Cellular and molecular mechanisms involved in branching morphogenesis of the<i>Drosophila</i>tracheal system

Cabernard, Clemens; Neumann, Marc; Affolter, Markus

doi:10.1152/japplphysiol.00435.2004

Cited by 24 publications

(27 citation statements)

References 60 publications

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“…It was also shown that the FGFR signaling pathway is crucial for tracheal cell migration, as MARCM clones mutant for the Drosophila btl/FGFR or for certain downstream effectors of the FGFR signaling pathway remain in the proximal region of the air sac primordium (arrowhead in Figure 2F) and never colonize the migrating tip of the primordium (Cabernard et al 2004). In an attempt to isolate genes involved in tracheal cell migration, mutant lines displaying a migration defect with ,40% of the MARCM clones present at the air sac distal tip were kept for further analysis.…”

Section: Resultsmentioning

confidence: 98%

A Genetic Mosaic Analysis With a Repressible Cell Marker Screen to Identify Genes Involved in Tracheal Cell Migration During Drosophila Air Sac Morphogenesis

et al. 2007

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Branching morphogenesis of the Drosophila tracheal system relies on the fibroblast growth factor receptor (FGFR) signaling pathway. The Drosophila FGF ligand Branchless (Bnl) and the FGFR Breathless (Btl/FGFR) are required for cell migration during the establishment of the interconnected network of tracheal tubes. However, due to an important maternal contribution of members of the FGFR pathway in the oocyte, a thorough genetic dissection of the role of components of the FGFR signaling cascade in tracheal cell migration is impossible in the embryo. To bypass this shortcoming, we studied tracheal cell migration in the dorsal air sac primordium, a structure that forms during late larval development. Using a mosaic analysis with a repressible cell marker (MARCM) clone approach in mosaic animals, combined with an ethyl methanesulfonate (EMS)-mutagenesis screen of the left arm of the second chromosome, we identified novel genes implicated in cell migration. We screened 1123 mutagenized lines and identified 47 lines displaying tracheal cell migration defects in the air sac primordium. Using complementation analyses based on lethality, mutations in 20 of these lines were genetically mapped to specific genomic areas. Three of the mutants were mapped to either the Mhc or the stam complementation groups. Further experiments confirmed that these genes are required for cell migration in the tracheal air sac primordium.

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Section: Resultsmentioning

confidence: 98%

A Genetic Mosaic Analysis With a Repressible Cell Marker Screen to Identify Genes Involved in Tracheal Cell Migration During Drosophila Air Sac Morphogenesis

et al. 2007

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“…The cellular events leading to distinct steps in branching morphogenesis are best understood in the Drosophila tracheal system [4,6,28]. In brief, the onset of fly tracheal development starts at 5 h after egg-laying (AEL) with formation of ten tracheal sacs on each side of the embryo by invagination of the surface ectoderm.…”

Section: Migration Cell Rearrangement and Change Of Cell Shapementioning

confidence: 99%

“…At 7 h AEL, primary branches start to form by invaginating or budding in six different but stereotypical directions. At each bud site, two cells migrate out of the sac, followed by a small number [3][4][5][6][7][8][9][10][11][12][13][14][15][16][17][18][19][20] of cells. As they migrate, cells of the primary branch elongate and intercalate such that their initial side-by-side positioning is rearranged to an end-to-end configuration, with the lumen of resultant tubes enveloped by one single cell.…”

Section: Migration Cell Rearrangement and Change Of Cell Shapementioning

confidence: 99%

“…In essence, tracheal branching creates morphologically distinct tubes, which form apical junctions through a variety of mechanisms [28]. Together, tracheal branching is characterized by three main cellular processes: cell migration, cell rearrangement and change of cell shape [4,6,28].…”

Section: Migration Cell Rearrangement and Change Of Cell Shapementioning

confidence: 99%

“…Many of the molecules and processes involved in fly branching morphogenesis are now known to be highly related to those involved in the development of complex vertebrate organs [4][5][6]. From these studies, it has become clear that a small number of related pathways fulfill a similar role in branching of various systems, whereas molecular differences between distinct branched structures or organs will help explain how each organ becomes unique [2,7,8].…”

Section: Introductionmentioning

confidence: 99%

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Comparative Mechanisms of Branching Morphogenesis in Diverse Systems

Sternlicht

Werb

2006

J Mammary Gland Biol Neoplasia

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Much progress has been made in recent years toward understanding mechanisms controlling branching morphogenesis, a fundamental aspect of development in a variety of invertebrate and vertebrate organs. To gain a deeper understanding of how branching morphogenesis occurs in the mammary gland, we compare and contrast the cellular and molecular events underlying this process in both invertebrate and vertebrate organs. Thus, in this review, we focus on the common themes that have emerged from such comparative analyses and discuss how they are implemented via a battery of signaling pathways to ensure proper branching morphogenesis in diverse systems.

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Drosophila as a model for epithelial tube formation

Maruyama

Andrew

2011

Developmental Dynamics

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Summary Epithelial tubular organs are essential for life in higher organisms and include the pancreas and other secretory organs that function as biological factories for the synthesis and delivery of secreted enzymes, hormones and nutrients essential for tissue homeostasis and viability. The lungs, which are necessary for gas exchange, vocalization and maintaining blood pH, are organized as highly branched tubular epithelia. Tubular organs include arteries, veins and lymphatics, high-speed passageways for delivery and uptake of nutrients, liquids, gases and immune cells. The kidneys and components of the reproductive system are also epithelial tubes. Both the heart and central nervous system of many vertebrates begin as epithelial tubes. Thus, it is not surprising that defects in tube formation and maintenance underlie many human diseases. Accordingly, a thorough understanding how tubes form and are maintained is essential to developing better diagnostics and therapeutics. Among the best-characterized tubular organs are the Drosophila salivary gland and trachea, organs whose relative simplicity have allowed for in depth analysis of gene function, yielding key mechanistic insight into tube initiation, remodeling and maintenance. Here, we review our current understanding of salivary gland and trachea formation – highlighting recent discoveries into how these organs attain their final form and function.

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Cellular and molecular mechanisms involved in branching morphogenesis of theDrosophilatracheal system

Abstract: Cabernard, Clemens, Marc Neumann, and Markus Affolter. Cellular and molecular mechanisms involved in branching morphogenesis of the Drosophila tracheal system.

Cited by 24 publications

References 60 publications

A Genetic Mosaic Analysis With a Repressible Cell Marker Screen to Identify Genes Involved in Tracheal Cell Migration During Drosophila Air Sac Morphogenesis

A Genetic Mosaic Analysis With a Repressible Cell Marker Screen to Identify Genes Involved in Tracheal Cell Migration During Drosophila Air Sac Morphogenesis

Comparative Mechanisms of Branching Morphogenesis in Diverse Systems

Drosophila as a model for epithelial tube formation

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