Cellular and subcellular localization of EFA6C, a third member of the EFA6 family, in adult mouse Purkinje cells

Matsuya, Shigetsune; Sakagami, Hiroyuki; Tohgo, Akira; Owada, Yuji; Shin, Hye‐Won; Takeshima, Hiroshi; Nakayama, Kazuhisa; Kokubun, S.; Kondo, Hisatake

doi:10.1111/j.1471-4159.2005.03072.x

Cited by 32 publications

(45 citation statements)

References 46 publications

(104 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Northern blot results indicate that EFA6C is mainly expressed in brain, similar to EFA6A, whereas EFA6B shows a widespread tissue distribution (Derrien et al, 2002). EFA6C is detected in spines at the electron microscopic level and enriched in PSD fractions (Matsuya et al, 2005). Our results (Fig.…”

Section: Role Of Efa6a In Spine Development and Maintenancesupporting

confidence: 74%

“…Our results (Fig. 1 B) and previous reports indicate that EFA6C is highly expressed in cerebellar Purkinje cells (Matsuya et al, 2005), in contrast to the preferential expression of EFA6A in forebrain areas (Suzuki et al, 2002) and widespread expression of ARF6 (Suzuki et al, 2001), suggesting that EFA6 family isoforms may differentially interact with ARF6 in brain regions. In addition to EFA6 family proteins, brain expresses other families of ARF GEFs, including ARF GEP 100 (ARF6 specific) (Someya et al, 2001) and ARNO/GRP/cytohesin (ARF1 and ARF6 specific) (Nie et al, 2003).…”

Section: Role Of Efa6a In Spine Development and Maintenancecontrasting

confidence: 54%

See 1 more Smart Citation

ARF6 and EFA6A Regulate the Development and Maintenance of Dendritic Spines

Choi¹,

Ko²,

Lee³

et al. 2006

J. Neurosci.

121

View full text Add to dashboard Cite

The cellular and molecular mechanisms underlying the development and maintenance of dendritic spines are not fully understood. ADP-ribosylation factor 6 (ARF6) is a small GTPase known to regulate actin remodeling and membrane traffic. Here, we report involvement of ARF6 and exchange factor for ARF6 (EFA6A) in the regulation of spine development and maintenance. An active form of ARF6 promotes the formation of dendritic spines at the expense of filopodia. EFA6A promotes spine formation in an ARF6 activation-dependent manner. Knockdown of ARF6 and EFA6A by small interfering RNA decreases spine formation. Live imaging indicates that ARF6 knockdown decreases the conversion of filopodia to spines and the stability of early spines. The spine-promoting effect of ARF6 is partially blocked by Rac1. ARF6 and EFA6A protect mature spines from inactivity-induced destabilization. These results suggest that ARF6 and EFA6A may regulate the conversion of filopodia to spines and the stability of both early and mature spines.

show abstract

Section: Role Of Efa6a In Spine Development and Maintenancesupporting

confidence: 74%

Section: Role Of Efa6a In Spine Development and Maintenancecontrasting

confidence: 54%

ARF6 and EFA6A Regulate the Development and Maintenance of Dendritic Spines

Choi¹,

Ko²,

Lee³

et al. 2006

J. Neurosci.

121

View full text Add to dashboard Cite

show abstract

“…It remains to be elucidated whether a smaller EFA6A polypeptide is an alternatively spliced form or artifact. Subsequent cDNA analyses revealed that the EFA6 family consists of four members (EFA6A, EFA6B, EFA6C, and EFA6D) (Derrien et al 2002;Matsuya et al 2005;Sakagami et al 2006). The overall domain organization is conserved among the EFA6 family in that it comprises a variable N-terminal region, a central catalytic Sec7 domain, a pleckstrin homology (PH) domain and a C-terminal coiled coil motif (Fig.…”

Section: Structurementioning

confidence: 99%

“…A recent phylogenic analysis of Sec 7 domains has proposed to classify 15 mammalian ARF-GEFs into six families (Cox et al 2004): the brefeldin A-inhibited GEF (BIG)/ Sec7 family, Golgi-specifi c brefeldin A-resistance factor (GBF)/GEA family, EFA6 family, cytohesin family, brefeldin A-resistant ARF-GEF (BRAG) family and F-box only protein 8 (FBX8). Among these, ARF-GEFs that can activate ARF6 are four members in the EFA6 family (EFA6A, EFA6B, EFA6C and EFA6D) (Franco et al 1999;Derrien et al 2002;Matsuya et al 2005;Sakagami et al 2006), ARF nucleotide binding site opener (ARNO)/cytohesin-2 (Frank et al 1998) and GRP-1/ARNO-3/cytohesin-3 (Langille et al 1999) in the cytohesin family, and ARF-GEP10 in the BRAG family (Someya et al 2001). We have studied with a special attention to the EFA6 family, because of the following attractive reasons: (1) The EFA6 family is the fi rst ARF-GEF identifi ed to be specifi c for ARF6 (Franco et al 1999).…”

