Cellular Choline and Glycine Betaine Pools Impact Osmoprotection and Phospholipase C Production in Pseudomonas aeruginosa

Fitzsimmons, Liam F.; Hampel, Ken J.; Wargo, Matthew J.

doi:10.1128/jb.00596-12

Cited by 46 publications

(49 citation statements)

References 62 publications

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Section: Regulation Of Aerobic Choline and Gb Catabolism In Pseudomonadsmentioning

confidence: 99%

“…Our recent data in P. aeruginosa show that cells do maintain a GB pool during growth on choline as a sole carbon source, even under conditions of high salinity (65). The presence of this pool is not absolute, as it was drained during the stationary phase, likely to augment growth or maintenance, and may be accompanied by synthesis of alternative osmoprotectants (65). Depletion of the cytoplasmic GB pool was experimentally achieved by overexpression of the GB demethylase (GbcAB), supporting a model where enzyme production, and not enzyme regulation, is likely the step targeted by regulation (65).…”

Section: Regulation Of Aerobic Choline and Gb Catabolism In Pseudomonadsmentioning

confidence: 99%

“…In P. aeruginosa, both choline and GB can be used as sole carbon sources in the presence of substantial osmostress (750 mM NaCl) (65). This leads to the following question: how does P. aeruginosa simultaneously catabolize its osmoprotectant and still survive the osmostress?…”

Section: Regulation Of Aerobic Choline and Gb Catabolism In Pseudomonadsmentioning

confidence: 99%

“…The presence of this pool is not absolute, as it was drained during the stationary phase, likely to augment growth or maintenance, and may be accompanied by synthesis of alternative osmoprotectants (65). Depletion of the cytoplasmic GB pool was experimentally achieved by overexpression of the GB demethylase (GbcAB), supporting a model where enzyme production, and not enzyme regulation, is likely the step targeted by regulation (65). However, the identity of the regulatory step(s) that monitors and maintains GB levels is currently unknown.…”

Section: Regulation Of Aerobic Choline and Gb Catabolism In Pseudomonadsmentioning

confidence: 99%

“…The AraC family members canonically regulate genes involved in metabolism (e.g., AraC and XylS for regulation of arabinose and xylene/benzoate metabolism, respectively [59,60]) and/or virulence (61) (e.g., ExsA regulation of type III secretion in P. aeruginosa [62] and ToxT regulation of cholera toxin and the toxincoregulated pilus in Vibrio cholerae [63]). Evidence suggests that GbdR senses GB and dimethylglycine levels in the cell and induces transcription of genes involved in virulence, GB transport, GB catabolism, and detoxification of the catabolic byproducts, hydrogen peroxide and formaldehyde (48,56,(64)(65)(66)(67)(68) (Fig. 2).…”

Section: Choline and Gb Import Versus De Novo Synthesismentioning

confidence: 99%

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Homeostasis and Catabolism of Choline and Glycine Betaine: Lessons from Pseudomonas aeruginosa

Wargo

2013

Appl Environ Microbiol

155

121

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Most sequenced bacteria possess mechanisms to import choline and glycine betaine (GB) into the cytoplasm. The primary role of choline in bacteria appears to be as the precursor to GB, and GB is thought to primarily act as a potent osmoprotectant. Choline and GB may play accessory roles in shaping microbial communities, based on their limited availability and ability to enhance survival under stress conditions. Choline and GB enrichment near eukaryotes suggests a role in the chemical relationships between these two kingdoms, and some of these interactions have been experimentally demonstrated. While many bacteria can convert choline to GB for osmoprotection, a variety of soil-and water-dwelling bacteria have catabolic pathways for the multistep conversion of choline, via GB, to glycine and can thereby use choline and GB as sole sources of carbon and nitrogen. In these choline catabolizers, the GB intermediate represents a metabolic decision point to determine whether GB is catabolized or stored as an osmo-and stress protectant. This minireview focuses on this decision point in Pseudomonas aeruginosa, which aerobically catabolizes choline and can use GB as an osmoprotectant and a nutrient source. P. aeruginosa is an experimentally tractable and ecologically relevant model to study the regulatory pathways controlling choline and GB homeostasis in choline-catabolizing bacteria. The study of P. aeruginosa associations with eukaryotes and other bacteria also makes this a powerful model to study the impact of choline and GB, and their associated regulatory and catabolic pathways, on host-microbe and microbe-microbe relationships.

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Section: Regulation Of Aerobic Choline and Gb Catabolism In Pseudomonadsmentioning

confidence: 99%

Section: Regulation Of Aerobic Choline and Gb Catabolism In Pseudomonadsmentioning

confidence: 99%

Section: Regulation Of Aerobic Choline and Gb Catabolism In Pseudomonadsmentioning

confidence: 99%

Section: Regulation Of Aerobic Choline and Gb Catabolism In Pseudomonadsmentioning

confidence: 99%

Section: Choline and Gb Import Versus De Novo Synthesismentioning

confidence: 99%

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Homeostasis and Catabolism of Choline and Glycine Betaine: Lessons from Pseudomonas aeruginosa

Wargo

2013

Appl Environ Microbiol

155

121

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Metabolite composition of sinking particles differs from surface suspended particles across a latitudinal transect in the South Atlantic

Johnson

Longnecker

Soule

et al. 2019

Limnology & Oceanography

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Marine sinking particles transport carbon from the surface and bury it in deep‐sea sediments, where it can be sequestered on geologic time scales. The combination of the surface ocean food web that produces these particles and the particle‐associated microbial community that degrades them creates a complex set of variables that control organic matter cycling. We use targeted metabolomics to characterize a suite of small biomolecules, or metabolites, in sinking particles and compare their metabolite composition to that of the suspended particles in the euphotic zone from which they are likely derived. These samples were collected in the South Atlantic subtropical gyre, as well as in the equatorial Atlantic region and the Amazon River plume. The composition of targeted metabolites in the sinking particles was relatively similar throughout the transect, despite the distinct oceanic regions in which they were generated. Metabolites possibly derived from the degradation of nucleic acids and lipids, such as xanthine and glycine betaine, were an increased mole fraction of the targeted metabolites in the sinking particles relative to surface suspended particles, while algal‐derived metabolites like the osmolyte dimethylsulfoniopropionate were a smaller fraction of the observed metabolites on the sinking particles. These compositional changes are shaped both by the removal of metabolites associated with detritus delivered from the surface ocean and by production of metabolites by the sinking particle‐associated microbial communities. Furthermore, they provide a basis for examining the types and quantities of metabolites that may be delivered to the deep sea by sinking particles.

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Signal pattern plot: a simple tool for time-dependent metabolomics studies by 1H NMR spectroscopy

Bachmann

Jilani

Ibrahim³

et al. 2019

Anal Bioanal Chem

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Cellular Choline and Glycine Betaine Pools Impact Osmoprotection and Phospholipase C Production in Pseudomonas aeruginosa

Cited by 46 publications

References 62 publications

Homeostasis and Catabolism of Choline and Glycine Betaine: Lessons from Pseudomonas aeruginosa

Homeostasis and Catabolism of Choline and Glycine Betaine: Lessons from Pseudomonas aeruginosa

Metabolite composition of sinking particles differs from surface suspended particles across a latitudinal transect in the South Atlantic

Signal pattern plot: a simple tool for time-dependent metabolomics studies by 1H NMR spectroscopy

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