Cellular electrophysiologic and mechanical evidence of superior vascular protection in pulmonary microcirculation by Perfadex compared with Celsior

Abstract

This in vitro study simulating clinical conditions demonstrates that Perfadex preserves endothelium-dependent smooth muscle relaxation and hyperpolarization better than Celsior solution in regard to the electrophysiologic and mechanical properties observed at cellular and vascular levels. This study provides a new method at the level of basic science to evaluate the solutions for heart/lung preservation.

Review of donor and recipient surgical procedures in lung transplantation

“…The topic of optimal preservation solution has been evaluated extensively. On a cellular level, high potassium preservation solutions resulted in decreased membrane potential hyperpolarization, more depolarization of the resting membrane potential, and decreased hyperpolarizationassociated relaxation of the pulmonary arterioles as compared to extracellular based low-potassium solution (Perfadex) (8). From a clinical standpoint, intracellular high-potassium solutions resulted in a higher incidence and severity of reperfusion injury as compared to the extracellular based low-potassium Perfadex solution (9).…”

The assessment of the quality of the graft in an animal model for lung transplantation using the metabolomics 1H high‐resolution magic angle spinning NMR spectroscopy

“…Therefore, we propose in our work to investigate the possibility of implementing a selective in situ perfusion of the small pulmonary circulation by a preservation solution while maintaining pulmonary ventilation. Perfadex V R solution is widely used in the current practice, as it is reported to contribute to reduce primary graft dysfunction after lung transplantation (3)(4)(5). The quality of the graft was assessed using the metabolomics 1 H high-resolution magic angle spinning (HRMAS) NMR spectroscopy of the lung biopsy samples.…”