Cellular expression of a monocarboxylate transporter (MCT1) in the mammary gland and sebaceous gland of mice

Takebe, Kumiko; Nio‐Kobayashi, Junko; Takahashi-Iwanaga, Hiromi; Yajima, Takaji; Iwanaga, Toshihiko

doi:10.1007/s00418-008-0543-3

Cited by 21 publications

(26 citation statements)

References 34 publications

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“…Acetate, ketone bodies, and other monocarboxylates can serve as materials for lipogenesis. MCT1 in adults under normal conditions may be useful for the uptake of these materials for lipogenesis (125), as supported by cases of sebaceous glands and mammary glands (143) (Fig. 5e, f).…”

Section: Lipogenetic Tissuesmentioning

confidence: 84%

“…The corneal endothelium does contain at least some MCT1 immunoreactivity in the mouse. The harderian gland, especially developed in rodents, is a lipid-secreting lacrimal gland behind the ocular bulb and intensely expresses MCT1 along the basolateral plasma membrane (143). This may reflect a great demand for monocarboxylates as materials for the production of lipids.…”

Section: The Retina and Other Ocular Tissuesmentioning

confidence: 99%

“…It is generally considered that MCT1 in adipose cells is related to lactate production and whole body lactate metabolism. On the other hand, MCTs in some lipogenetic tissues are involved in the production of steroids and medium/long-chain fatty acids (143). Acetate, ketone bodies, and other monocarboxylates can serve as materials for lipogenesis.…”

Section: Lipogenetic Tissuesmentioning

confidence: 99%

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Cellular distributions of monocarboxylate transporters: a review

Iwanaga

Kishimoto

2015

Biomed. Res.

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Lactate and ketone bodies play important roles as alternative energy substrates, especially in conditions with a decreased utility of glucose. Short-chain fatty acids (acetate, propionate, and butyrate), produced by bacterial fermentation, supply most of the energy substrates in ruminants such as the cow and sheep. These monocarboxylates are transfered through the plasma membrane by proton-coupled monocarboxylate transporters (MCTs) and sodium-coupled MCTs (SMCTs). To reveal the metabolism and functional significance of monocarboxylates, the cellular localization of MCTs and SMCTs together with the expressed intensities holds great importance. This paper reviews the immunohistochemical localization of SMCTs and major MCT subtypes throughout the mammalian body. MCTs and SMCTs display a selective membrane-bound localization with porality. In contrast to the limited expression of SMCTs in the intestine and kidney, MCTs display a broader distribution pattern than GLUTs. The brain, kidney, placenta, and male genital tract express multiple subtypes of the MCT family. Determination of the cellular localization of MCTs is most controversial in the brain, possibly due to regional differences and the transcriptional modification of MCT proteins. Information on the localization of MCTs and SMCTs aids in understanding the nutrient absorption and metabolism throughout the mammalian body. In some cases, the body may use monocarboxylates as signal molecules, like hormones.

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Section: Lipogenetic Tissuesmentioning

confidence: 84%

Section: The Retina and Other Ocular Tissuesmentioning

confidence: 99%

Section: Lipogenetic Tissuesmentioning

confidence: 99%

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Cellular distributions of monocarboxylate transporters: a review

Iwanaga

Kishimoto

2015

Biomed. Res.

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“…Two non-overlapping antisense oligonucleotide DNA probes (45 mer in length) were designed for each mRNA of mouse MCT1 and GLUT1, as described in our previous study (15,35). The probes were labeled with 33 P-dATP using terminal deoxynucleotidyl transferase (Invitrogen, Carlsbad, CA).…”

Section: Resultsmentioning

confidence: 99%

Histochemical demonstration of a monocarboxylate transporter in the mouse perineurium with special reference to GLUT1

et al. 2008

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Peripheral nerves express GLUT1 in both endoneurial blood vessels and the perineurium and utilize glucose as a major energy substrate, as does the brain. However, under conditions of a reduced utilization of glucose, the brain is dependent upon monocarboxylates such as ketone bodies and lactate, being accompanied by an elevated expression of a monocarboxylate transporter (MCT1) in the blood-brain barrier. The present immunohistochemical study aimed to examine the expression of MCT1 in the peripheral nerves of mice. MCT1 immunoreactivity was found in the perineurial sheath and colocalized with GLUT1, while the endoneurial blood vessels expressed GLUT1 only. An intense expression of MCT1 in the perineurium was confirmed by Western blot and in situ hybridization analyses. Ultrastructurally, the MCT1 and GLUT1 immunoreactivities in the thick perineurium showed an intensity gradient decreasing towards the innermost layer. In neonates, the MCT1 immunoreactivity in the perineurium was intense, while the GLUT1 immunoreactivity was faint or absent. These findings suggest that peripheral nerves depend on monocarboxylates as a major energy source and that MCT1 in the perineurium is responsible for the supply of monocarboxylates to nerve fibers and Schwann cells.

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“…were designed for each mRNA of mouse MCT1-MCT12, GLUT1-GLUT5, and CD147, as described in our previous studies [21][22][23]. CD147 is an important chaperone protein of MCT1 and MCT4 [18].…”

Section: In Situ Hybridizationmentioning

confidence: 99%

Cellular Expression of the Monocarboxylate Transporter (MCT) Family in the Placenta of Mice

et al. 2010

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Lactate plays an important role as an alternative energy substrate, especially in conditions with a decreased utility of glucose. Proton-coupled monocarboxylate transporters (MCTs) are essential for the transport of lactate, ketone bodies, and other monocarboxylates through the plasma membrane and may contribute to the net transport of lactate through the placental barrier. The present study examined the expression profile and subcellular localization of MCTs in the mouse placenta. An in situ hybridization survey of all MCT subtypes detected intense mRNA expressions of MCT1, MCT4, and MCT9 as well as GLUT1 in the placenta from gestational day 11.5.The expression of MCT mRNAs decreased in the intensity at the end of gestation in contrast to a consistently intense expression of GLUT1 mRNA.Immunohistochemically, MCT1 and MCT4 showed a polarized localization on the maternal side and fetal side of the two cell-layered syncytiotrophoblast, respectively.The membrane-oriented localization of MCTs was supported by the coexistence of CD 147 which recruits MCT to the plasma membrane. However, the subcellular arrangement of MCT1 and MCT4 along the trophoblastic cell membrane was completely opposite of that in the human placenta. Although we cannot exactly explain the reversed localization of MCTs between human and murine placentas, it may be related to differences between humans and mice in the origin of lactate and its utilization by fetuses.3

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Cellular expression of a monocarboxylate transporter (MCT1) in the mammary gland and sebaceous gland of mice

Cited by 21 publications

References 34 publications

<b>Cellular distributions of monocarboxylate transporters: a r</b><b>eview </b>

<b>Cellular distributions of monocarboxylate transporters: a r</b><b>eview </b>

Histochemical demonstration of a monocarboxylate transporter in the mouse perineurium with special reference to GLUT1

Cellular Expression of the Monocarboxylate Transporter (MCT) Family in the Placenta of Mice

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