Cellular electrophoretic mobility data: A first approach to a database

Abstract

Cellular electrophoretic mobility values of 288 types of eucaryotic cells were collected from literature published worldwide by a series of authors during the past forty years and arranged in a list. This list contains well-known recent electrophoretic results and also data that cannot be found anymore with modern literature retrieval systems. It will be a valuable help for scientists trying to purify cell populations. In addition, it confirms the observation that most eucaryotic cells have very similar electrophoretic mobilities, ranging from 40% above to 50% below the electrophoretic mobility of human erythrocytes, and thus reinforces the suggestion that electrophoretic mobilities of eucaryotic cells are subjected to strong biological controls.

The Therapeutic Potential of Fungal Ribotoxins

“…The current working hypothesis sustains that a higher content of negatively charged phospholipids in transformed cell membranes [53,54,[57][58][59][60] would explain the favored toxicity of these proteins against tumors.…”

Electrophoretic Mobility of Mouse Leukocytes Evaluated Using Microcapillary Electrophoresis Chips

“…Cell electrophoresis is practically the only method available for estimating the cell surface charge density [3][4][5][6]. Cell electrophoretic mobility (EPM), which can be measured by a cell electrophoresis experiment, is a characteristic physical property that reflects the electrical and mechanical properties of the cell surface.…”

“…Since 1960, a few studies concerning the correlation between cell EPM and biological condition have been studied. However, the importance of cell EPM has been underestimated for a long time mostly because of the technical difficulties associated with its reliable measurement [3,6]. When cell electrophoresis experiments were performed using traditional instruments, electroosmosis generated the circulating flow inside a closed chamber called an electrophoretic tank.…”

Cell separation with horizontal cell electrophoresis under near-isopycnic conditions on a “density cushion”

“…The analysis of the electrochemical features of cell surfaces is applicable to those changes which occur during the cell cycle and cell differentiation, and due to a variety of pathological states, including neoplastic transformation [1][2][3][4]. Cells from different species are similarly characterized by different electrophoretic mobilities [5,6]. Differences in the physical properties of cell surfaces may also be used to separate cell subpopulations from cell mixtures via electrophoresis, especially when specific cell surface antibodies are unavailable and separation using flow cytometry is impossible.…”

“…Preparative cell electrophoresis was achieved with the free-flow curtain apparatus [10], which is now commercially available [11]. Our earlier paper described a horizontal electrophoresis system which effectively separated human and chicken red blood cells (RBC), which differ by about 30% in their electrophoretic mobilities [5,12]. The separation was carried out under near-isopycnic conditions, when cell sedimentation is negligible, within an anticonvective porous matrix of polyurethane sponge with a pore size over 300 μm.…”