Cellular Fatty Acid Composition of Lautropia mirabilis

Abstract

Ten strains of Lautropia mirabilis (ATCC 51599T and nine phenotypically similar clinical isolates) were examined for cellular fatty acid (CFA) composition to evaluate their chemical relatedness to known bacterial species and groups. The CFAs were liberated from whole cells by base hydrolysis, methylated, and analyzed by gas-liquid chromatography. CFA profiles were generated by using a commerical software package (MIDI, Newark, Del.). All strains tested had an identical CFA profile characterized by major amounts of 16:17c (41%) and 16:0 (44%); smaller amounts (1 to 4%) of 3-OH-10:0, 12:0, 14:0, 15:0, and 18:1 7c; trace amounts (<1%) of 10:0, 18:2 and 18:0; and no cyclopropane acids. This profile was similar to the CFA profiles of Acidovorax delafieldii, Comamonas terrigena, and strains of an unclassified Centers for Disease Control group designated weak oxidizer group 1. CFA analysis, when supplemented by phenotypic characterization, is useful for the identification of L. mirabilis isolates.

Evaluating the use of fatty acid profiles to identify deep-sea Vibrio isolates

“…GC-FID analysis of chemical components of bacterial cells has provided useful information for rapid detection and identification of bacteria in clinical and diagnostic bacteriology laboratories and currently has increased significance for both food safety and security (Daneshvar, Douglas, & Weyant, 2001;Whittaker, 2009;Whittaker, Day, Curtis, & Fry, 2007;Whittaker et al, 2005).…”

Comparison of Yersinia pestis to other closely related Yersinia species using fatty acid profiles

“…GC-FID analysis of chemical components of bacterial cells has provided useful information for rapid detection and identification of bacteria in clinical and diagnostic bacteriology laboratories and currently has increased significance for both food safety and security (Daneshvar, Douglas, & Weyant, 2001;Whittaker, Day, Curtis, & Fry, 2007;Whittaker et al, 2005). In this study, the cellular fatty acid (CFA) profiles of Y. pestis strains were compared to the CFA profiles of five closely related Yersinia species.…”

Tracing the food sources of isolated strains of Listeria monocytogenes through fatty acid profiles analysis

“…(Daneshvar, Douglas, & Weyant, 2001;Welch, 1991;Whittaker, 2009;Whittaker et al, 2005Whittaker et al, , 2007. In this study, the fatty acid profiles of the 22 L. monocytogenes strains were analyzed by MIS, results are showed in Table 3.…”