Cellular and sub-chronic toxicity of hydroxyapatite porous beads loaded with antibiotic in rabbits, indented for chronic osteomyelitis

Vandana, Unnikrishnan; Akhil, V.; Babu, S. Suresh; Francis, Boniface Fernandez; Sabareeswaran, A; Varma, H. K.; Mohanan, P.V.

doi:10.1016/j.ijpharm.2022.121535

Cited by 7 publications

(2 citation statements)

References 33 publications

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“…The chitosan scaffold (SMALD 0%) showed almost similar results as TCP, however the higher concentration of SMALD loading in scaffolds leads to down‐regulation by 2–5% when compared to the control, and this may be attributed to the limited hostile nature of higher concentration of HAP on cell. [ 76–78 ] Moreover, during the observation period, the cells showed no toxicity, and because of the higher surface area, cells continued to divide more than TCP control which confirmed the advantage of the porous structure SMALD scaffolds.…”

Section: Resultsmentioning

confidence: 74%

Modified‐Hydroxyapatite‐Chitosan Hybrid Composite Interfacial Coating on 3D Polymeric Scaffolds for Bone Tissue Engineering

Poddar,

Singh,

Rao

et al. 2023

Macromolecular Bioscience

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Three‐dimensional scaffolds have huge limitations due to their low porosity, mechanical strength, and lack of direct cell‐bioactive drug contact. Whereas a drug like alendronate's ability to stimulate osteogenesis in osteoblasts and bone marrow mesenchymal stem cells has attracted its therapeutic use, it is hard to administer due to its low bioavailability, quick onset, and lack of site‐specificity. The proposed scaffold architecture allows cells to access the bioactive surface at their apex by interacting at the scaffold's interfacial layer. The interface has been coated with alendronate‐modified hydroxyapatite (MALD) enclosed in a chitosan matrix, the inner pore wall structure of 3D polycaprolactone (PCL) scaffolds to mimic the native environment and provide the direct interaction of cell to bioactive layer and mechanical strength will be provided by the skeleton of PCL. The MALD composite's HAP component will govern ALD's behavior, and local calcium ion concentration increases hMSC proliferation and differentiation. MALD showed total release of 86.28±0.22, and particle size of 273.7±108 nm. XPS and SEM investigation of the 3‐D PCL scaffold structure shows inspiring particle deposition with chitosan over the interface and reinforcing. All scaffolds enhanced cell adhesion, proliferation, and osteocyte differentiation for over a week without in vitro cell toxicity with 3.03±0.2 kPa mechanical strength.This article is protected by copyright. All rights reserved

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