies using RNA interference (RNAi) tools that allow the specific suppression of gene function. The critical issues regarding the optimization of the gene silencing approach as well as the delivery strategies are discussed. We anticipate that further development of these techniques will lead to the generation of implantable hMSCs with enhanced potential to regenerate articular cartilage damaged by injury, disease, or aging.

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Cellular and Acellular Approaches for Cartilage Repair

Cited by 6 publications

References 54 publications

Autologous stem cell-derived chondrocyte implantation with bio-targeted microspheres for the treatment of osteochondral defects

Autologous stem cell-derived chondrocyte implantation with bio-targeted microspheres for the treatment of osteochondral defects

SERPINA9 and SERPINB2: Novel Cartilage Lineage Differentiation Markers of Human Mesenchymal Stem Cells with Kartogenin

Emerging potential of gene silencing approaches targeting anti-chondrogenic factors for cell-based cartilage repair

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