Cellular and Molecular Basis for Antigen Transport in the Intestinal Epithelium

Neutra, Marian R.; Kraehenbuhl, Jean–Pierre

doi:10.1016/b978-0-12-524730-6.50008-7

Cited by 8 publications

(1 citation statement)

References 135 publications

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“…The small intestine of the neonatal rat is structurally and functionally immature before weaning (1,2). This immaturity is characterized by increased permeability of the mucosal barrier, leading to enhanced uptake of macromolecules from the intestinallumen (3).…”

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confidence: 99%

Influence of the Polyamine, Spermidine, on Intestinal Maturation and Dietary Antigen Uptake in the Neonatal Rat

Capano,

Bloch,

Schiffrin

et al. 1994

J. pediatr. gastroenterol. nutr.

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SummaryPolyamines appear to have an important role in postnatal growth of the rat intestine. In the present study, we examined the effect of spermidine on the maturation of the intestine and on its ability to exclude macromolecules. Two litters of Sprague‐Dawley rat pups were assigned to one of four experimental groups. These groups received, on Days 7, 8, and 9, either (a) saline by gavage; (b) spermidine, 0.9 mg (6 μmol) by gavage; (c) cortisone acetate, 3.5 mg i.p.; or (d) saline i.p. On Day 10, animals were fed by gavage with a mixture of bovine serum albumin (BSA; 2 mg/g) and the γ‐globulin fraction of mouse antiovalbumin (anti‐OVA) antiserum (1 mg/g) and were bled 4 h later. Intestinal tissues were processed for histologic examination, sucrase determination, and identification of neonatal intestinal Fc receptor (FcRn) by Western blot. Serum immunoreactive BSA (iBSA) and mouse IgG1 and IgG2a anti‐OVA antibodies were estimated by enzyme‐linked immunosorbent assay. Sucrase activity was elevated in cortisone‐ and spermidine‐treated compared to control rats. iBSA and anti‐OVA were significantly reduced in cortisone‐treated compared to control rats but were not diminished significantly in the spermidine‐treated animals. A decrease in the neonatal intestinal Fc receptor was apparent in the spermidine‐fed group; cortisone produced a large reduction in FcRn. Spermidine‐fed animals showed morphologic evidence of maturation, with loss of giant vacuoles in the distal intestine; cortisone did not produce significant changes in morphology. Thus, while spermidine, like cortisone, enhanced the appearance of the disaccharidase sucrase, it did not significantly reduce the uptake of BSA or IgG, which is usually observed in mature compared to immature animals. The reason for this dichotomy is not known.

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mentioning

confidence: 99%

Influence of the Polyamine, Spermidine, on Intestinal Maturation and Dietary Antigen Uptake in the Neonatal Rat

Capano,

Bloch,

Schiffrin

et al. 1994

J. pediatr. gastroenterol. nutr.

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Dendritic Cells as Targets for Mucosal Immunization

Rescigno¹,

Citterio²,

Ricciardi‐Castagnoli³

1997

Gram-Positive Bacteria

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The gut as a lymphoepithelial organ: The role of intestinal epithelial cells in mucosal immunity

et al. 1995

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Mucosal surfaces covered by a layer of epithelial cells represent the largest and most critical interface between the organism and its environment. The barrier function of mucosal surfaces is performed by the epithelial layer and immune cells present in the mucosal compartment. As recently found, epithelial cells, apart from their participation in absorptive, digestive and secretory processes perform more than a passive barrier function and are directly involved in immune processes. Besides the well known role of epithelial cells in the transfer of polymeric immunoglobulins produced by lamina propria B lymphocytes to the luminal content of mucosals (secretory Igs), these cells were found to perform various other immunological functions, to interact with other cells of the immune system and to induce an efficient inflammatory response to microbial invasion: enzymic processing of dietary antigens, expression of class I and II MHC antigens, presentation of antigens to lymphocytes, expression of adhesive molecules mediating interaction with intraepithelial lymphocytes and components of extracellular matrix, production of cytokines and probable participation in extrathymic T cell development of intraepithelial lymphocytes. All these functions were suggested to influence substantially the mucosal immune system and its response. Under immunopathological conditions, e.g. during infections and inflammatory bowel and celiac diseases, both epithelial cells and intraepithelial lymphocytes participate substantially in inflammatory reactions. Moreover, enterocytes could become a target of mucosal immune factors. Mucosal immunosurveillance function is of crucial importance in various pathological conditions but especially in the case of the most frequent malignity occurring in the intestinal compartment, i.e. colorectal carcinoma. Proper understanding of the differentiation processes and functions of epithelial cells in interaction with other components of the mucosal immune system is therefore highly desirable.

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Cellular and Molecular Basis for Antigen Transport in the Intestinal Epithelium

Cited by 8 publications

References 135 publications

Influence of the Polyamine, Spermidine, on Intestinal Maturation and Dietary Antigen Uptake in the Neonatal Rat

Influence of the Polyamine, Spermidine, on Intestinal Maturation and Dietary Antigen Uptake in the Neonatal Rat

Dendritic Cells as Targets for Mucosal Immunization

The gut as a lymphoepithelial organ: The role of intestinal epithelial cells in mucosal immunity

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