1995
DOI: 10.1002/jemt.1070310206
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Cellular functions during activation and damage by pathogens: Immunogold studies of the interaction of bacterial endotoxins with target cells

Abstract: Bacterial endotoxins (lipopolysaccharides or LPS) are active components of Gram-negative bacteria that act on numerous cellular functions through the processes of cell activation and damage. The molecular mechanisms involved in the "endotoxic phenomenon" are not defined yet, although extensive studies have been carried out. Immunogold and electron microscopy (EM) have contributed to identify the primary target cells of endotoxins and the subcellular systems that receive the direct action of these bacterial age… Show more

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Cited by 24 publications
(19 citation statements)
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“…At the electron microscopic level, LPS micelles are identified on the basis of lamellar structure and use of immunocytochemistry (Kang et al, 1992;Risco and da Silva, 1995). The failure to depict the LPS with their typical lamellar arrangement in our micrographs could be a consequence of in vivo study or the limitation of our tissue-processing technique.…”
Section: Discussionmentioning
confidence: 97%
“…At the electron microscopic level, LPS micelles are identified on the basis of lamellar structure and use of immunocytochemistry (Kang et al, 1992;Risco and da Silva, 1995). The failure to depict the LPS with their typical lamellar arrangement in our micrographs could be a consequence of in vivo study or the limitation of our tissue-processing technique.…”
Section: Discussionmentioning
confidence: 97%
“…LPS is a bacterial endotoxin, which acts on numerous cellular functions through the processes of cell activation and damage. The molecular mechanisms involved in the "endotoxic phenomenon" are still not completely understood (Risco and Pinto da Silva, 1995).…”
Section: Introductionmentioning
confidence: 99%
“…In the literature, several agents have been used to promote macrophage activation, including the lipopolysaccharide (LPS) and the carrageenan (CAR) molecules (Risco and Pinto da Silva, 1995;Nacife et al, 2000;Tsuji et al, 2003). LPS is a bacterial endotoxin, which acts on numerous cellular functions through the processes of cell activation and damage.…”
Section: Introductionmentioning
confidence: 99%
“…The interaction of LPS with mammalian cells has been visualized by using immunogold labeling techniques followed by electron microscopic analysis (13)(14)(15)(16)(17)(18)(19). Although this method has helped identify the localization of LPS inside the intracellular organelles, some uncertainties in data interpretation may arise as the result of nonspecific interactions with Abs.…”
mentioning
confidence: 99%