Cellular and collagen reference values of gingival and periodontal ligament tissues in rats: a pilot study

Tissue Engineering for Periodontal Ligament Regeneration: Biomechanical Specifications

“…The final biomaterial has to show biomechanical properties that allow its resistance during mastication [75,219]. The validation of structural properties of the PDL could be performed by comparing with reference values for collagen and periodontal cellular network as proposed by Alves et al [221]. [219].…”

“…Its mechanical fatigue should be evaluated within a realistic mechanical environment [219]. Finally, its behavior within a physiological environment can be evaluated using animal models [221]. It has not been discussed in the context of this review article, but the perfusion fluid composition and types of cell used for the development of such a biomaterial are also of major interest. Besides the technological challenge that is the development of an ideal biomaterial, the aim for the definition of such biomechanical specifications is to improve our scientific knowledge regarding periodontal regeneration.…”

Periodontal Destruction and Regeneration in Experimental Models: Combined Research Approaches

“…Nevertheless, according to Alves A. et al (2019) reports, there is a lack of evidence data from animal models, which would be useful for studies of new periodontal treatments [12]. Scaling and root planing may not always be effective in preventing periodontal disease progression, and, moreover, with currently available therapies, full regeneration of lost periodontal tissues after periodontitis cannot be achieved [13,14].…”

“…The results obtained were the subject of histomorphomorphological/-metrical [10, 13, 14, 17, 19, 20, 21-25, 27-29, 30], immunohisto (-cyto)chemical [15,16,10,20,7,28,21,29,6,30,5,19] and histopathological [2,4,5,9,31,22] analysis, micro-computed tomography [9,15,16,18,19,21,24,28]. The laboratory methods involved were real-time quantitative reverse-transcription polymerase chain reaction [11,15,16,21] enzyme-linked immunosorbent assay [16,17], scanning [3], and transmission electron microscopy [32], polarizing light microscopy [29], confocal microscopy and spectrophotometry [15], radiographic and biomechanical analysis [18], descriptive histology and computer-assisted image analysis [12].…”

“…The structural and compositional computerized assessment performed in the healthy periodontal soft tissue could provide reference values that will be required for future assessment on the effects of pathological, reparative and regenerative processes in rat periodontal soft tissues. The mild decalcification method allowed Alves A. et al (2019) in situ maintenance of the periodontal soft and hard tissue integrity, and histomorphometrical data showed that qualitatively, cellular and extracellular matrix morphologies were well preserved compared to non-decalcified periodontal soft tissue biopsies [12]. The effects of fluoxetine, a selective serotonin reuptake inhibitor, on the formation of the periodontal ligament collagen fibers in rats, and ipriflavone on new bone formation in the periodontal tissue in vivo, were assessed by Regueira L. S. et al (2017) and Han Y. et al [10,29].…”