Cellular and in vitro toxicity of nanodiamond-polyaniline composites in mammalian and bacterial cell

Villalba, Pedro; Ram, Manoj K.; Gómez, H.; Bhethanabotla, Venkat R.; Helms, My N.; Kumar, Amrita; Kumar, Ashok

doi:10.1016/j.msec.2011.12.017

Cited by 36 publications

(23 citation statements)

References 24 publications

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“…In the case of LM2 cells, the LC50 determined by MTT is 7.5 mg PANI-Nps/ mL; compared to the LC50 of 11.8 mg/mL determined by Neutral Red assay. These results were found at very high concentrations compared with other PANI nanomaterials with toxic effects at concentration starting of 1 and 100 lg/mL, respectively (Villalba et al 2012;Kucekova et al 2014). These differences could be attributed to modifications in nanoparticle's composition and properties like the shape and size.…”

Section: Biocompatibility Of Pani-npsmentioning

confidence: 65%

“…Polyaniline has a high optical absorbance in the NIR region, where NIR light achieves the highest tissue penetration. Moreover, polyaniline is noncytotoxic and has been successfully used as an electroactive material for studying cellular proliferation and in the field of nanomedicine (Li et al 2009;Yang et al 2011;Villalba et al 2012). In the present study, the ability of an organic conducting polymer to be employed in photothermal therapy is analyzed evaluating PANI-Nps toxicity, the cell uptake, and its ability to promote tumor cell death by photothermic effect, thus revealing a possible mechanism of cell death.…”

Section: Introductionmentioning

confidence: 99%

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Polyaniline nanoparticles for near-infrared photothermal destruction of cancer cells

et al. 2015

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Polyaniline nanoparticles (PANI-Nps) have been used in several applications; however, there are few publications related to the use in the photothermal therapy. PANI-Nps have high optical absorbance in the near-infrared region and in this wavelength range, biological systems are relatively transparent. For this reason, these materials can be used to absorb energy and to generate heat that destroys cancer cells selectively. PANI-Nps with average size of ca. 200 nm and neutral zeta potential were synthesized and characterized by DLS, SEM, and zeta potential. The kinetics of incorporation of PANINps into LM2 cell line was monitored using UV-Vis spectrophotometry. The analysis of cell viability after PANI-Nps exposure shows that these nanoparticles are not cytotoxic even at high concentration and show no change in cell morphology and metabolic activity. Furthermore, we found that nanoparticle cell uptake reaches the maximum value c.a. 3 h after incubation. Cells were targeted by Pani-Nps and irradiated, resulting in significant elevation of intracellular ROS and heat production. One of the mechanisms of PANINps-mediated photothermal killing of cancer cells apparently involved oxidative stress resulting in apoptotic cell death.

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Section: Biocompatibility Of Pani-npsmentioning

confidence: 65%

Section: Introductionmentioning

confidence: 99%

Polyaniline nanoparticles for near-infrared photothermal destruction of cancer cells

et al. 2015

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“…11 However, in their studies, the drug release in tumor microenvironment was not satisfied with our expectation and the antitumor activity of the complexes was no match for the free DOX. Nanodiamond (ND) has recently attracted significant attention in biological application, [12][13][14][15][16][17][18][19][20] owing to its better biocompatibility, optical properties, chemical stability and surface easily modified. [21][22][23][24][25][26][27][28][29][30][31] However, ND has a serious trend to aggregate to influence its application.…”

Section: Introductionmentioning

confidence: 99%

Smart pH-responsive and high doxorubicin loading nanodiamond for in vivo selective targeting, imaging, and enhancement of anticancer therapy

Tian

Wang

et al. 2016

J. Mater. Chem. B

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Nanodiamond as a carrier for transporting chemotherapy drugs has emerged as a promising strategy for treating cancer. However, several factors limited its extensive applications in biology, such as low drug loading, easily cluster and a high drug loss under physiological environment. In this work, to ensure high drug capacity and low drug leakage in physiological conditions, and especially ensure to be delivered to tumor region, a smart pH-responsive drug delivery system was designed and prepared by DOX adsorbed onto PEGylated nanodiamond in sodium citrate medium (ND-PEG-DOX/Na3Cit, NPDC). The system can significantly enhance cellular uptake to exert therapeutic effect in comparison to free drug. And more importantly, DOX was released in a sustained and pH-dependent manner and exhibits excellent stability under neutral conditions. In addition, NPDC could enter into cells via both clathrin and caveolae-mediated endocytosis pathway, and then dissociated DOX migrated into nucleus to block the growth of cancer cells. Furthermore, NPDC can significantly inhibit the cell migration and change the cell cycle. Excitingly, the NPDC system was very smart to enrich at the tumor site in vivo effectively and to enhance antitumor efficiency with low toxicity beyond conventional DOX treatment in cancer cells and nude mouse model. So this study introduces a simple and effective strategy to design a promising drug delivery platform for improving the biomedical applications of the smart nanodiamonds carriers. amount of loaded DOX is relative poor and the drug dissociation in physiological environment is slightly high. Scheme 1 Schematic illustration of the fabrication of an intracellular pHresponsive NPDC delivery system for cancer therapy.

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“…[81] Similarly to previously discussed types of functionalized NDs, ND-PANI displayed a dose-dependent trend of cytotoxicity when incubated with human embryonic kidney (HEK-293) cells in vitro . While lower concentrations (1 μg/mL) of ND-PANI had no adverse effects on the cells, higher concentrations (10 μg/mL) were reported to be cytotoxic and warrant further study before use.…”

Section: Synthesis and Functionalization Of Ndsmentioning

confidence: 75%

Multifunctional nanodiamonds in regenerative medicine: Recent advances and future directions

Whitlow

Pacelli

Paul

2017

Journal of Controlled Release

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With recent advances in the field of nanomedicine, many new strategies have emerged for diagnosing and treating diseases. At the forefront of this multidisciplinary research, carbon nanomaterials have demonstrated unprecedented potential for a variety of regenerative medicine applications including novel drug delivery platforms that facilitate the localized and sustained release of therapeutics. Nanodiamonds (NDs) are a unique class of carbon nanoparticles that are gaining increasing attention for their biocompatibility, highly functional surfaces, optical properties, and robust physical properties. Their remarkable properties have established NDs as an invaluable regenerative medicine platform, with a broad range of clinically relevant applications ranging from targeted delivery systems for insoluble drugs, bioactive substrates for stem cells, and fluorescent probes for long-term tracking of cells and biomolecules in vitro and in vivo. This review introduces the synthesis techniques and the various routes of surface functionalization that allow for precise control over the properties of NDs. It also provides an in-depth overview of the current progress made towards the use of NDs in the fields of drug delivery, tissue engineering, and bioimaging. Their future outlook in regenerative medicine including the current clinical significance of NDs, as well as the challenges that must be overcome to successfully translate the reviewed technologies from research platforms to clinical therapies will also be discussed.

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Cellular and in vitro toxicity of nanodiamond-polyaniline composites in mammalian and bacterial cell

Cited by 36 publications

References 24 publications

Polyaniline nanoparticles for near-infrared photothermal destruction of cancer cells

Polyaniline nanoparticles for near-infrared photothermal destruction of cancer cells

Smart pH-responsive and high doxorubicin loading nanodiamond for in vivo selective targeting, imaging, and enhancement of anticancer therapy

Multifunctional nanodiamonds in regenerative medicine: Recent advances and future directions

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