Cellular Antioxidant Properties of Ischnoderma Resinosum Polysaccharide

Abstract

A predominant polysaccharide isolated from Ischnoderma resinosum underwent evaluation for its capacity to scavenge free radicals and its potential antioxidant properties at a cellular-oriented level. This proved that Ischnoderma resinosum polysaccharide (IRP) remarkably curtailed AAPH-induced erythrocyte hemolysis through the inhibition of the generation of ROS (p < 0.05). Rather, it caused the restoration of intracellular antioxidant enzyme (SOD, GSH-Px, and CAT) activities at an acceptable pace and the silencing of intracellular MDA formation, as well as the rescaling of LDH leakage. Furthermore, a model of oxidative stress in HepG2 cells was established by adopting 400 μM of hydrogen peroxide, which suggested that IRP manifests promising antioxidant activity. Notably, after the intervention of IRP in the H2O2-induced HepG2 cells, there was a statistical elevation in cell survivability (p < 0.05). IRP diminished the morphological alterations in the nucleus and decreased the secretion of ROS (p < 0.05), with a dose-dependent abrogation of apoptosis (p < 0.05). Consequently, IRP, which was isolated and purified, was able to scavenge free radicals and possessed favorable antioxidant activity that could dampen the occurrence of oxidative stimulation and effectively alleviate the AAPH-induced erythrocyte hemolysis and H2O2-induced oxidative damage in HepG2 cells. This provides a basis and theoretical reference for the development and utilization of IRP as a natural antioxidant, with emphasis on the exploitation of environmentally friendly and cost-effective antioxidants.

Arzanol, a natural phloroglucinol α‐pyrone, protects HaCaT keratinocytes against H2O2‐induced oxidative stress, counteracting cytotoxicity, reactive oxygen species generation, apoptosis, and mitochondrial depolarization

“…A dose‐dependent decrease in HaCaT cell viability was previously reported in HaCaT cells exposed to H 2 O 2 (100–500 μM) for 12 h, by MTT assay, with a 50% cell mortality at the concentration of 350 μM H 2 O 2 (Zhang et al, 2020), whereas a survival rate of about 60% was reported by Park et al (2020) for HaCaT cells exposed to 500 μM H 2 O 2 for 24 h. In our study, the H 2 O 2 cytotoxic effect was also confirmed by microscopic observation, which allowed to assess the presence of apoptotic features and decreased cell density. Similar morphological alterations were observed in other cell types exposed to H 2 O 2 ‐damage, such as human umbilical vein endothelial cells, neuroblastoma cell line SK‐N‐MC, and HepG2 cells (Bayati et al, 2011; Farshori et al, 2021; Liao et al, 2022). Pre‐treatment with arzanol preserved HaCaT cells from H 2 O 2 cytotoxicity, and significant greater cell viability was observed in arzanol‐treated cells in comparison with those no‐treated exposed to the highest concentrations of oxidants.…”

“…A dose-dependent decrease in HaCaT cell viability was previously reported in HaCaT cells exposed to H 2 O 2 (100-500 μM) for 12 h, by MTT assay, with a 50% cell mortality at the concentration of 350 μM H 2 O 2 (Zhang et al, 2020), whereas a survival rate of about 60% was reported by Park et al (2020) for HaCaT cells exposed to 500 μM H 2 O 2 for 24 h. In our study, the H 2 O 2 cytotoxic effect was also confirmed by microscopic observation, which allowed to assess the presence of apoptotic features and decreased cell density. Similar (Bayati et al, 2011;Farshori et al, 2021;Liao et al, 2022 (Park et al, 2020).…”

Extraction, Purification, and Structural Characterization of Polysaccharides from Sanghuangporus vaninii with Anti-Inflammatory Activity

“…In this study, the antioxidant activity of five SVP polysaccharides was determined in vitro by antioxidant experiments, including the DPPH radial scavenging ability, hydroxyl radial scavenging ability, superoxide anion radical scavenging ability, and reducing ability. These findings further demonstrate that the in vivo biological activity of S. vaninii needs to be further explored in terms of the specific antioxidant indicators SOD and CAT [ 39 ] and the related protein pathway Nrf-2/Keap-1 [ 40 ].…”

The Effect of Preventing Oxidative Stress and Its Mechanisms in the Extract from Sonchus brachyotus DC. Based on the Nrf2-Keap1-ARE Signaling Pathway

“…In our previous work, we have proven that the main functional components of SBE playing a role in the repair of oxidative damage were polysaccharides, alkaloids, and polyphenols by analysis of the primary constituents of SBE [ 18 ]. Liao [ 38 ] showed that Ischnoderma Resinosum polysaccharides had a strong antioxidant activity to protect injured cells, and the protective effect on cells was dose-dependent.…”