Cellular Attributes of <i>Candida albicans</i> Biofilm-Associated in Resistance Against Multidrug and Host Immune System

Kumar, Dushyant; Kumar, Awanish

doi:10.1089/mdr.2022.0347

Cited by 3 publications

(1 citation statement)

References 95 publications

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“…Typically, VVC is caused by the overgrowth of Candida albicans (C. albicans) in the vaginal tract [4] and has been found to recur in up to 8% of women globally [4][5][6]. This interesting microbe can exist in distinct forms and switch phenotype from the usually harmless yeast form to the infectious hyphal form [4,7] and these different morphologies play a significant role in its virulence [6][7][8].…”

Section: Introductionmentioning

confidence: 99%

Selected N-Terpenyl Organoselenium Compounds Possess Antimycotic Activity In Vitro and in a Mouse Model of Vulvovaginal Candidiasis

Liang,

Pacuła-Miszewska,

Obieziurska-Fabisiak

et al. 2023

Molecules

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In the present work, a series of N-terpenyl organoselenium compounds (CHB1-6) were evaluated for antimycotic activity by determining the minimum inhibitory concentration (MIC) for each compound in fluconazole (FLU)-sensitive (S1) and FLU-resistant (S2) strains of Candida albicans (C. albicans). The most active compounds in the MIC screen were CHB4 and CHB6, which were then evaluated for cytotoxicity in human cervical cancer cells (KB-3-1) and found to be selective for fungi. Next, CHB4 and CHB6 were investigated for skin irritation using a reconstructed 3D human epidermis and both compounds were considered safe to the epidermis. Using a mouse model of vulvovaginal candidiasis (VVC), CHB4 and CHB6 both exhibited antimycotic efficacy by reducing yeast colonization of the vaginal tract, alleviating injury to the vaginal mucosa, and decreasing the abundance of myeloperoxidase (MPO) expression in the tissue, indicating a reduced inflammatory response. In conclusion, CHB4 and CHB6 demonstrate antifungal activity in vitro and in the mouse model of VVC and represent two new promising antifungal agents.

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Section: Introductionmentioning

confidence: 99%

Selected N-Terpenyl Organoselenium Compounds Possess Antimycotic Activity In Vitro and in a Mouse Model of Vulvovaginal Candidiasis

Liang,

Pacuła-Miszewska,

Obieziurska-Fabisiak

et al. 2023

Molecules

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Deciphering druggability potential of some proteins of Candida albicans biofilm using subtractive proteomics approach

Kumar,

Kumar

2024

Rend. Fis. Acc. Lincei

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Zingiber officinale Uncovered: Integrating Experimental and Computational Approaches to Antibacterial and Phytochemical Profiling

Sulieman,

Ibrahim,

Alshammari

et al. 2024

Pharmaceuticals

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Background: Zingiber officinale rhizome is widely cultivated in the central region of Sudan (Gezira) and data on the biological properties of this variety grown in Sudan’s climate are scarce. This study aims to comprehensively analyze the antibacterial, antioxidant, phytochemical, and GC-MS properties of Zingiber officinale (ginger rhizome) to explore its potential applications. Methods and Results: The in vitro antibacterial assessment of the aqueous extract of Sudanese ginger revealed moderate activity against Staphylococcus aureus, Salmonella typhi, Pseudomonas aeruginosa, Escherichia coli, and Klebsiella pneumonia, as determined by the disc diffusion method. The inhibition zones ranged from 12.87 ± 0.11 mm to 14.5 ± 0.12 mm at 30 µg/disc. The minimum inhibitory concentration ranged from 6.25 to 25 µg/mL, while the MBC ranged from 25 to 50 µg/mL. The MBC/MIC exhibited a bactericidal effect against all tested bacteria. Phytochemical screening revealed the presence of various chemical constituents, such as saponins, flavonoids, glycosides, alkaloids, steroids, terpenoids, and the absence of tannins in Sudanese ginger rhizome. Furthermore, GC-MS analysis of ginger rhizome identified 22 chemical compounds with retention times ranging from 7.564 to 17.023 min. The identification of 22 chemical compounds through GC-MS analysis further underscores the prospect of harnessing ginger rhizome for the development of novel medications. Computational analyses showed that ginger compounds bind the Protein Data Bank (PDB) codes 1JIJ and 2QZW with high binding affinities, reaching −9.5 kcal/mol. Ginger compounds also established promising molecular interactions with some key residues, satisfactorily explaining the in vitro results and supporting the pharmacokinetic and experimental findings. Conclusions: This study lays the groundwork for future research and pharmaceutical exploration aimed at harnessing the beneficial properties of ginger rhizome for medicinal and therapeutic purposes, particularly its antimicrobial potential.

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Cellular Attributes of Candida albicans Biofilm-Associated in Resistance Against Multidrug and Host Immune System

Cited by 3 publications

References 95 publications

Selected N-Terpenyl Organoselenium Compounds Possess Antimycotic Activity In Vitro and in a Mouse Model of Vulvovaginal Candidiasis

Selected N-Terpenyl Organoselenium Compounds Possess Antimycotic Activity In Vitro and in a Mouse Model of Vulvovaginal Candidiasis

Deciphering druggability potential of some proteins of Candida albicans biofilm using subtractive proteomics approach

Zingiber officinale Uncovered: Integrating Experimental and Computational Approaches to Antibacterial and Phytochemical Profiling

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