Cellular Characterization of Multidrug Resistance P-glycoprotein, Alpha Fetoprotein, and Neovascular Endothelium-Associated Antigens in Canine Hepatocellular Carcinoma and Cirrhotic Liver

Tashbaeva, R. E.; Hwang, Du Na; Song, Guang; Choi, Nami; Lee, J.-H.; Lyoo, Young S.; Lee, S.-J.; Jung, Dong In; Kim, H.-Y.; Sur, Jung-Hyang

doi:10.1354/vp.44-5-600

Cited by 27 publications

(14 citation statements)

References 26 publications

(53 reference statements)

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“…IHC was performed as described previously with a few modifications [28]. Briefly, slides were deparaffinized in xylene then hydrated in graded ethanol.…”

Section: Methodsmentioning

confidence: 99%

Canine classical seminoma: a specific malignant type with human classifications is highly correlated with tumor angiogenesis

Kim

Yhee

et al. 2010

BMC Cancer

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BackgroundHuman seminoma is classified as classical seminoma (SE) and spermatocytic seminoma (SS). Human SE is known to be more malignant and metastasizing more frequently than SS. Tumor angiogenesis is highly related with tumor progression and metastasis, with microvessel density (MVD) being an important parameter of metastatic potential. Canine seminoma is not yet well-established as SE or SS type including correlation with angiogenesis. We classified canine SE and SS, and then compared them to tumor associated vessels.MethodsTwenty-three cases of canine seminomas (2 intratubular, 9 diffuse, and 12 intratubular/diffuse seminomas showing both intratubular and diffuse patterns) were classified as SE or SS by immunohistochemistry (IHC) using monoclonal antibody against PLAP and by PAS stain. The histopathological data were then compared to see if there was a correlation with SE or SS. Angiogenesis of seminomas were evaluated by immunohistochemical assay using polyclonal antibody against Von Willebrand factor (vWF) and by calculating the means of MVD, vessels area and perimeters using computerized image analysis. Statistical Package for Social Sciences (SPSS) program was used for various statistical analyses.ResultsThe numbers of PLAP+/PAS+ canine SEs were 8/23 (34.8%) and PLAP-/PAS- SSs were 15/23 (61.2%). All SE cases (8/8, 100%) were intratubular/diffuse types. SS types included 2 intratubular (2/15, 13.3%), 9 diffuse (9/15, 60%), and 4 intratubular/diffuse (4/15, 26.7%) types. MVD and vascular parameters in SEs were significantly higher than in SSs, showing the highest value in the intratubular/diffuse type. Seminomas observed with neoplastic cells invasion of vessels presented higher perimeter and area values than seminomas without conformed neoplastic cells invasion.ConclusionIn this study, we demonstrated a positive relationship between canine SE and tumor angiogenesis. Furthermore, we also showed that a tumor cells invasion of vessels were a correlated vascular parameter. Although metastasis of canine seminomas has rarely been reported, our results support that canine SE could have high metastatic potential similar to the human counterpart. Further studies are required to clarify the relationship between canine SE and clinical data with metastatic factors.

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“…IHC was performed as described previously with a few modifications [28]. Briefly, slides were deparaffinized in xylene then hydrated in graded ethanol.…”

Section: Methodsmentioning

confidence: 99%

Canine classical seminoma: a specific malignant type with human classifications is highly correlated with tumor angiogenesis

Kim

Yhee

et al. 2010

BMC Cancer

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“…[5] [7] From various studies it was found that P-gp was more highly expressed in HCC (hepatocellular carcinoma) than in cirrhotic liver, which is consistent with the known resistance of HCC to chemotherapy. [5] RT-PCR can be one of the most sensitive, efficient, fast, and reproducible methods of measuring gene expression. RT-PCR can be used to quantify mRNA levels from much smaller samples.…”

Section: Discussionmentioning

confidence: 78%

“…P-gp is normally expressed in tissues with excretory function, including the jejunum, kidney, liver, and adrenal gland. [5] Over-expression of MDR1 will result in increased drug efflux activity of P-gp leading to multi-drug resistance (MDR).…”

Section: Introductionmentioning

confidence: 99%

P-glycoprotein expression as a predictor of response to neoadjuvant chemotherapy in breast cancer

Vishnukumar¹,

Umamaheswaran²,

Anichavezhi³

et al. 2013

Indian J Cancer

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“…The anti-CD34 is considered the best immuno-histochemical endothelial marker for tricolor staining (Cui and Hano, 1996). Other detailed references can refer to Pusztaszeri et al (2006) and Tashbaeva et al (2007).…”

Section: Materials and System Overview Microscopic Image Samples Of Omentioning

confidence: 99%

Color‐based tumor tissue segmentation for the automated estimation of oral cancer parameters

Sun

Wang

Chang

et al. 2009

Microscopy Res & Technique

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This article presents an automatic color-based feature extraction system for parameter estimation of oral cancer from optical microscopic images. The system first reduces image-to-image variations by means of color normalization. We then construct a database which consists of typical cancer images. The color parameters extracted from this database are then used in automated online sampling from oral cancer images. Principal component analysis is subsequently used to divide the color features into four tissue types. Each pixel in the cancer image is then classified into the corresponding tissue types based on the Mahalanobis distance. The aforementioned procedures are all fully automated; in particular, the automated sampling step greatly reduces the need for intensive labor in manual sampling and training. Experiments reveal high levels of consistency among the results achieved using the manual, semiautomatic, and fully automatic methods. Parameter comparisons between the four cancer stages are conducted, and only the mean parameters between early and late cancer stages are statistically different. In summary, the proposed system provides a useful and convenient tool for automatic segmentation and evaluation for stained biopsy samples of oral cancer. This tool can also be modified and applied to other tissue images with similar staining conditions.

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Cellular Characterization of Multidrug Resistance P-glycoprotein, Alpha Fetoprotein, and Neovascular Endothelium-Associated Antigens in Canine Hepatocellular Carcinoma and Cirrhotic Liver

Cited by 27 publications

References 26 publications

Canine classical seminoma: a specific malignant type with human classifications is highly correlated with tumor angiogenesis

Canine classical seminoma: a specific malignant type with human classifications is highly correlated with tumor angiogenesis

P-glycoprotein expression as a predictor of response to neoadjuvant chemotherapy in breast cancer

Color‐based tumor tissue segmentation for the automated estimation of oral cancer parameters

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