Cellular atypia is negatively correlated with immunohistochemical reactivity of CD31 and vWF expression levels in canine hemangiosarcoma

Abstract

Canine hemangiosarcoma (HSA) is one of the most common mesenchymal tumors in dogs. Its high metastatic and growth rates are usually associated with poor prognosis. Neoplastic cells of HSA can show various levels of cellular atypia in the same mass and may consist of various populations at different differentiated stages. Up to present, however, there is no report analyzing their differentiation states by comparing cellular atypia with differentiation-related protein expressions. To evaluate whether cellular atypia can be used as a differentiation marker in HSA, we analyzed correlation between cellular atypia and intensities of CD31 and von Willebrand Factor (vWF) staining in HSA cases. We also compared cellular atypia and expression levels of CD31 and vWF in each growth patterns. Our results show that cellular atypia was negatively correlated to CD31 and vWF expression levels but no significant correlation was found between growth patterns and cellular atypia or CD31 and vWF expression levels. Our study suggests that cellular atypia is useful for identifying differentiation levels in HSA cases. This study also provides useful information to determine differentiation levels of cell populations within HSA based only on morphological analysis, which will aid further HSA research such as identifying undifferentiation markers of endothelial cells or finding undifferentiated cell population in tissue sections.

Spontaneous abdominal hemangiosarcoma in a ferret

“…Conversely, it has been reported that vWF staining is often negative in poorly differentiated hemangiosarcomas 21 and may even be negative in hemangiomas 20 . Although there has been no report on the detailed relationship between the degree of differentiation of tumor cells and CD31 and FVIII-RAg immunoreactivity in vascular tumors of ferrets, our result suggests that vascular tumors in ferrets also express CD31 but that expression of FVIII-RAg may often be weak or absent, as observed in human and canine cases 19 , 20 , 21 , 22 . Although the reason why FVIII-RAg staining is sometimes negative in vascular tumors in humans and dogs is still not well understood, many negative or weakly positive cases are reported in poorly differentiated tumors with solid proliferation 19 , 20 , 21 , 22 .…”

“…Cellular atypia is reported to be negatively correlated with CD31 and vWF expression in canine hemangiosarcoma 19 . Although there have not been detailed CD31 and vWF expression comparisons, Maharani et al .…”

“…Although there have not been detailed CD31 and vWF expression comparisons, Maharani et al . mentioned that some tumor cells with high cellular atypia scores were positive for CD31 but negative for vWF 19 . Additionally, tumor cells with mild cellular atypia were more likely to be negative for vWF than for CD31 19 .…”

Notch2 signal is required for the maintenance of canine hemangiosarcoma cancer stem cell-like cells

“…These cases were derived from the spleen, liver, kidney and thoracic cavity (Table 2 ). Tissue samples were processed routinely as described previously [ 47 ]. The slides were immersed in 10 mM sodium citrate buffer (pH 6.0), boiled for 15 mins in a microwave for antigen retrieval and then cooled down to RT.…”

<p>Intracerebral Hemorrhage Induced Brain Injury Is Mediated by the Interleukin-12 Receptor in Rats</p>

“…CD31, also known as Platelet endothelial cell adhesion molecule-1 (PECAM-1/CD31), is a biomarker of the endothelial cells and angiogenesis. 30 It is known that neovascularization restores blood and oxygen supply to the tissues to attenuate the hypoxic-ischemic induced injury and promote repair of the tissues, which reverses the apoptosis events in the neurons, glial and endothelial cells. 9 From the current study results, we also observed that blocking the IL-12 receptors promoted angiogenesis in the hematoma areas of rats with ICH, which can be used to attenuate the ICH associated brain damage in rats.…”