Cellular fatty acid compositions of an unidentified organism and a bacterium associated with cat scratch disease

Abstract

The cellular fatty acid composition of a gram-negative bacterium associated with cat scratch disease was determined by capillary gas chromatography-mass spectrometry (GC-MS). The fatty acid profile of this organism was distinct from those of other bacteria we have tested and was characterized by an unknown acid which was identified as 11-methyloctadec-12-enoic acid. The position of the branched methyl group in this acid was established by GC-MS of the reduced acid, and the location of the double bond was confirmed by GC-MS analysis of dimethyl disulfide derivatives. Another clinical isolate with no known relationship to cat scratch disease but with similar morphological and biochemical features had a similar fatty acid profile, including 11-methyloctadec-12-enoic acid.

Fatty Acids from a Glass Sponge Aulosaccus sp. Occurrence of New Cyclopropane‐Containing and Methyl‐Branched Acids

“…The new 5,13-dimethyl-tetradec-4-enoic acid seems to be a structural combination of 5-methyl-14:1Δ4 (a possible precursor of 7-methyl-16:1Δ6) [14] and 13-methyl-14:1Δ4 [18], previously found in demosponges. 9-Methyl-16:1Δ10 and 11-methyl-18:1Δ12, reported here, have been found in bacteria [19][20][21][22] and references cited therein]. FA with an allylic methyl group as well as cyclopropane FA (3.2 mol% in the FA mixture from Aulosaccus sp.…”

“…Six cis-cyclopropane acids, formal derivatives of monoenoic FA of the (n-7) family, were found in Aulosaccus sp., including common 9,10-methylene-16:0, 11,12-methylene-18:0, rare 13,14-methylene-20:0 (odd-numbered C 17 , C 19 , C 21 homologues) and three new acids 1a-c (evennumbered C 18 , C 20 , C 28 homologues). The 1 H-NMR spectra of the RP-HPLC fractions containing these acids (except for the minor C 18 (Fig.…”

Proposal of Afipia gen. nov., with Afipia felis sp. nov. (formerly the cat scratch disease bacillus), Afipia clevelandensis sp. nov. (formerly the Cleveland Clinic Foundation strain), Afipia broomeae sp. nov., and three unnamed genospecies

“…Cells were grown for 3 to 5 days. Cellular fatty acids were determined as described previously (23).…”

Cat Scratch Disease: the Rare Role of Afipia felis

“…Cellular fatty acid analysis showed the following composition: C 18:17C (50%), C 16:17C (6%), C 16:0 (4%), C 17:0cyc (6%), C 16:0 2OH (4%), C 18:19C (2%), C 18:0 (11%), C 19:0cyc 8C (14%), and C 12:0 3OH (1%) and also a component with an equivalent chain length of 18.08, an unsaturated branched-chain fatty acid methyl ester (11-methyloctadec-12-enoic acid). The latter is a unique fatty acid found in the type strain of A. felis and characterized by gas-liquid chromatography and chromatography-mass spectrometry (21).…”