Section: Structurementioning

confidence: 99%

“…The Sec7 domain is a ~200-amino acid region that is critical for the promotion of the guanine nucleotide exchange for ARFs. The ability of the EFA6 family to activate ARF6 was verifi ed by an in vitro GTPγ S binding assay for EFA6A and EFA6B (Franco et al 1999;Derrien et al 2002) and by an ARF pull down assay for EFA6C and EFA6D (Matsuya et al 2005;Sakagami et al 2006). The PH domain is thought to interact with phosphoinositides, thereby recruiting EFA6 to membranes.…”

Section: Structurementioning

confidence: 99%

See 1 more Smart Citation

The EFA6 Family: Guanine Nucleotide Exchange Factors for ADP Ribosylation Factor 6 at Neuronal Synapses

Sakagami

2008

Tohoku J. Exp. Med.

Self Cite

View full text Add to dashboard Cite

ADP ribosylation factor 6 (ARF6) is a member of the ARF family of small GTPases, which mediates a variety of neuronal functions accompanying the structural changes of developing and mature neurons through its regulation of actin cytoskeleton reorganization and membrane traffi c. The activation of ARF6 is strictly regulated by guanine nucleotide exchange factors (GEFs). The EFA6 family is the fi rst member that was identifi ed to be a specifi c GEF for ARF6 and comprises four structurally related polypeptides (EFA6A, EFA6B, EFA6C and EFA6D). Since the cellular and subcelllular localization of GEFs is a critical determinant for the spatiotemporal activation of ARF6 in neurons, I have focused on the EFA6 family from the anatomical point of view to understand the neuronal functions of ARF6. Three members of the EFA6 family (EFA6A, EFA6C and EFA6D) are abundantly expressed in the mouse brain with distinct spatiotemporal patterns. Interestingly, they are enriched particularly in the postsynaptic density fraction, shedding light on the importance of the EFA-ARF6 pathway in neuronal synapses. Here, I will review the recent advances in the expression and functions of the EFA6 family in the nervous system. small GTPase; ADP ribosylation factor; dendrite; postsynaptic density.Tohoku

show abstract

Paraneoplastic encephalitis, psychiatric symptoms, and hypoventilation in ovarian teratoma

Vitaliani

Mason

Ances

et al. 2005

Annals of Neurology

542

347

View full text Add to dashboard Cite

We report four young women who developed acute psychiatric symptoms, seizures, memory deficits, decreased level of consciousness, and central hypoventilation associated with ovarian teratoma (OT) and cerebrospinal fluid (CSF) inflammatory abnormalities. Three patients recovered with treatment of the tumor or immunosuppression and one died of the disorder. Five other OT patients with a similar syndrome and response to treatment have been reported. Our patients' serum or CSF showed immunolabeling of antigens that were expressed at the cytoplasmic membrane of hippocampal neurons and processes and readily accessed by antibodies in live neurons. Immunoprobing of a hippocampal-expression library resulted in the isolation of EFA6A, a protein that interacts with a member of the two-pore-domain potassium channel family and is involved in the regulation of the dendritic development of hippocampal neurons. EFA6A-purified antibodies reproduced the hippocampal immunolabeling of all patients' antibodies and colocalized with them at the plasma membrane. These findings indicate that in a young woman with acute psychiatric symptoms, seizures, and central hypoventilation, a paraneoplastic immune-mediated syndrome should be considered. Recognition of this disorder is important because despite the severity of the symptoms, patients usually recover. The location and function of the isolated antigen suggest that the disorder is directly mediated by antibodies.

show abstract

Cellular and subcellular localization of EFA6C, a third member of the EFA6 family, in adult mouse Purkinje cells

Cited by 32 publications

References 46 publications

ARF6 and EFA6A Regulate the Development and Maintenance of Dendritic Spines

ARF6 and EFA6A Regulate the Development and Maintenance of Dendritic Spines

The EFA6 Family: Guanine Nucleotide Exchange Factors for ADP Ribosylation Factor 6 at Neuronal Synapses

Paraneoplastic encephalitis, psychiatric symptoms, and hypoventilation in ovarian teratoma

Contact Info

Product

Resources

About