2019
DOI: 10.3389/fpls.2019.00971
|View full text |Cite
|
Sign up to set email alerts
|

Cellular and Transcriptional Responses of Resistant and Susceptible Cultivars of Alfalfa to the Root Lesion Nematode, Pratylenchus penetrans

Abstract: The root lesion nematode (RLN), Pratylenchus penetrans , is a migratory species that attacks a broad range of crops, including alfalfa. High levels of infection can reduce alfalfa forage yields and lead to decreased cold tolerance. Currently, there are no commercially certified varieties with RLN resistance. Little information on molecular interactions between alfalfa and P. penetrans , that would shed light on mechanisms of alfalfa resistance to RLN, is available.… Show more

Help me understand this report

Search citation statements

Order By: Relevance

Paper Sections

Select...
2
2
1

Citation Types

3
22
0

Year Published

2020
2020
2024
2024

Publication Types

Select...
7
1

Relationship

1
7

Authors

Journals

citations
Cited by 23 publications
(25 citation statements)
references
References 78 publications
3
22
0
Order By: Relevance
“…These molecular data are in accordance to histological observations which showed that, despite the fact that nematodes are able to penetrate roots, almost no parasitic J2s are able to evolve to further developmental stages in resistant rice plants [ 22 ] or in Mi -expressing tomato plants [ 17 ]. Similar data were obtained for the root lesion nematode Pratylenchus penetrans during infection of alfafa ( Medicago sativa L.) where several transcripts encoding collagen proteins of the nematode cuticle were among the genes highly expressed in the susceptible cultivar but not in the resistant cultivar [ 50 ].…”
Section: Discussionsupporting
confidence: 65%
See 1 more Smart Citation
“…These molecular data are in accordance to histological observations which showed that, despite the fact that nematodes are able to penetrate roots, almost no parasitic J2s are able to evolve to further developmental stages in resistant rice plants [ 22 ] or in Mi -expressing tomato plants [ 17 ]. Similar data were obtained for the root lesion nematode Pratylenchus penetrans during infection of alfafa ( Medicago sativa L.) where several transcripts encoding collagen proteins of the nematode cuticle were among the genes highly expressed in the susceptible cultivar but not in the resistant cultivar [ 50 ].…”
Section: Discussionsupporting
confidence: 65%
“…For instance, Shukla et al [ 17 ] discussed up-regulation of M. incognita genes related to cell-wall degradation and peptidases in the resistant tomato cultivar. In the same way, Vieira et al [ 50 ] stated that nematode genes critical for P. penetrans development and encoding collagen proteins were down-regulated during infection of the resistant alfalfa cultivar. However, knowledge of gene expression in the pre-infective stage would be required to verify if the same pattern occurred for M. incognita , P. penetrans and M. graminicola .…”
Section: Discussionmentioning
confidence: 94%
“…Also, limiting the nematode to cortical tissues may protect the host plants from more severe damage and enable water and nutrients to continue to be transported to the aerial plant tissues. Tannin-like depositions in the nematode-infected root cells, which is usually associated with cytoplasmic disorganization and lack of membrane integrity, has been reported after the infection of different plants by P. penetrans (Townshend et al, 1989;Vieira et al, 2017Vieira et al, , 2019. Also, the thickened cell walls could reflect the deposition of condensed and granular cytoplasm against the walls.…”
Section: Ultrastructural Cellular Changes In Suitable Hosts Infectementioning
confidence: 96%
“…These depositions are often observed as a defence response by plants against pathogen attack and have been suggested as a mechanism by which host plants resist disease progression (Vieira et al, 2017). Accumulation of more tannin-like deposits in cells of resistant cultivars of alfalfa following an infection of P. penetrans has led to the suggestion that the reaction is a resistance mechanism against the root-lesion nematode (Vieira et al, 2019). However, we observed these changes in cells of cultivars of wheat and barley, both of which are known to be susceptible to root-lesion nematodes In conclusion, this study shows that P. curvicauda survives and multiplies in several cereal cultivars and wild germplasms, and ultracellular damage it causes in susceptible wheat and barley cultivars is typical of major root-lesion nematode pests of many important crops.…”
Section: Ultrastructural Cellular Changes In Suitable Hosts Infectementioning
confidence: 99%
“…Infection of the roots by P. penetrans often results in the release of phenolic compounds, oxidation of which has been associated with the browning of the root tissues [ 4 ]. Complex networks of defense genes and secondary metabolites have been identified in alfalfa as part of host responses to P. penetrans infection [ 7 ]. Nevertheless, the molecular mechanisms that RLNs employ to promote virulence remain largely unknown.…”
Section: Introductionmentioning
confidence: 99%