the aim of the study was to determine the cellular lipids fatty acid composition for identification of the Bacillus subtilis onu551 strain bacteria, which is a phenol destructor. Fatty acids analysis of B. subtilis onu551 strain was performed using an automatic system for microorganisms' identification Midi sherlock (Midi, usa) based on gas chromatograph agilent 7890. chromatograms analysis showed that the fatty acid spectrum of the strain B. subtilis onu551 consisted predominately of branched structural isomers of satu