Cellular autofluorescence is magnetic field sensitive

Ikeya, Noboru; Woodward, Jonathan

doi:10.1073/pnas.2018043118

Cited by 63 publications

(122 citation statements)

References 39 publications

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“…However, a recent study reports that FAD RPs are MF-sensitive at physiological pH, albeit to a lesser extent than at acidic pH [55]. Moreover, a new study has revealed that flavin autofluorescence in HeLa cells is sensitive to MF [56]. Although this is not a functional response, their observations are consistent with an underlying RPM and represent an exciting example of an MF effect on a chemical reaction in living cells.…”

Section: Discussionmentioning

confidence: 91%

Exploiting the Fruitfly, Drosophila melanogaster, to Identify the Molecular Basis of Cryptochrome-Dependent Magnetosensitivity

et al. 2021

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The flavoprotein CRYPTOCHROME (CRY) is now generally believed to be a magnetosensor, providing geomagnetic information via a quantum effect on a light-initiated radical pair reaction. Whilst there is considerable physical and behavioural data to support this view, the precise molecular basis of animal magnetosensitivity remains frustratingly unknown. A key reason for this is the difficulty in combining molecular and behavioural biological experiments with the sciences of magnetics and spin chemistry. In this review, we highlight work that has utilised the fruit fly, Drosophila melanogaster, which provides a highly tractable genetic model system that offers many advantages for the study of magnetosensitivity. Using this “living test-tube”, significant progress has been made in elucidating the molecular basis of CRY-dependent magnetosensitivity.

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Section: Discussionmentioning

confidence: 91%

Exploiting the Fruitfly, Drosophila melanogaster, to Identify the Molecular Basis of Cryptochrome-Dependent Magnetosensitivity

et al. 2021

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“…Flavins generally provide a rich source of MFEs due to their ability to form RPs in photo-induced electron transfer reactions with various electron donors [252], both from the S and the T excited states [253]. Recently, the endogenous autofluorescence of HeLa cells has been found to respond to external MFs due to mitochondrial, triplet-born, flavin-containing RPs [254].…”

Section: Is Spin-correlation Common In Biology?mentioning

confidence: 99%

Quantum Biology: An Update and Perspective

et al. 2021

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Understanding the rules of life is one of the most important scientific endeavours and has revolutionised both biology and biotechnology. Remarkable advances in observation techniques allow us to investigate a broad range of complex and dynamic biological processes in which living systems could exploit quantum behaviour to enhance and regulate biological functions. Recent evidence suggests that these non-trivial quantum mechanical effects may play a crucial role in maintaining the non-equilibrium state of biomolecular systems. Quantum biology is the study of such quantum aspects of living systems. In this review, we summarise the latest progress in quantum biology, including the areas of enzyme-catalysed reactions, photosynthesis, spin-dependent reactions, DNA, fluorescent proteins, and ion channels. Many of these results are expected to be fundamental building blocks towards understanding the rules of life.

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“…The fact that Luc-CTW(536)F is sufficient to support magnetosensitivity implies that a different, non-CRY, RP is involved. In this regard, it is notable that free FAD is capable of generating a magnetically sensitive RP via intramolecular electron transfer 7,34 . To explore this, we supplemented additional FAD to aCC via the internal patch saline.…”

Section: Free Fad Supports Magnetoreceptionmentioning

confidence: 99%

Essential elements of radical pair magnetosensitivity inDrosophila

Bradlaugh

Fedele²,

Munro

et al. 2021

Preprint

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SummaryMany animals use the Earth’s magnetic field (geoMF) for navigation1. The favored mechanism for magnetosensitivity involves a blue-light (BL) activated electron transfer reaction between flavin adenine dinucleotide (FAD) and a chain of tryptophan (Trp) residues within the photoreceptor protein, CRYPTOCHROME (CRY). The spin-state of the resultant radical pair (RP) and hence the concentration of CRY in its active state is influenced by the geoMF2. The canonical CRY-centric radical pair mechanism (RPM) does not, however, explain many physiological and behavioural observations2–8. Here, using electrophysiology and behavioural analyses, we assay magnetic field (MF) responses at single neuron and organismal level. We show that the 52 C-terminal (CT) amino acids of CRY, which are missing the FAD binding domain and the Trp chain, are sufficient to facilitate magnetoreception. We also show that increasing intracellular FAD potentiates both BL-induced and MF-dependent effects on the activity mediated by the CT. Additionally, high levels of FAD alone are sufficient to cause BL neuronal sensitivity and, remarkably, potentiation of this response in the co-presence of a MF. These unexpected results reveal the essential components of a primary magnetoreceptor in flies, providing strong evidence that non-canonical (i.e., non-CRY-dependent) RPs can elicit MF responses in cells.

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Cellular autofluorescence is magnetic field sensitive

Cited by 63 publications

References 39 publications

Exploiting the Fruitfly, Drosophila melanogaster, to Identify the Molecular Basis of Cryptochrome-Dependent Magnetosensitivity

Exploiting the Fruitfly, Drosophila melanogaster, to Identify the Molecular Basis of Cryptochrome-Dependent Magnetosensitivity

Quantum Biology: An Update and Perspective

Essential elements of radical pair magnetosensitivity inDrosophila

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